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Cat. No.: HY-103706

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ROC-325

Name: ROC-325
Brand: MedChemExpress
SKU: HY-103706
Price: 59.00 USD
Availability: InStock
Rating: 5 (4 reviews)

4 Cited Publications

Customer Review

Based on 4 publication(s) in Google Scholar

ROC-325 is a potent and orally active autophagy inhibitor with a strong anticancer activity. ROC-325 induces the deacidification of lysosomes, accumulation of autophagosomes, and disrupted autophagic flux. ROC-325 also induces renal cell carcinoma apoptosis.

For research use only. We do not sell to patients.

Purity : 98.73%
CAS No.: 1859141-26-6
Formula: C₂₈H₂₇ClN₄OS
Molecular Weight:503.06

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

ROC-325 Chemical Structure

CAS No.: 1859141-26-6

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (4)
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

Autophagy^[1]

In Vitro

ROC-325 antagonizes renal cell carcinoma (RCC) growth and survival in an ATG5/7-dependent manner, induces apoptosis, and exhibits favorable selectivity. ROC-325 inhibits cells growth with IC₅₀ values of 4.9 μM, 11 μM, 4.6 μM, 5.4 μM, 7.4 μM, 11 μM, 8.2 μM, 5.8 μM, 5.0 μM, 11 μM, 8.4 μM and 6.0 μM for A498, A549, CFPAC-1, COLO-205, DLD-1, IGROV-1, MCF-7, MiaPaCa-2, NCI-H69, PC-3, RL and UACC-62 cells, respectively. ROC-325 induces hallmark features of autophagy inhibition and antagonizes autophagic flux^[1].
ROC-325 triggers a highly significant increase in cathepsin D (CTSD) levels. Treatment with 5 μM ROC-325 for 24 hours leads to the formation of LC3B punctae and a robust increase in LC3B levels in both A498 and 786-0 RCC cells. Immunoblotting analysis conducted in both A498 and 786-0 cells demonstrates that ROC-325 promotes a dose-dependent increase in LC3B expression in a manner that correlated with a corresponding increase in the levels of p62 and cathepsin D^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Oral administration of ROC-325 (25 mg/kg, 40 mg/kg, 50 mg/kg,) to mice bearing 786-0 RCC xenografts is well tolerated, significantly more effective at inhibiting tumor progression than Hydroxychloroquine, and inhibits autophagy in vivo^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Related Protocols :

Chemical Information

CAS No. 1859141-26-6
Appearance Solid
Molecular Weight 503.06
Formula C₂₈H₂₇ClN₄OS
Color Light yellow to orange
SMILES

O=C1C2=C(SC3=C1C=CC=C3)C(C)=CC=C2NCCN(CCNC4=CC=NC5=CC(Cl)=CC=C45)C
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (4)

All WBIFHistological Imaging/StainingIn Vivo Efficacy Study

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Journal Impact Factor

J Extracell Vesicles

Neuronal Extracellular Vesicles Carrying APOE Downregulate Filament Actin Polymerization Signaling to Inhibit Synapse Formation in Alzheimer's Disease. [Abstract]

2026 Mar;15(3):e70248. PMID: 41806350
Mater Today Nano

2026 Jul 21;35:100895.
Eur J Pharmacol

Autophagic inhibitor ROC-325 ameliorates glomerulosclerosis and podocyte injury via inhibiting autophagic flux in experimental FSGS mice. [Abstract]

2024 Nov 15:983:177007. PMID: 39307335

ROC-325 purchased from MedChemExpress. Usage Cited in: Eur J Pharmacol. 2024 Nov 15:983:177007. [Abstract]

Western blotting showed the expression of the p62, LC3A/B and GAPDH in MPCs treated with YAP siRNA or added ROC-325 with the concentration of 2.5 μM and 5 μM, respectively.

ROC-325 purchased from MedChemExpress. Usage Cited in: Eur J Pharmacol. 2024 Nov 15:983:177007. [Abstract]

Immunofluorescence staining for mRFP (red puncta) and GFP (green puncta) in the MPCs treated with ROC-325 (5 μM).

ROC-325 purchased from MedChemExpress. Usage Cited in: Eur J Pharmacol. 2024 Nov 15:983:177007. [Abstract]

Representative light microscopy of kidney sections by periodic acid–Schiff (PAS) staining from the mice treated with ROC-325 (50 mg/kg, i.g.).

ROC-325 purchased from MedChemExpress. Usage Cited in: Eur J Pharmacol. 2024 Nov 15:983:177007. [Abstract]

Representative images of the three experimental groups of mice and kidney. Body weight, kidney weight and kidney/body weight treated with ROC-325 (50 mg/kg, i.g.).
Res Sq

2026 Jan 9.

Solvent & Solubility

In Vitro:

DMSO : 4.55 mg/mL (9.04 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.9878 mL

9.9392 mL

19.8783 mL

5 mM

0.3976 mL

1.9878 mL

3.9757 mL

10 mM

---

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Dilution Calculator

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Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (279 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Carew JS, et al. Disruption of Autophagic Degradation with ROC-325 Antagonizes Renal Cell Carcinoma Pathogenesis. Clin Cancer Res. 2017 Jun 1;23(11):2869-2879. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.9878 mL	9.9392 mL	19.8783 mL	49.6959 mL
DMSO	5 mM	0.3976 mL	1.9878 mL	3.9757 mL	9.9392 mL

Bioz

Keywords

MOQ

Minimum order quantity

100 mg

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Amount: USD 0.00

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (4)
Solvent & Solubility
- In Vitro
Purity & Documentation
References

1/16
1/16

ROC-325 purchased from MedChemExpress. Usage Cited in: Eur J Pharmacol. 2024 Nov 15:983:177007. [Abstract]

Customer Validation
Western blotting showed the expression of the p62, LC3A/B and GAPDH in MPCs treated with YAP siRNA or added ROC-325 with the concentration of 2.5 μM and 5 μM, respectively.
1/16

ROC-325 purchased from MedChemExpress. Usage Cited in: Eur J Pharmacol. 2024 Nov 15:983:177007. [Abstract]

Customer Validation
Immunofluorescence staining for mRFP (red puncta) and GFP (green puncta) in the MPCs treated with ROC-325 (5 μM).
1/16

ROC-325 purchased from MedChemExpress. Usage Cited in: Eur J Pharmacol. 2024 Nov 15:983:177007. [Abstract]

Customer Validation
Representative light microscopy of kidney sections by periodic acid–Schiff (PAS) staining from the mice treated with ROC-325 (50 mg/kg, i.g.).
1/16

ROC-325 purchased from MedChemExpress. Usage Cited in: Eur J Pharmacol. 2024 Nov 15:983:177007. [Abstract]

Customer Validation
Representative images of the three experimental groups of mice and kidney. Body weight, kidney weight and kidney/body weight treated with ROC-325 (50 mg/kg, i.g.).

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ROC-325

Publications Citing Use of MedChemExpress (MCE) ROC-325