Nrf2-IN-1
Based on 11 publication(s) in Google Scholar
Nrf2-IN-1 is an inhibitor of nuclear factor-erythroid 2-related factor 2 (Nrf2). Nrf2-IN-1 is developed for the research of acute myeloid leukemia (AML).
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研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 99.88%
- CAS 番号: 1610022-76-8
- 分子式: C21H22ClN3O2
- 分子量:383.87
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保管条件:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
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MedChemExpress（MCE）の使用を引用している文献 Nrf2-IN-1
More- Int J Nanomedicine. 2026 Apr 11:21:585928. [Abstract]
- Clin Transl Med. 2024 Apr;14(4):e1661. [Abstract]
- Acupunct Herb Med. 2024 Nov 12.
- Clin Exp Hypertens. 2026 Dec 31;48(1):2607404. [Abstract]
- Ecotoxicol Environ Saf. 2025 Nov 15:307:119451. [Abstract]
- Cancer Immunol Immunother. 2023 Jul;72(7):2233-2244. [Abstract]
- Inflammation. 2025 Feb 22. [Abstract]
- Clin Exp Pharmacol Physiol. 2026 Jan;53(1):e70093. [Abstract]
- Arch Oral Biol. 2025 Oct:178:106364. [Abstract]
- Cells Tissues Organs. July 08, 2021.
- bioRxiv. 2026 Mar 28:2026.03.27.711928. [Abstract]
Customer Validation & Images
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WB
生物活性
Nrf2[1]
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A549 | IC50 |
1.82 μM
Compound: 4f
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Cytotoxicity against human A549 cells assessed as growth inhibition after 24 to 48 hrs by sulforhodamine B assay
Cytotoxicity against human A549 cells assessed as growth inhibition after 24 to 48 hrs by sulforhodamine B assay
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[PMID: 24996138] |
| THP-1 | IC50 |
5.33 μM
Compound: 5
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Cytotoxicity against human THP-1 cells incubated for 48 hrs by CCK8 assay
Cytotoxicity against human THP-1 cells incubated for 48 hrs by CCK8 assay
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[PMID: 39241669] |
Nrf2-IN-1 (Compound 4f) (10 μM) inhibits Nrf2 activation[1].
Nrf2-IN-1(1-20 μM; 48 hours) inhibits growth of the three AML cell[1].
Nrf2-IN-1 (5-10 μM; 48 hours) induces apoptosis of three AML cells in vitro[1].
Nrf2-IN-1 induces apoptotic signaling involving Bcl-2 and Bax[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:THP-1 cells, HL-60 cells, U937 cells
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Concentration:1 μM, 5 μM, 10 μM, 20 μM
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Incubation Time:48 hours
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Result:Inhibited growth of the three AML cell types at 5 μM, 10 μM or 20 μM for 48 hours.
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Cell Line:THP-1 cells, HL-60 cells, U937 cells
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Concentration:5 μM, 10 μM
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Incubation Time:48 hours
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Result:Induced apoptosis in AML cells in vitro.
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Cell Line:THP-1 cells, HL-60 cells, U937 cells
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Concentration:5 μM, 10 μM
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Incubation Time:48 hours
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Result:Triggered caspase-dependent apoptotic signaling in AML cells.
化学情報
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CAS 番号 1610022-76-8
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性状 Solid
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分子量 383.87
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分子式 C21H22ClN3O2
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Color White to off-white
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SMILES
O=C(NO)C1=CC(C2=CC=C(Cl)C=C2)=NN1CC3=CC=C(C(C)(C)C)C=C3
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (11)
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Journal Impact Factor
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Most Recent
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Int J Nanomedicine
Spatholobus suberectus Stem-Derived Extracellular Vesicle-Like Particles Attenuate Glucocorticoid-Induced Osteoporosis via NRF2/HO-1 Signaling. [Abstract]2026 Apr 11:21:585928. PMID: 41993783 -
Clin Transl Med
ACOD1, rather than itaconate, facilitates p62-mediated activation of Nrf2 in microglia post spinal cord contusion. [Abstract]2024 Apr;14(4):e1661. PMID: 38644791 -
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Clin Exp Hypertens
2026 Dec 31;48(1):2607404. PMID: 41447318 -
Ecotoxicol Environ Saf
MC-LR triggers trophoblast ferroptosis at the maternal-fetal interface by directly targeting PKM2 to reprogram metabolism. [Abstract]2025 Nov 15:307:119451. PMID: 41273830 -
Cancer Immunol Immunother
M2 tumor-associated macrophages resist to oxidative stress through heme oxygenase-1 in the colorectal cancer tumor microenvironment. [Abstract]2023 Jul;72(7):2233-2244. PMID: 36869896
Nrf2-IN-1 purchased from MedChemExpress. Usage Cited in: Cancer Immunol Immunother. 2023 Jul;72(7):2233-2244. [Abstract]
Nrf2-IN-1 (Nrf2 inhibitor; 5, 10, 20 μM; 48 h) decreases Nrf2 and HO-1 protein expressions in THP-1 cells in a dose dependent manner.
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Inflammation
4-Octyl Itaconate Attenuates Postmenopausal Osteoporosis by Inhibiting Ferroptosis and Enhancing Osteogenesis via the Nrf2 Pathway. [Abstract]2025 Feb 22. PMID: 39984770 -
Clin Exp Pharmacol Physiol
2026 Jan;53(1):e70093. PMID: 41267204 -
Arch Oral Biol
Taiwanese green propolis suppresses NLRP3 inflammasome activation and induces Nrf2/HO-1 pathway to reduce periodontal pathogen-induced endothelial inflammation. [Abstract]2025 Oct:178:106364. PMID: 40753822 -
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bioRxiv
2026 Mar 28:2026.03.27.711928. PMID: 41929138
溶剤 & 溶解度
DMSO : 250 mg/mL (651.26 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.17 mg/mL (5.65 mM); Clear solution
This protocol yields a clear solution of ≥ 2.17 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (21.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.17 mg/mL (5.65 mM); Clear solution
This protocol yields a clear solution of ≥ 2.17 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (21.7 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション
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取扱説明書 (2659 KB)
参考文献
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.6050 mL | 13.0252 mL | 26.0505 mL | 65.1262 mL |
| 5 mM | 0.5210 mL | 2.6050 mL | 5.2101 mL | 13.0252 mL | |
| 10 mM | 0.2605 mL | 1.3025 mL | 2.6050 mL | 6.5126 mL | |
| 15 mM | 0.1737 mL | 0.8683 mL | 1.7367 mL | 4.3417 mL | |
| 20 mM | 0.1303 mL | 0.6513 mL | 1.3025 mL | 3.2563 mL | |
| 25 mM | 0.1042 mL | 0.5210 mL | 1.0420 mL | 2.6050 mL | |
| 30 mM | 0.0868 mL | 0.4342 mL | 0.8683 mL | 2.1709 mL | |
| 40 mM | 0.0651 mL | 0.3256 mL | 0.6513 mL | 1.6282 mL | |
| 50 mM | 0.0521 mL | 0.2605 mL | 0.5210 mL | 1.3025 mL | |
| 60 mM | 0.0434 mL | 0.2171 mL | 0.4342 mL | 1.0854 mL | |
| 80 mM | 0.0326 mL | 0.1628 mL | 0.3256 mL | 0.8141 mL | |
| 100 mM | 0.0261 mL | 0.1303 mL | 0.2605 mL | 0.6513 mL |
Nrf2-IN-1 関連分類
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