Oliveroline
Oliveroline is an aporphine alkaloid with anticancer activity and a G2 DNA damage checkpoint inhibitor. Oliveroline enables cells to escape G2 phase arrest after DNA damage. Oliveroline can be used in research related to various cancers including non-small cell lung cancer, lung cancer, and fibrosarcoma.
For research use only. We do not sell to patients.
- CAS No.: 62560-99-0
- Formula: C18H17NO3
- Molecular Weight:295.33
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| MCF7 | IC50 |
45 μM
Compound: 1
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Inhibition of cell proliferation in MCF7 mp53 cells in absence of 6.5 Gy [60Co]-induced DNA damage
Inhibition of cell proliferation in MCF7 mp53 cells in absence of 6.5 Gy [60Co]-induced DNA damage
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[PMID: 17315964] |
| MCF7 | IC50 |
20 μM
Compound: 1
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Inhibition of cell proliferation in MCF7 mp53 cells in presence of 6.5 Gy [60Co]-induced DNA damage
Inhibition of cell proliferation in MCF7 mp53 cells in presence of 6.5 Gy [60Co]-induced DNA damage
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[PMID: 17315964] |
In Vitro
Oliveroline (compound 1) (0.01-100 μM; 8 h post-irradiation) inhibits the G2 DNA damage checkpoint in MCF-7 mp53 cells in a concentration-dependent manner[1].
Oliveroline (24 h pre-irradiation) inhibits proliferation of MCF-7 mp53 cells with an IC50 of 45 μM in the absence of DNA damage, and shows ~2.3-fold increased potency (IC50 = 20 μM) in cells treated with 6.5 Gy ionizing radiation[1].
Oliveroline inhibits the growth of human nasopharyngeal carcinoma (NPC-TW01), non-small cell lung carcinoma (NCI-H226), T cell leukemia (Jurkat), renal carcinoma (A498), lung carcinoma (A549), and fibrosarcoma (HT1080) cell lines, with the highest potency against A498 cells (IC50 = 3.68 μM) and IC50 values ranging from 3.68-13.28 μM across all lines[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 62560-99-0
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Molecular Weight 295.33
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Formula C18H17NO3
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SMILES
O[C@@H]1[C@]2([H])C3=C(CCN2C)C=C(OCO4)C4=C3C5=CC=CC=C51
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Genotoxicity/Mutagenicity Study
The bacterial reverse mutation assay detects point mutations that restore amino-acid prototrophy in auxotrophic Salmonella typhimurium or Escherichia coli tester strains; after exposure to a test article, mutagenic activity is read out as an increased number of revertant colonies on minimal agar compared with the vehicle control. The assay uses tester strains with different mutation targets so that base-substitution and frameshift mutagens can be detected, and testing is performed with and without exogenous mammalian metabolic activation because some chemicals require biotransformation to become mutagenic.
Purity & Documentation
References
[1]. Brastianos HC, et al. Inhibition of the G2 DNA damage checkpoint by oliveroline isolated from Duguetia odorata. Journal of natural products. 2007 Feb;70(2):287-8. [Content Brief]
[2]. Chan YY, et al. The constituents of Michelia compressa var. formosana and their bioactivities. International journal of molecular sciences. 2014 Jun 17;15(6):10926-35. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Oliveroline Related Classifications
HY-N19737 Related Classifications
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