PACAP-38 (31-38), human, mouse, rat TFA

Name: PACAP-38 (31-38), human, mouse, rat TFA
Brand: MedChemExpress
SKU: HY-P1845A
Rating: 4.5 (1 reviews)

Cat. No.: HY-P1845A Purity: 99.59%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

PACAP-38 (31-38), human, mouse, rat TFA is a PAC₁ receptor activator and increases the α-secretase activity. PACAP-38 (31-38), human, mouse, rat TFA elevates cytosolic Ca²⁺, increases proliferation and increases phosphorylation of extracellular regulates kinase (ERK) and the epidermal growth factor receptor (EGFR). PACAP-38 (31-38), human, mouse, rat TFA demonstrates potent, efficacious, and sustained stimulatory effects on sympathetic neuronal NPY and catecholamine production. PACAP-38 (31-38), human, mouse, rat TFA can be used for neurotrophic and neuroprotective research.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

PACAP-38 (31-38), human, mouse, rat TFA Chemical Structure

Size	Price	Stock	Quantity
500 μg	USD 100	In-stock	Estimated Time of Arrival: December 31
1 mg	USD 150	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 450	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 670	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of PACAP-38 (31-38), human, mouse, rat TFA:

PACAP-38 (31-38), human, mouse, rat In-stock

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EGFR/ErbB1/HER1 ErbB2/HER2 ErbB3/HER3 ErbB4/HER4

Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

PACAP-38 (31-38), human, mouse, rat TFA (100 nM; 2 min; NCI-H838 cells) induces EGFR, HER2 and ERK tyrosine phosphorylation^[1].
PACAP-38 (31-38), human, mouse, rat TFA (100 nM; 30 min; NCI-H838 cells) induces EGFR tyrosine phosphorylation with generates ROS and increases ROS levels by 51%^[1].
PACAP-38 (31-38), human, mouse, rat TFA (10 nM; 48 h) stimulates the growth of NCI-H838 cells^[1].
PACAP-38 (31-38), human, mouse, rat TFA (300 nM; 4 h) stimulates generation of APPsα in neural cells^[2].
PACAP-38 (31-38), human, mouse, rat TFA (0.01-10 nM; HEK 293 cells) stimulates neural cells express endogenous PAC1 receptors by cAMP accumulation and by an increase in cytosolic free calcium and induces elevation of the intracellular Ca²⁺ concentration in a dose-dependent manner with an EC₅₀ value of 0.81 nM^[2].
PACAP-38 (31-38), human, mouse, rat TFA (0.01 nM; 48 h) elicits potent and efficacious stimulation of NPY secretion from SCG neuronal cultures^[3].
PACAP-38 (31-38), human, mouse, rat TFA (100 nM; 14 d) produces sustained stimulated NPY and catecholamine secretionmpathetic neurons^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	NCI-H838 cells
Concentration:	10 nM
Incubation Time:	48 hours
Result:	Increased the number of NCI-H838 cells by 72%.

Western Blot Analysis^[1]

Cell Line:	NCI-H838 cells
Concentration:	100 nM
Incubation Time:	2 min
Result:	Increased tyrosine phosphorylation of the EGFR, HER2, and ERK by 377, 299 and 216%, respectively.

Molecular Weight

1176.29

Formula

C₄₉H₈₄F₃N₁₇O₁₃

Appearance

Solid

Color

White to off-white

Sequence

Tyr-Lys-Gln-Arg-Val-Lys-Asn-Lys-NH2

Sequence Shortening

YKQRVKNK-NH2

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (85.01 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	0.8501 mL	4.2507 mL	8.5013 mL
5 mM	0.1700 mL	0.8501 mL	1.7003 mL
10 mM	0.0850 mL	0.4251 mL	0.8501 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (85.01 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.59%

Select Batch:

Data Sheet (277 KB) SDS (252 KB)

COA (250 KB) RP-HPLC (252 KB)

Handling Instructions (2659 KB)

References

[1]. Moody TW, et, al. PAC1 regulates receptor tyrosine kinase transactivation in a reactive oxygen species-dependent manner. Peptides. 2019 Oct;120:170017. [Content Brief]

[2]. Kojro E, et, al. The neuropeptide PACAP promotes the alpha-secretase pathway for processing the Alzheimer amyloid precursor protein. FASEB J. 2006 Mar;20(3):512-4. [Content Brief]

[3]. Braas KM, et, al. Pituitary adenylate cyclase-activating polypeptides, PACAP-38 and PACAP-27, regulation of sympathetic neuron catecholamine, and neuropeptide Y expression through activation of type I PACAP/VIP receptor isoforms. Ann N Y Acad Sci. 1996 De [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	0.8501 mL	4.2507 mL	8.5013 mL	21.2533 mL
	5 mM	0.1700 mL	0.8501 mL	1.7003 mL	4.2507 mL
	10 mM	0.0850 mL	0.4251 mL	0.8501 mL	2.1253 mL
	15 mM	0.0567 mL	0.2834 mL	0.5668 mL	1.4169 mL
	20 mM	0.0425 mL	0.2125 mL	0.4251 mL	1.0627 mL
	25 mM	0.0340 mL	0.1700 mL	0.3401 mL	0.8501 mL
	30 mM	0.0283 mL	0.1417 mL	0.2834 mL	0.7084 mL
	40 mM	0.0213 mL	0.1063 mL	0.2125 mL	0.5313 mL
	50 mM	0.0170 mL	0.0850 mL	0.1700 mL	0.4251 mL
	60 mM	0.0142 mL	0.0708 mL	0.1417 mL	0.3542 mL
	80 mM	0.0106 mL	0.0531 mL	0.1063 mL	0.2657 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.