CM-H2DCFDA
Based on 7 publication(s) in Google Scholar
CM-H2DCFDA is a derivative of H2DCFDA (HY-D0940). CM-H2DCFDA can be used to determine cellular oxidant levels (Ex/Em: 495/530 nm). CM-H2DCFDA is light-sensitive.
For research use only. We do not sell to patients.
- Purity: 91%
- CAS No.: 850013-49-9
- Formula: C27H19Cl3O8
- Molecular Weight:577.79
-
Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
Publications Citing Use of MedChemExpress (MCE) CM-H2DCFDA
More- J Hazard Mater. 2025 Dec 5:501:140718. [Abstract]
- J Transl Med. 2024 Apr 26;22(1):390. [Abstract]
- Cell Rep. 2025 May 7;44(5):115661. [Abstract]
- J Cell Commun Signal. 2025 Feb 19;19(1):e12059. [Abstract]
- Mol Med Rep. 2025 May;31(5):135. [Abstract]
- Front Public Health. 2023 Jul 13:11:1222762. [Abstract]
- PLoS One. 2024 Jul 11;19(7):e0306926. [Abstract]
Customer Validation & Images
-
Others
-
Cell Imaging/Staining
-
Cell Imaging/Staining
-
Cell Imaging/Staining
Biological Activity
Guide (The following is our recommended solution. This solution is merely a guideline and should be modified according to your specific needs.)
1. Prepare the storage solution and working solution
1.1 Prepare a 5 mM stock solution of CM-H2DCFDA using anhydrous DMSO.
1.2 Dilute the stored solution with pre-warmed serum-free cell culture medium or PBS to prepare a working solution of 1-10 μM CM-H2DCFDA.
Note: Please adjust the concentration of the CM-H2DCFDA working solution according to the actual situation, and prepare it as needed.
2. Cell Staining (Suspension Cells)
2.1 Centrifuge the cells and add PBS for two washes, each for 5 minutes. The cell density is 1×106 cells/mL.
2.2 Add 1 mL of the dye working solution and incubate at room temperature for 5 to 30 minutes.
2.3 400 grams, centrifuge for 3-4 minutes, then discard the supernatant.
2.4 Add PBS to wash the cells twice, for 5 minutes each time.
2.5 After resuspending the cells in 1 mL of serum-free medium or PBS, observe them using a fluorescence microscope or a flow cytometer.
3. Cell Staining (Monolayer Cells)
3.1 Cultivate adherent cells on sterile coverslips.
3.2 Remove the cover glass from the culture medium and aspirate away the excess medium.
3.3 Add 100 μL of the dye working solution and gently shake to ensure complete coverage of the cells. Incubate for 5 to 30 minutes.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 850013-49-9
-
Appearance Solid
-
Molecular Weight 577.79
-
Formula C27H19Cl3O8
-
Color White to off-white
-
SMILES
CC(OC1=C(Cl)C=C2C(C3=C(C=CC(CCl)=C3)C(OC(C)=O)=O)C4=CC(Cl)=C(OC(C)=O)C=C4OC2=C1)=O.CC(OC5=C(Cl)C=C6C(C7=C(C=C(CCl)C=C7)C(OC(C)=O)=O)C8=CC(Cl)=C(OC(C)=O)C=C8OC6=C5)=O
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (7)
-
Journal Impact Factor
-
Most Recent
-
J Hazard Mater
Species-specific impacts of fibrous microplastics on behavior, survival and oxidative stress in freshwater zooplankton. [Abstract]2025 Dec 5:501:140718. PMID: 41371136
CM-H2DCFDA purchased from MedChemExpress. Usage Cited in: J Hazard Mater. 2025 Dec 5:501:140718. [Abstract]
CM-H2DCFDA (9.62 µM). ROS levels in T. platyurus and B. calyciflorus after 24-h of exposure to fibrous MPs.
-
J Transl Med
CAV3 alleviates diabetic cardiomyopathy via inhibiting NDUFA10-mediated mitochondrial dysfunction. [Abstract]2024 Apr 26;22(1):390. PMID: 38671439
CM-H2DCFDA purchased from MedChemExpress. Usage Cited in: J Transl Med. 2024 Apr 26;22(1):390. [Abstract]
Representative images of DHE staining and ROS (CM-H2DCFDA) level quantification analysis of the hearts of db/db mice transfected or untransfected with AAV-CAV3 or AAV-shNDUFA10.
-
Cell Rep
The m5C methyltransferase NSUN2 promotes progression of acute myeloid leukemia by regulating serine metabolism. [Abstract]2025 May 7;44(5):115661. PMID: 40343793 -
J Cell Commun Signal
Moscatilin alleviates oxidative stress and inflammatory response of Müller cells in diabetic retinopathy through suppressing the p38 mitogen-activated protein kinase/c-Jun N-terminal kinase and nuclear factor kappa-B signaling pathways. [Abstract]2025 Feb 19;19(1):e12059. PMID: 39975983
CM-H2DCFDA purchased from MedChemExpress. Usage Cited in: J Cell Commun Signal. 2025 Feb 19;19(1):e12059. [Abstract]
Measurement of intracellular reactive oxygen species production by using the oxidation‐sensitive fluoroprobe CM‐H2DCFDA (10 μM) as a substrate.
-
Mol Med Rep
Obacunone inhibits ferroptosis through regulation of Nrf2 homeostasis to treat diabetic nephropathy. [Abstract]2025 May;31(5):135. PMID: 40116089
CM-H2DCFDA purchased from MedChemExpress. Usage Cited in: Mol Med Rep. 2025 May;31(5):135. [Abstract]
Levels of ROS (10 µM CM-H2DCFDA) in HK-2 cells from each group were measured using immunofluorescence.
-
Front Public Health
2023 Jul 13:11:1222762. PMID: 37521985 -
PLoS One
Therapeutic potential of salidroside in type I diabetic erectile dysfunction: Attenuation of oxidative stress and apoptosis via the Nrf2/HO-1 pathway. [Abstract]2024 Jul 11;19(7):e0306926. PMID: 38990890
Solvent & Solubility
DMSO : 3 mg/mL (5.19 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
-
Data Sheet (274 KB)
-
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
-
Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.7307 mL | 8.6537 mL | 17.3073 mL | 43.2683 mL |
| 5 mM | 0.3461 mL | 1.7307 mL | 3.4615 mL | 8.6537 mL |
CM-H2DCFDA Related Classifications
HY-D1713 Related Classifications
Powered by Bioz