Nitrobenzylthioinosine (Synonyms: NBMPR)

Name: Nitrobenzylthioinosine
Brand: MedChemExpress
SKU: HY-W010936
Rating: 5.0 (3 reviews)

Cat. No.: HY-W010936 Purity: 98.57%: Data Sheet
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Nitrobenzylthioinosine is an ENT1 transporter inhibitor that binds to ENT1 transporter with high affinity. Nitrobenzylthioinosine is a photoaffinity probe for adenosine uptake sites in brain. Nitrobenzylthioinosine can cross the blood-brain barrier.

For research use only. We do not sell to patients.

CAS No. : 38048-32-7

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 3 publication(s) in Google Scholar

3 Publications Citing Use of MCE Nitrobenzylthioinosine

Cell Proliferation/Viability Assay

: Nitrobenzylthioinosine purchased from MedChemExpress. Usage Cited in: bioRxiv. 2026 Jan 27.; The inhibitor of hENT, Nitrobenzylthioinosine (NBMPR) (31.6–10,000 nM), enhanced the efficacy of DHODH inhibition in the presence of supraphysiological uridine levels in the ecMRT tumoroid model 103T.

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Adenosine A₁ receptor (A₁R) Adenosine A_2A receptor (A_2AR) Adenosine A_2B receptor (A_2BR) Adenosine A₃ receptor (A₃R)

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

ENT1 transporter^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
HEK293	IC₅₀	75 μM Compound: Nitrobenzylmercaptopurine riboside	TP_TRANSPORTER: inhibition of 9-(2-phosphonomethoxyethyl)adenine(PMEA) efflux (PMEA: 1 uM) in MRP4-expressing HEK293 cells TP_TRANSPORTER: inhibition of 9-(2-phosphonomethoxyethyl)adenine(PMEA) efflux (PMEA: 1 uM) in MRP4-expressing HEK293 cells	[PMID: 12695538]
K562	IC₅₀	7.6 nM Compound: NBMPR	Inhibition of ENT1 in human K562 cells by flow cytometric assay Inhibition of ENT1 in human K562 cells by flow cytometric assay	[PMID: 17636949]
U2OS	IC₅₀	2.6 nM Compound: 65407	Inhibition of the equilibrative nucleoside transporter (ENT1, SLC29A1) as assessed by GPCR-mediated changes in cell morphology using the impedance-based transporter activity through receptor activation (TRACT) assay (PubChem AID: 1745861) Inhibition of the equilibrative nucleoside transporter (ENT1, SLC29A1) as assessed by GPCR-mediated changes in cell morphology using the impedance-based transporter activity through receptor activation (TRACT) assay (PubChem AID: 1745861)	[PMID: 31551431]

In Vitro

Nitrobenzylthioinosine (0-25 μM) can inhibit uridine uptake by HeLa cells^[4].
Nitrobenzylthioinosine (10 μM, 72 h) combines with N-Phosphonacetyl-L-aspartic acid (PALA) can inhibit the viability of B16 melanoma cells^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Nitrobenzylthioinosine (50, 100 mg/kg, intraperitoneally injected, 4 times a day) combines with N-Phosphonacetyl-L-aspartic acid (PALA) can inhibit the growth of B16 melanoma^[5].
Nitrobenzylthioinosine (50 nM, intraventricular injection) can protect rat CA1 pyramidal neurons from ischemic death^[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

419.41

Formula

C₁₇H₁₇N₅O₆S

CAS No.

38048-32-7

Appearance

Solid

Color

White to off-white

SMILES

OC[C@@H]1[C@H]([C@H]([C@H](N2C=NC3=C(N=CN=C32)SCC4=CC=C([N+]([O-])=O)C=C4)O1)O)O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (119.22 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.3843 mL	11.9215 mL	23.8430 mL
5 mM	0.4769 mL	2.3843 mL	4.7686 mL
10 mM	0.2384 mL	1.1922 mL	2.3843 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (4.96 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (4.96 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (4.96 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.57%

Select Batch:

Data Sheet (276 KB) SDS (393 KB)

COA (249 KB) HNMR (280 KB) RP-HPLC (257 KB) MS (205 KB)

Handling Instructions (2659 KB)

References

[1]. Lauri Alanko, et al. Nitrobenzylthioinosine (NBMPR) binding and nucleoside transporter ENT1 mRNA expression after prolonged wakefulness and recovery sleep in the cortex and basal forebrain of rat. J Sleep Res. 2003 Dec;12(4):299-304. [Content Brief]

[2]. C M Anderson, et al. Ability of nitrobenzylthioinosine to cross the blood-brain barrier in rats. Neurosci Lett. 1996 Nov 29;219(3):191-4. [Content Brief]

[3]. P J Marangos, et al. [3H]nitrobenzylthioinosine is a photoaffinity probe for adenosine uptake sites in brain. Eur J Pharmacol. 1982 Dec 3;85(3-4):359-60. [Content Brief]

[4]. Paterson AR, et al. Inhibition of uridine uptake in HeLa cells by nitrobenzylthioinosine and related compounds. Mol Pharmacol. 1977 Nov;13(6):1014-23. [Content Brief]

[5]. Erlichman C, et al. Antitumor activity of N-phosphonacetyl-L-aspartic acid in combination with nitrobenzylthioinosine. Biochem Pharmacol. 1984 Oct 15;33(20):3177-81. [Content Brief]

[6]. Parkinson FE, et al. Effects of nitrobenzylthioinosine on neuronal injury, adenosine levels, and adenosine receptor activity in rat forebrain ischemia. J Neurochem. 2000 Aug;75(2):795-802. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.3843 mL	11.9215 mL	23.8430 mL	59.6075 mL
	5 mM	0.4769 mL	2.3843 mL	4.7686 mL	11.9215 mL
	10 mM	0.2384 mL	1.1922 mL	2.3843 mL	5.9608 mL
	15 mM	0.1590 mL	0.7948 mL	1.5895 mL	3.9738 mL
	20 mM	0.1192 mL	0.5961 mL	1.1922 mL	2.9804 mL
	25 mM	0.0954 mL	0.4769 mL	0.9537 mL	2.3843 mL
	30 mM	0.0795 mL	0.3974 mL	0.7948 mL	1.9869 mL
	40 mM	0.0596 mL	0.2980 mL	0.5961 mL	1.4902 mL
	50 mM	0.0477 mL	0.2384 mL	0.4769 mL	1.1922 mL
	60 mM	0.0397 mL	0.1987 mL	0.3974 mL	0.9935 mL
	80 mM	0.0298 mL	0.1490 mL	0.2980 mL	0.7451 mL
	100 mM	0.0238 mL	0.1192 mL	0.2384 mL	0.5961 mL

Nitrobenzylthioinosine Related Classifications

Neurological Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Nitrobenzylthioinosine38048-32-7NBMPRAdenosine ReceptorP1 receptorENT1transporteradenosineBBBneuroprotectivephotoaffinityprobeInhibitorinhibitorinhibit

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Nitrobenzylthioinosine (Synonyms: NBMPR)

Customer Review

Nitrobenzylthioinosine Related Antibodies

3 Publications Citing Use of MCE Nitrobenzylthioinosine

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•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Adenosine Receptor Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Nitrobenzylthioinosine Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Nitrobenzylthioinosine Related Classifications

Keywords:

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