Nampt activator-5
Nampt activator-5 is a NAMPT activator with a KD value of 6.19 μM. Nampt activator-5 activates the rate-limiting enzyme in NAD+ biosynthesis and promotes NAD+ production. Nampt activator-5 delays the senescence process of senescent hepatocytes, extends the lifespan of *Caenorhabditis elegans*, and alleviates age-related dysfunction and abnormal biomarkers in naturally aged mice. Nampt activator-5 can be used in aging research.
For research use only. We do not sell to patients.
- CAS No.: 3041005-21-1
- Formula: C20H18F2N4O3S
- Molecular Weight:432.44
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HUVEC | IC50 |
282.7 μM
Compound: C8
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Cytotoxicity against HUVEC cells assessed as reduction in cell viability incubated for 72 hrs by CCK-8 assay
Cytotoxicity against HUVEC cells assessed as reduction in cell viability incubated for 72 hrs by CCK-8 assay
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[PMID: 38367219] |
Nampt activator-5 (Compound C8) (0-12 μM) increases intracellular NAD+ levels in A549 cells to 184% at 10 μM, with levels rising in a concentration-dependent manner[1].
Nampt activator-5 increases intracellular NAD+ levels in HEK-293T cells in a concentration-dependent manner[1].
Nampt activator-5 (72 h) exhibits minimal cytotoxicity in HUVEC cells with an IC50 of 282.7 μM after 72 h, and antagonizes FK866-induced cytotoxicity[1].
Nampt activator-5 (20 μM; 24 h) effectively improves lysosomal senescence in Doxorubicin (HY-15142A)-induced senescent HL-7702 cells, normalizing the red/green fluorescence ratio to match young HL-7702 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Nampt activator-5 (20 mg/kg; i.p.; every 2 days; 2 months) improves age-related physical dysfunctions, reduces senescence biomarkers, and increases brain NAD+ levels in naturally aged female C57BL/6J mice[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:wild-type N2[1]
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Dosage:100 μM
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Administration:incorporation into NGM growth medium; continuous exposure
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Result:Extended the mean lifespan of C.
elegans by 8.37% compared to the control group, with a statistically significant difference.
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Animal Model:C57BL/6J (female, 20-month-old, naturally aged)[1]
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Dosage:20 mg/kg
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Administration:i.p.; every 2 days; 2 months
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Result:Improved muscle strength (grip strength/body weight ratio), hanging endurance (suspension duration × body weight), rotarod performance (running time, distance, maximum speed) compared to aged control mice, with results approaching those of young control mice.
Significantly reduced serum levels of CXCL1, IL-1β, MMP-1, p21, and p53 compared to aged control mice.
Reduced protein levels of p21, p53, and γ-H2AX in kidney tissue compared to aged control mice.
Significantly elevated NAD+ levels in the brain compared to aged control mice.
Chemical Information
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CAS No. 3041005-21-1
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Molecular Weight 432.44
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Formula C20H18F2N4O3S
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SMILES
O=C(NCC1=CC=NC=C1)NC2=CC=C(S(NCC3=C(C=CC(F)=C3)F)(=O)=O)C=C2
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Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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