Nampt activator-5
Nampt activator-5 is a NAMPT activator with a KD value of 6.19 μM. Nampt activator-5 activates the rate-limiting enzyme in NAD+ biosynthesis and promotes NAD+ production. Nampt activator-5 delays the senescence process of senescent hepatocytes, extends the lifespan of *Caenorhabditis elegans*, and alleviates age-related dysfunction and abnormal biomarkers in naturally aged mice. Nampt activator-5 can be used in aging research.
For research use only. We do not sell to patients.
- CAS No.: 3041005-21-1
- Formula: C20H18F2N4O3S
- Molecular Weight:432.44
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HUVEC | IC50 |
282.7 μM
Compound: C8
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Cytotoxicity against HUVEC cells assessed as reduction in cell viability incubated for 72 hrs by CCK-8 assay
Cytotoxicity against HUVEC cells assessed as reduction in cell viability incubated for 72 hrs by CCK-8 assay
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[PMID: 38367219] |
In Vitro
Nampt activator-5 (Compound C8) (0-12 μM) increases intracellular NAD+ levels in A549 cells to 184% at 10 μM, with levels rising in a concentration-dependent manner[1].
Nampt activator-5 increases intracellular NAD+ levels in HEK-293T cells in a concentration-dependent manner[1].
Nampt activator-5 (72 h) exhibits minimal cytotoxicity in HUVEC cells with an IC50 of 282.7 μM after 72 h, and antagonizes FK866-induced cytotoxicity[1].
Nampt activator-5 (20 μM; 24 h) effectively improves lysosomal senescence in Doxorubicin (HY-15142A)-induced senescent HL-7702 cells, normalizing the red/green fluorescence ratio to match young HL-7702 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
In Vivo
Nampt activator-5 (20 mg/kg; i.p.; every 2 days; 2 months) improves age-related physical dysfunctions, reduces senescence biomarkers, and increases brain NAD+ levels in naturally aged female C57BL/6J mice[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:wild-type N2[1]
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Dosage:100 μM
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Administration:incorporation into NGM growth medium; continuous exposure
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Result:Extended the mean lifespan of C.
elegans by 8.37% compared to the control group, with a statistically significant difference.
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Animal Model:C57BL/6J (female, 20-month-old, naturally aged)[1]
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Dosage:20 mg/kg
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Administration:i.p.; every 2 days; 2 months
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Result:Improved muscle strength (grip strength/body weight ratio), hanging endurance (suspension duration × body weight), rotarod performance (running time, distance, maximum speed) compared to aged control mice, with results approaching those of young control mice.
Significantly reduced serum levels of CXCL1, IL-1β, MMP-1, p21, and p53 compared to aged control mice.
Reduced protein levels of p21, p53, and γ-H2AX in kidney tissue compared to aged control mice.
Significantly elevated NAD+ levels in the brain compared to aged control mice.
Chemical Information
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CAS No. 3041005-21-1
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Molecular Weight 432.44
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Formula C20H18F2N4O3S
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SMILES
O=C(NCC1=CC=NC=C1)NC2=CC=C(S(NCC3=C(C=CC(F)=C3)F)(=O)=O)C=C2
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Senescence-associated β-galactosidase staining
Senescence-associated β-galactosidase staining detects β-galactosidase activity that is histochemically visible at pH 6. 0 in senescent cells, where X-gal cleavage produces an insoluble blue precipitate observable by bright-field microscopy. This activity reflects increased lysosomal β-galactosidase/lysosomal mass rather than a senescence-essential enzyme, because GLB1 depletion or genetic lysosomal β-galactosidase deficiency can abolish SA-β-gal staining while cells still undergo senescence. SA-β-gal was originally reported in senescent but not presenescent fibroblasts and keratinocytes, absent from quiescent fibroblasts and terminally differentiated keratinocytes, and increased with donor age in human skin samples. Because SA-β-gal can also appear in some non-senescent or tissue-specific contexts, interpretation should be paired with experimental controls and, when possible, independent senescence markers.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Nampt activator-5 Related Classifications
HY-162269 Related Classifications
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