p-Tolylmaleimide
Based on 1 Customer Validation
p-Tolylmaleimide (compound 9) is a naphthalimide derivative that has cytotoxic effects on cancer cells. p-Tolylmaleimide can arrest the cell cycle of human acute myeloid leukemia cells K562 in the sub-G0/G1 phase and induce apoptosis.
For research use only. We do not sell to patients.
- Purity: 98.0%
- CAS No.: 1631-28-3
- Formula: C11H9NO2
- Molecular Weight:187.19
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Bioactive Compound Library Max
HY-L181
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Bioactive Compound Library
HY-L001
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Apoptosis Compound Library
HY-L003
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Anti-Cancer Compound Library
HY-L025
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Covalent Screening Library
HY-L036
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Anti-Blood Cancer Compound Library
HY-L079
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Cysteine Targeted Covalent Library
HY-L153
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Cysteine Targeted Covalent Fragment Library
HY-L154
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Cell Death Library
HY-L162
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Anti-Hematopathy Compound Library
HY-L171
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MCE Bioactive Compound Library
HY-L001V
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Covalent Screening Library Plus
HY-L036P
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Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library
HY-L199
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High-Throughput Bioactive Compound Library
HY-L205
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Covalent Fragment Library
HY-L909
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| MCF7 | GI50 |
>10 μg/mL
Compound: 36
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Cytotoxicity against human MCF7 cells assessed as cell growth after 2 days by sulforhodamine B assay
Cytotoxicity against human MCF7 cells assessed as cell growth after 2 days by sulforhodamine B assay
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[PMID: 21481593] |
| NCI-H460 | GI50 |
>10 μg/mL
Compound: 36
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Cytotoxicity against human H460 cells assessed as cell growth after 2 days by sulforhodamine B assay
Cytotoxicity against human H460 cells assessed as cell growth after 2 days by sulforhodamine B assay
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[PMID: 21481593] |
| SF-268 | GI50 |
>10 μg/mL
Compound: 36
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Cytotoxicity against human SF268 cells assessed as cell growth after 2 days by sulforhodamine B assay
Cytotoxicity against human SF268 cells assessed as cell growth after 2 days by sulforhodamine B assay
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[PMID: 21481593] |
p-Tolylmaleimide inhibits human acute myeloid leukemia cell K562 with IC50s of 24.99 μM (24 h), 6.61 (48 h), and 12.10 (72 h) respectively. p-Tolylmaleimide for inhibits human acute myeloid leukemia cell Jurkat with IC50s of 27.16 μM (24 h), 15.51 (48 h), and 3.94 (72 h) respectively[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1631-28-3
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Appearance Solid
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Molecular Weight 187.19
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Formula C11H9NO2
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Color Light yellow to yellow
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SMILES
O=C(C=C1)N(C2=CC=C(C)C=C2)C1=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : ≥ 100 mg/mL (534.22 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (13.36 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (276 KB)
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SDS (555 KB)
- English - EN (555 KB)
- Français - FR (555 KB)
- Deutsch - DE (555 KB)
- Norwegian - NO (555 KB)
- Español - ES (555 KB)
- Swedish - SV (555 KB)
- Italian - IT (555 KB)
- Korean - KR (555 KB)
- Portuguese - PT (555 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 5.3422 mL | 26.7108 mL | 53.4217 mL | 133.5541 mL |
| 5 mM | 1.0684 mL | 5.3422 mL | 10.6843 mL | 26.7108 mL | |
| 10 mM | 0.5342 mL | 2.6711 mL | 5.3422 mL | 13.3554 mL | |
| 15 mM | 0.3561 mL | 1.7807 mL | 3.5614 mL | 8.9036 mL | |
| 20 mM | 0.2671 mL | 1.3355 mL | 2.6711 mL | 6.6777 mL | |
| 25 mM | 0.2137 mL | 1.0684 mL | 2.1369 mL | 5.3422 mL | |
| 30 mM | 0.1781 mL | 0.8904 mL | 1.7807 mL | 4.4518 mL | |
| 40 mM | 0.1336 mL | 0.6678 mL | 1.3355 mL | 3.3389 mL | |
| 50 mM | 0.1068 mL | 0.5342 mL | 1.0684 mL | 2.6711 mL | |
| 60 mM | 0.0890 mL | 0.4452 mL | 0.8904 mL | 2.2259 mL | |
| 80 mM | 0.0668 mL | 0.3339 mL | 0.6678 mL | 1.6694 mL | |
| 100 mM | 0.0534 mL | 0.2671 mL | 0.5342 mL | 1.3355 mL |
p-Tolylmaleimide Related Classifications
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