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  4. N-Methylmaleimide

N-Methylmaleimide  (Synonyms: 1-Methyl-1H-pyrrole-2,5-dione)

Cat. No.: HY-W026277 Purity: 99.92%
COA
SDS
Handling Instructions Technical Support

N-Methylmaleimide (1-Methyl-1H-pyrrole-2,5-dione) is an electron-deficient alkene that can be used as a thiol blocking agent.

For research use only. We do not sell to patients.

N-Methylmaleimide

N-Methylmaleimide Chemical Structure

CAS No. : 930-88-1

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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N-Methylmaleimide Related Antibodies

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Description

N-Methylmaleimide (1-Methyl-1H-pyrrole-2,5-dione) is an electron-deficient alkene that can be used as a thiol blocking agent[1].

Molecular Weight

111.10

Formula

C5H5NO2
CAS No.
Appearance

Solid

Color

Off-white to yellow

SMILES

O=C(N1C)C=CC1=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Store at room temperature 3 years

In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (900.09 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 9.0009 mL 45.0045 mL 90.0090 mL
5 mM 1.8002 mL 9.0009 mL 18.0018 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.97%

SDS (615 KB)

COA (248 KB) HNMR (113 KB) RP-HPLC (190 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 9.0009 mL 45.0045 mL 90.0090 mL 225.0225 mL
5 mM 1.8002 mL 9.0009 mL 18.0018 mL 45.0045 mL
10 mM 0.9001 mL 4.5005 mL 9.0009 mL 22.5023 mL
15 mM 0.6001 mL 3.0003 mL 6.0006 mL 15.0015 mL
20 mM 0.4500 mL 2.2502 mL 4.5005 mL 11.2511 mL
25 mM 0.3600 mL 1.8002 mL 3.6004 mL 9.0009 mL
30 mM 0.3000 mL 1.5002 mL 3.0003 mL 7.5008 mL
40 mM 0.2250 mL 1.1251 mL 2.2502 mL 5.6256 mL
50 mM 0.1800 mL 0.9001 mL 1.8002 mL 4.5005 mL
60 mM 0.1500 mL 0.7501 mL 1.5002 mL 3.7504 mL
80 mM 0.1125 mL 0.5626 mL 1.1251 mL 2.8128 mL
100 mM 0.0900 mL 0.4500 mL 0.9001 mL 2.2502 mL
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N-Methylmaleimide Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

N-Methylmaleimide930-88-11-Methyl-1H-pyrrole-2,5-dioneBiochemical Assay ReagentsInhibitorinhibitorinhibit

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