2. Apoptosis
  3. MDM-2/p53
  4. NSC 66811

NSC 66811 

Cat. No.: HY-14967 Purity: 98.12%
COA Handling Instructions

NSC 66811 is a MDM2-p53 inhibitor, with a Ki of 120 nM for binding to MDM2.

For research use only. We do not sell to patients.

NSC 66811 Chemical Structure

NSC 66811 Chemical Structure

CAS No. : 6964-62-1

Size Price Stock Quantity
Solution
10 mM * 1 mL in DMSO USD 88 In-stock
Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
 USD 88 In-stock
Estimated Time of Arrival: December 31
Solid
5 mg USD 80 In-stock
Estimated Time of Arrival: December 31
10 mg   Get quote  
50 mg   Get quote  

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Customer Review

Based on 1 publication(s) in Google Scholar

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Description

NSC 66811 is a MDM2-p53 inhibitor, with a Ki of 120 nM for binding to MDM2[1].

In Vitro

NSC 66811 (0, 5, 10, 20 μM) dose-dependently induces the accumulation of p53, MDM2, and p21cip1/waf due to the functional activation of p53[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[1]

Cell Line: HCT-116 (p53+/+ and p53-/-) cells[1].
Concentration: 0, 5, 10, 20 μM.
Incubation Time: 48 h.
Result: Dose-dependently induced the accumulation of p53, MDM2, and p21cip1/waf proteins in the HCT-116 human colon cancer cell line with wild-type p53.
Had no effect on the levels for p53, MDM2, and p21cip1/waf protein in the isogenic HCT-116 p53-/- cell line.
Molecular Weight

340.42

Appearance

Solid

Formula

C23H20N2O
CAS No.
SMILES

OC1=C2N=C(C)C=CC2=CC=C1C(C3=CC=CC=C3)NC4=CC=CC=C4
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (293.75 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.9375 mL 14.6877 mL 29.3755 mL
5 mM 0.5875 mL 2.9375 mL 5.8751 mL
10 mM 0.2938 mL 1.4688 mL 2.9375 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: 2.5 mg/mL (7.34 mM); Suspended solution; Need ultrasonic

  • 2.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (7.34 mM); Clear solution

*All of the co-solvents are available by MCE.
Purity & Documentation

Purity: 98.12%

COA (249 KB) HNMR (332 KB) LCMS (125 KB)

Handling Instructions (2659 KB)
References
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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This equation is commonly abbreviated as: C1V1 = C2V2

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Keywords:

NSC 668116964-62-1NSC66811NSC-66811MDM-2/p53HCT-116MDM2p53Inhibitorinhibitorinhibit

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