NODA-FGLP21
NODA-FGLP21 is a tumor-targeting agent and a fibrinogen-like protein 1 (FGL1) binder. NODA-FGLP21 specifically binds to FGL1, and this binding is competitively inhibited by unlabeled FGLP21. NODA-FGLP21 can be used in the research of liver cancer.
For research use only. We do not sell to patients.
- Formula: C71H115N23O20S
- Molecular Weight:1642.88
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
NODA-FGLP21 (68Ga) (100 μg NODA-FGLP21 precursor, 370 MBq 68Ga; 10 min at 60 °C, 0.5-2 h at 37 °C) can be efficiently radiolabeled with high purity and specific activity, and maintains stability in PBS and serum for up to 2 h at 37 °C[1].
NODA-FGLP21 (68Ga) (37 kBq, 1 μM unlabeled FGLP21 for blocking; 30-120 min at 37 °C) shows specific, high uptake in FGL1-positive Huh7 human hepatocarcinoma cells (1.10 % AD/105 cells at plateau) and minimal uptake in FGL1-negative U87 MG human glioma cells, with uptake in Huh7 cells significantly reduced by unlabeled FGLP21[1].
NODA-FGLP21 (68Ga) (37 kBq, 0.01 nM-100 μM unlabeled FGLP21; 2 h at 37 °C) binds to FGL1 on Huh7 human hepatocarcinoma cells with an IC50 of 101.0 nM, indicating specific, moderate-affinity binding[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
NODA-FGLP21 (68Ga) (5.0 MBq; i.v.; single dose) shows low uptake in FGL1-negative U87 MG xenografts, with tumor uptake of 0.82% ID/g at 30 min post-injection, confirming its specificity for FGL1-expressing tumors[1].
NODA-FGLP21 (68Ga) (18.5 MBq; i.v.; single dose) causes no significant acute histopathological damage to major organs in healthy Balb/c mice[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:NCG mice (6-8 weeks old, subcutaneous implantation of Huh7 human hepatocarcinoma cells)[1]
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Dosage:5.0 MBq (68Ga-NODA-FGLP21); 10 mg/kg (unlabeled FGLP21, blocking studies)
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Administration:i.v.; single dose
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Result:Reached tumor uptake of 3.48% ID/g at 30 min post-injection, with a tumor-to-blood ratio of 2.37 and tumor-to-muscle ratio of 17.85.
Achieved tumor uptake of 2.85% ID/g at 60 min post-injection, with a tumor-to-blood ratio of 8.08 and tumor-to-muscle ratio of 21.5.
Attained tumor uptake of 1.70% ID/g at 120 min post-injection, with a tumor-to-blood ratio of 16.5 and tumor-to-muscle ratio of 32.5.
Reduced tumor uptake to 0.94% ID/g at 30 min post-injection when co-administered with unlabeled FGLP21.
Detected no significant radioactivity (less than 2% ID/g) in normal organs except kidneys, which showed uptake of 20.00% ID/g at 30 min post-injection.
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Animal Model:NCG mice (6-8 weeks old, subcutaneous implantation of U87 MG human glioma cells)[1]
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Dosage:5.0 MBq (68Ga-NODA-FGLP21)
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Administration:i.v.; single dose
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Result:Reached tumor uptake of 0.82% ID/g at 30 min post-injection, with a tumor-to-blood ratio of 0.70 and tumor-to-muscle ratio of 4.20.
Detected kidney uptake of 24.40% ID/g at 30 min post-injection; all other normal organs showed uptake less than 2% ID/g.
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Animal Model:Balb/c mice (6-8 weeks old)[1]
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Dosage:18.5 MBq (68Ga-NODA-FGLP21)
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Administration:i.v.; single dose
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Result:Observed no macroscopic lesions in major organs.
Detected no significant histopathological damage in major organs (heart, liver, spleen, lung, kidney) compared to saline-injected controls.
Chemical Information
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Molecular Weight 1642.88
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Formula C71H115N23O20S
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Sequence
NODA-Ala-Ala-Val-His-Leu-Arg-Asp-Arg-Ala-Leu
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Sequence Shortening
NODA-AAVHLRDRAL
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
NODA-FGLP21 Related Classifications
HY-P11931 Related Classifications
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