2. Immunology/Inflammation
  3. Nuclear Factor of activated T Cells (NFAT)
  4. NFATc1-IN-1

NFATc1-IN-1 (compound A04) is a potent inhibitor of RANKL-induced osteoclast formation, with an IC50 of 1.57 μM. NFATc1-IN-1 shows anti-osteoclastogenic effects through reducing the RANKL-induced nuclear translocation of NFATc1. NFATc1-IN-1 can be used for osteoclastic diseases research.

For research use only. We do not sell to patients.

NFATc1-IN-1 Chemical Structure

NFATc1-IN-1 Chemical Structure

CAS No. : 1912422-56-0

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Based on 1 publication(s) in Google Scholar

NFATc1-IN-1 Related Antibodies

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1 Publications Citing Use of MCE NFATc1-IN-1

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Description

NFATc1-IN-1 (compound A04) is a potent inhibitor of RANKL-induced osteoclast formation, with an IC50 of 1.57 μM. NFATc1-IN-1 shows anti-osteoclastogenic effects through reducing the RANKL-induced nuclear translocation of NFATc1. NFATc1-IN-1 can be used for osteoclastic diseases research[1].

In Vitro

NFATc1-IN-1 (compound A04) (0-2.5 μM, 4 days) exhibits potent inhibitory activities on osteoclast formation and function, which eventually translates into decreased bone resorption[1].
NFATc1-IN-1 (1.5-2.5 μM, 24 h) blocks NFATc1 nuclear translocation and decreases the level of NFATc1[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

NFATc1-IN-1 Related Antibodies

Cell Viability Assay[1]

Cell Line: Osteoclast precursor RAW 264.7 cells
Concentration: 0, 0.5, 1.0, 1.5, 2.0 and 2.5 μM
Incubation Time: 4 days
Result: Markedly reduced the number and size of red TRAP-positive multinucleated osteoclasts at concentrations of 1.5, 2.0, and 2.5 μM. Significantly inhibited formation of osteoclasts in a dose-dependent manner (at 1.5, 2.0 and 2.5 μM), while showing no cytotoxic effects towards osteoclast precursor cells at concentrations of as high as 2.5 μM.

Immunofluorescence[1]

Cell Line: RAW264.7 cells
Concentration: 1.5 or 2.5 μM
Incubation Time: 24 h
Result: Blocked NFATc1 nuclear translocation and decreased the level of NFATc1.
Molecular Weight

375.11

Formula

C13H8F2INO2
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

OC1=C(C(NC2=C(F)C=C(I)C=C2)=O)C=C(F)C=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (333.24 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.6659 mL 13.3294 mL 26.6588 mL
5 mM 0.5332 mL 2.6659 mL 5.3318 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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This equation is commonly abbreviated as: C1V1 = C2V2

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Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.6659 mL 13.3294 mL 26.6588 mL 66.6471 mL
5 mM 0.5332 mL 2.6659 mL 5.3318 mL 13.3294 mL
10 mM 0.2666 mL 1.3329 mL 2.6659 mL 6.6647 mL
15 mM 0.1777 mL 0.8886 mL 1.7773 mL 4.4431 mL
20 mM 0.1333 mL 0.6665 mL 1.3329 mL 3.3324 mL
25 mM 0.1066 mL 0.5332 mL 1.0664 mL 2.6659 mL
30 mM 0.0889 mL 0.4443 mL 0.8886 mL 2.2216 mL
40 mM 0.0666 mL 0.3332 mL 0.6665 mL 1.6662 mL
50 mM 0.0533 mL 0.2666 mL 0.5332 mL 1.3329 mL
60 mM 0.0444 mL 0.2222 mL 0.4443 mL 1.1108 mL
80 mM 0.0333 mL 0.1666 mL 0.3332 mL 0.8331 mL
100 mM 0.0267 mL 0.1333 mL 0.2666 mL 0.6665 mL
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NFATc1-IN-1 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

NFATc1-IN-11912422-56-0Nuclear Factor of activated T Cells (NFAT)RAW 264.7 cellsanti-osteoclastogeneticosteoclastogenesissalicylanilide derivativeOsteoclastNFATc1anti-resorptiveRANKLInhibitorinhibitorinhibit

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