Art. -Nr.: HY-103160A

EHNA hydrochloride

5 Cited Publications

Kundenbewertung

Based on 5 publication(s) in Google Scholar

EHNA hydrochloride is a potent and selective dual inhibitor of cyclic nucleotide phosphodiesterase 2 (PDE2)(IC₅₀=4 μM) and adenosine deaminase (ADA). EHNA hydrochloride exerts a concentration inhibition of the cGMP-stimulated PDE II (cGs-PDE)(IC₅₀:0.8 μM (human), 2 μM (porcine myocardium)), but has smaller inhibitory effect on the unstimulated PDE2 activity. EHNA hydrochloride play roles in mediating diverse pharmacological responses, including antiviral, antitumour and antiarrhythmic effects.

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Reinheit: 99.67%
CAS. Nr.: 58337-38-5
Formel: C₁₄H₂₄ClN₅O
Molecular Weight:313.83

Speicherung:

-20°C, sealed storage, away from moisture

* In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture)

EHNA hydrochloride Chemical Structure

CAS. Nr.: 58337-38-5

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Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- In Vitro
- Clinical Trial
Chemical Information
Publications (5)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target^[1]

PDE2	hPDE2A 0.8 μM (IC₅₀)

Alle Phosphodiesterase (PDE) Isoform-spezifische Produkte anzeigen :

In Vitro

EHNA completely ablates the ability of cyclic GMP to activate PDE2 activity, whilst having a much smaller inhibitory effect on the unstimulated PDE2 activity^[2].
EHNA exhibits normal Michaelian kinetics of inhibition for the cyclic GMP-stimulated PDE2 activity with Hill plots near unity^[2].
EHNA prevents dAdo degradation and increases mitochondrial dATP levels in fibroblasts^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS. Nr. 58337-38-5
Appearance Solid
Molecular Weight 313.83
Formel C₁₄H₂₄ClN₅O
Color White to light yellow
SMILES

CCCCCC[C@@](N1C2=C(C(N)=NC=N2)N=C1)([H])[C@H](O)C.Cl
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

-20°C, sealed storage, away from moisture

* In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture)

Publications (5)

All Bio/Physico-chemical AssayPK/PD AnalysisCell Proliferation/Viability Assay

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Journal Impact Factor

Nucleic Acids Res

Metabolic isotopic labeling of (deoxy)ribose enables dual neutral loss scanning for profiling nucleic acid modifications. [Abstract]

2026 Feb 24;54(5):gkag155. PMID: 41732913
Front Immunol

CD38+CD39+ NK cells associate with HIV disease progression and negatively regulate T cell proliferation. [Abstract]

2022 Oct 4:13:946871. PMID: 36268017

EHNA hydrochloride purchased from MedChemExpress. Usage Cited in: Front Immunol. 2022 Oct 4:13:946871. [Abstract]

EHNA hydrochloride (100 μM; 30 min)-mediated inhibition of adenosine deaminase (ADA) impairs the proliferation of CD4⁺ T cells, whereas CD8⁺ T cell proliferation remains unaffected.
Oncoimmunology

Overcoming the resistance of hepatocellular carcinoma to PD-1/PD-L1 inhibitor and the resultant immunosuppression by CD38 siRNA-loaded extracellular vesicles. [Abstract]

2022 Dec 26;12(1):2152635. PMID: 36605619

EHNA hydrochloride purchased from MedChemExpress. Usage Cited in: Oncoimmunology. 2022 Dec 26;12(1):2152635. [Abstract]

Adenosine production in HCCLM3 cells. The results showed that Culture medium of siNC- and siCD38-treated HCCLM3 cells without EHNA hydrochloride (100 μM) intervention showed gradually degraded adenosine and no accumulation, with no significant difference, while upon EHNA hydrochloride, adenosine accumulated in the medium and its content was decreased in response to siCD38 at different time points (6 h, 12 h, 18 h, and 24 h).
Eur J Pharmacol

Bay 60-7550, a PDE2 inhibitor, exerts positive inotropic effect of rat heart by increasing PKA-mediated phosphorylation of phospholamban. [Abstract]

2021 Jun 15:901:174077. PMID: 33798601

EHNA hydrochloride purchased from MedChemExpress. Usage Cited in: Eur J Pharmacol. 2021 Jun 15:901:174077. [Abstract]

The effects of EHNA hydrochloride (0.01, 0.1, 1, 10 μmol/L) were also presented in non-pacing and pacing modes in ex vivo isolated rat left hearts. EHNA: a classical PDE2 inhibitor. During the experiments, these different concentrations of EHNA from low to high concentration order were added to the perfusion solution and infused via retrograde perfusion of the coronary artery. The hemodynamic parameters were recorded when isolated heart contraction reached constant level with EHNA perfusions longer than 10 min for each concentration. Their intraventricular pressures were measured.
Biomed Chromatogr

Distribution and Pharmacokinetic Characteristics of Cordycepin in Rat: Investigated by UHPLC-HRMS/MS and Blood-Brain Synchronous Microdialysis. [Abstract]

2025 Apr;39(4):e70038. PMID: 40071940

EHNA hydrochloride purchased from MedChemExpress. Usage Cited in: Biomed Chromatogr. 2025 Apr;39(4):e70038. [Abstract]

Time-course curves of cordycepin in rat blood and brain after administration of cordycepin (10 mg/kg; i.p.) with EHNA hydrochloride (10 mg/kg; i.p.; single dose).

EHNA hydrochloride purchased from MedChemExpress. Usage Cited in: Biomed Chromatogr. 2025 Apr;39(4):e70038. [Abstract]

Time-course curves of 3′-deoxyinosine in rat blood and brain after administration of cordycepin (10 mg/kg; i.p.) with or without EHNA hydrochloride (10 mg/kg; i.p.; single dose).

Lösungsmittel & Löslichkeit

In Vitro:

H₂O : 100 mg/mL (318.64 mM; Need ultrasonic)

DMSO : 83.33 mg/mL (265.53 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.1864 mL

15.9322 mL

31.8644 mL

5 mM

0.6373 mL

3.1864 mL

6.3729 mL

10 mM

0.3186 mL

1.5932 mL

3.1864 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (6.63 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (6.63 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (6.63 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Working solution concentration: 0.22 mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact

MedChemExpress (MCE) .

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (278 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
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- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Podzuweit T, et al. Isozyme selective inhibition of cGMP-stimulated cyclic nucleotide phosphodiesterases by erythro-9-(2-hydroxy-3-nonyl) adenine.Cell Signal. 1995 Sep;7(7):733-8. [Content Brief]

[2]. Michie AM, et al. Rapid regulation of PDE-2 and PDE-4 cyclic AMP phosphodiesterase activity following ligation of the T cell antigen receptor on thymocytes: analysis using the selective inhibitors erythro-9-(2-hydroxy-3-nonyl)-adenine (EHNA) and rolipram.Cell Signal. 1996 Feb;8(2):97-110. [Content Brief]

[3]. Blázquez-Bermejo C, et al. Increased dNTP pools rescue mtDNA depletion in human POLG-deficient fibroblasts. FASEB J. 2019 Jun;33(6):7168-7179. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO / H₂O	1 mM	3.1864 mL	15.9322 mL	31.8644 mL	79.6610 mL
	5 mM	0.6373 mL	3.1864 mL	6.3729 mL	15.9322 mL
	10 mM	0.3186 mL	1.5932 mL	3.1864 mL	7.9661 mL
	15 mM	0.2124 mL	1.0621 mL	2.1243 mL	5.3107 mL
	20 mM	0.1593 mL	0.7966 mL	1.5932 mL	3.9830 mL
	25 mM	0.1275 mL	0.6373 mL	1.2746 mL	3.1864 mL
	30 mM	0.1062 mL	0.5311 mL	1.0621 mL	2.6554 mL
	40 mM	0.0797 mL	0.3983 mL	0.7966 mL	1.9915 mL
	50 mM	0.0637 mL	0.3186 mL	0.6373 mL	1.5932 mL
	60 mM	0.0531 mL	0.2655 mL	0.5311 mL	1.3277 mL
	80 mM	0.0398 mL	0.1992 mL	0.3983 mL	0.9958 mL
	100 mM	0.0319 mL	0.1593 mL	0.3186 mL	0.7966 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Keywords

EHNA hydrochloride

5 Cited Publications

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