Tempone-H
Based on 1 publication(s) in Google Scholar
Tempone-H may be used as a spin trap in chemical and biological systems to quantify peroxynitrite and superoxide radical formation. Ferric and cupric ions are effective oxidants of Tempone-H.
For research use only. We do not sell to patients.
- Purity: 98.0%
- CAS No.: 3637-11-4
- Formula: C9H17NO2
- Molecular Weight:171.24
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Tempone-H
More
Biological Activity
Using TEMPONE-H the obtained sensitivity in the detection of peroxynitrite or superoxide radicals is about 10-fold higher than using the spin traps DMPO or TMIO[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 3637-11-4
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Appearance Solid
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Molecular Weight 171.24
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Formula C9H17NO2
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Color Off-white to yellow
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SMILES
O=C1CC(C)(C)N(O)C(C)(C)C1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (1)
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Journal Impact Factor
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Most Recent
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ACS Appl Mater Interfaces
Multiple RONS-Loaded Plasma-Activated Ice Microneedle Patches for Transdermal Treatment of Psoriasis. [Abstract]2024 Sep 4;16(35):46123-46132. PMID: 39180585
Solvent & Solubility
DMSO : 100 mg/mL (583.98 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (274 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Dikalov S, et al. Quantification of peroxynitrite, superoxide, and peroxyl radicals by a new spin trap hydroxylamine 1-hydroxy-2,2,6,6-tetramethyl-4-oxo-piperidine. Biochem Biophys Res Commun. 1997 Jan 3;230(1):54-7. [Content Brief]
[2]. Dikalov SI, et al. Amyloid beta peptides do not form peptide-derived free radicals spontaneously, but can enhance metal-catalyzed oxidation of hydroxylamines to nitroxides. J Biol Chem. 1999 Apr 2;274(14):9392-9. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 5.8398 mL | 29.1988 mL | 58.3976 mL | 145.9939 mL |
| 5 mM | 1.1680 mL | 5.8398 mL | 11.6795 mL | 29.1988 mL | |
| 10 mM | 0.5840 mL | 2.9199 mL | 5.8398 mL | 14.5994 mL | |
| 15 mM | 0.3893 mL | 1.9466 mL | 3.8932 mL | 9.7329 mL | |
| 20 mM | 0.2920 mL | 1.4599 mL | 2.9199 mL | 7.2997 mL | |
| 25 mM | 0.2336 mL | 1.1680 mL | 2.3359 mL | 5.8398 mL | |
| 30 mM | 0.1947 mL | 0.9733 mL | 1.9466 mL | 4.8665 mL | |
| 40 mM | 0.1460 mL | 0.7300 mL | 1.4599 mL | 3.6498 mL | |
| 50 mM | 0.1168 mL | 0.5840 mL | 1.1680 mL | 2.9199 mL | |
| 60 mM | 0.0973 mL | 0.4866 mL | 0.9733 mL | 2.4332 mL | |
| 80 mM | 0.0730 mL | 0.3650 mL | 0.7300 mL | 1.8249 mL | |
| 100 mM | 0.0584 mL | 0.2920 mL | 0.5840 mL | 1.4599 mL |
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