Nα-Benzoyl-L-arginine 4-nitroanilide hydrochloride
Based on 1 Customer Validation
Nα-Benzoyl-L-arginine 4-nitroanilide hydrochloride is a chromogenic peptide substrate used for trypsin and tryptase-like serine proteases. Nα-Benzoyl-L-arginine 4-nitroanilide hydrochloride releases p-nitroaniline upon hydrolysis, which can be detected by absorbance. Nα-Benzoyl-L-arginine 4-nitroanilide hydrochloride can be used in studies of A1PI degradation.
For research use only. We do not sell to patients.
- Purity : 99.92%
- CAS No.: 21653-40-7
- Formula: C19H23ClN6O4
- Molecular Weight:434.88
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
Nα-Benzoyl-L-arginine 4-nitroanilide hydrochloride serves as a chromogenic substrate for detecting the inactivation of A1PI by SAP-containing C. albicans supernatant[1].
Nα-Benzoyl-L-arginine 4-nitroanilide hydrochloride can be used to detect and monitor the serine protease activity of Cdtsp 2 during its purification from Crotalus durissus terrificus snake venom[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. .
Chemical Information
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CAS No. 21653-40-7
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Appearance Solid
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Molecular Weight 434.88
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Formula C19H23ClN6O4
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Color White to light yellow
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (229.95 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 5 mg/mL (11.50 mM; ultrasonic and warming and heat to 60°C)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.75 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (274 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 2.2995 mL | 11.4974 mL | 22.9948 mL | 57.4871 mL |
| 5 mM | 0.4599 mL | 2.2995 mL | 4.5990 mL | 11.4974 mL | |
| 10 mM | 0.2299 mL | 1.1497 mL | 2.2995 mL | 5.7487 mL | |
| DMSO | 15 mM | 0.1533 mL | 0.7665 mL | 1.5330 mL | 3.8325 mL |
| 20 mM | 0.1150 mL | 0.5749 mL | 1.1497 mL | 2.8744 mL | |
| 25 mM | 0.0920 mL | 0.4599 mL | 0.9198 mL | 2.2995 mL | |
| 30 mM | 0.0766 mL | 0.3832 mL | 0.7665 mL | 1.9162 mL | |
| 40 mM | 0.0575 mL | 0.2874 mL | 0.5749 mL | 1.4372 mL | |
| 50 mM | 0.0460 mL | 0.2299 mL | 0.4599 mL | 1.1497 mL | |
| 60 mM | 0.0383 mL | 0.1916 mL | 0.3832 mL | 0.9581 mL | |
| 80 mM | 0.0287 mL | 0.1437 mL | 0.2874 mL | 0.7186 mL | |
| 100 mM | 0.0230 mL | 0.1150 mL | 0.2299 mL | 0.5749 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Nα-Benzoyl-L-arginine 4-nitroanilide hydrochloride Related Classifications
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