Polyinosinic-polycytidylic acid potassium
Based on 63 publication(s) in Google Scholar
Polyinosinic-polycytidylic acid potassium (Poly(I:C) potassium) is a synthetic analog of double-stranded RNA and an agonist of toll-like receptor 3 (TLR3) and retinoic acid inducible gene I (RIG-I)-like receptors (RIG-I and MDA5). Polyinosinic-polycytidylic acid sodium can be used as a vaccine adjuvant to enhance innate and adaptive immune responses, and to alter the tumor microenvironment. Polyinosinic-polycytidylic acid potassium can directly trigger cancer cells to undergoApoptosis.
For research use only. We do not sell to patients.
- CAS No.: 31852-29-6
- Formula: (C10H13N4O8P)x.(C9H14N3O8P)x.xK
-
Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
- Purity & Documentation
- References
-
Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Polyinosinic-polycytidylic acid potassium
More- Signal Transduct Target Ther. 2026 Feb 13;11(1):54. [Abstract]
- Signal Transduct Target Ther. 2025 Dec 23;10(1):410. [Abstract]
- Nature. 2025 Jul;643(8072):810-819. [Abstract]
- Imeta. 2026 Feb 24.
- Cell Metab. 2026 May 5;38(5):963-980.e12. [Abstract]
- Immunity. 2025 Sep 9;58(9):2320-2335.e9. [Abstract]
- Nat Cell Biol. 2025 Apr;27(4):683-695. [Abstract]
- J Extracell Vesicles. 2025 May;14(5):e70083. [Abstract]
- Autophagy. 2024 Oct;20(10):2133-2145. [Abstract]
- Nat Commun. 2025 Jun 3;16(1):5140. [Abstract]
- Nat Commun. 2025 Feb 24;16(1):1909. [Abstract]
- Hepatology. 2025 Nov 7. [Abstract]
- ACS Nano. 2025 Feb 4;19(4):4195-4212. [Abstract]
- Acta Pharm Sin B. 2025 Oct;15(10):5431-5443. [Abstract]
- J Exp Clin Cancer Res. 2025 Feb 7;44(1):45. [Abstract]
- Adv Sci (Weinh). 2026 Jun 2:e75921. [Abstract]
- Adv Sci (Weinh). 2026 Mar 18:e11012. [Abstract]
- Chem Eng J. 2021 Aug 15:418:129392. [Abstract]
- Biosens Bioelectron. 2026 Oct 15:310:118829. [Abstract]
- J Exp Med. 2026 Apr 6;223(4):e20250344. [Abstract]
- Cell Commun Signal. 2026 Feb 9;24(1):168. [Abstract]
- Mol Ther. 2025 Dec 3;33(12):6146-6159. [Abstract]
- Cell Death Discov. 2025 Jul 25;11(1):345. [Abstract]
- J Transl Med. 2025 Nov 10;23(1):1252. [Abstract]
- Cancer Immunol Res. 2024 Oct 1;12(10):1438-1451. [Abstract]
- Clin Transl Med. 2024 Sep;14(9):e70027. [Abstract]
- Cell Rep. 2026 Apr 3;45(4):117222. [Abstract]
- Cell Rep. 2026 Mar 30;45(4):117179. [Abstract]
- Food Front. 2025 Nov 6.
- Nutrients. 2025 Dec 19;18(1):21. [Abstract]
- Int Immunopharmacol. 2026 May 1:176:116468. [Abstract]
- Int Immunopharmacol. 2025 Feb 6:147:114033. [Abstract]
- mBio. 2026 Jun 10;17(6):e0042326. [Abstract]
- Cell Signal. 2025 Aug:132:111807. [Abstract]
- Virol Sin. 2023 Oct;38(5):741-754. [Abstract]
- Virol Sin. 2023 Jun;38(3):335-343. [Abstract]
- J Innate Immun. 2025 Jul 28:1-27. [Abstract]
- Poult Sci. 2026 May 20;105(8):107155. [Abstract]
- FASEB J. 2024 Mar 31;38(6):e23551. [Abstract]
- Fish Shellfish Immunol. 2026 Feb:169:111053. [Abstract]
- J Virol. 2025 Aug 19;99(8):e0049825. [Abstract]
- Curr Issues Mol Biol. 2025 May 14;47(5):361. [Abstract]
- J Funct Foods. 2026 May 30;142:107364.
- Vet Res. 2025 Nov 5;56(1):210. [Abstract]
- Vet Res. 2024 Sep 27;55(1):115. [Abstract]
- Cancer Med. 2023 Jul;12(14):15676-15690. [Abstract]
- Chem Biol Drug Des. 2025 Nov;106(5):e70196. [Abstract]
- Chem Biol Drug Des. 2023 Nov;102(5):1110-1120. [Abstract]
- Vet Microbiol. 2026 Feb:313:110834. [Abstract]
- Curr Microbiol. 2024 Aug 6;81(9):296. [Abstract]
- Virology. 2026 Jun:619:110878. [Abstract]
- Transbound Emerg Dis. 2026;2026(1):e5789277. [Abstract]
- World J Tradit Chin Med. 2025 Oct 28.
- Clin Cosmet Investig Dermatol. 2024 May 14:17:1093-1105. [Abstract]
- Biochem Biophys Res Commun. 2024 Apr 16:704:149661. [Abstract]
- Dev Comp Immunol. 2025 Dec:173:105527. [Abstract]
- Dev Comp Immunol. 2025 Sep 29:172:105483. [Abstract]
- Transpl Immunol. 2026 Feb:94:102346. [Abstract]
- Patent. US20250235423A1.
- bioRxiv. 2024 September 23.
- bioRxiv. 2024 Dec 8:2024.12.07.627371. [Abstract]
- Res Sq. 2024 Nov 14.
- SSRN. 2023 Apr 29.
Customer Validation & Images
-
IP
-
RT-PCR
-
RT-PCR
-
RT-PCR
-
IF
Biological Activity
|
TLR3 |
Polyinosinic-polycytidylic acid potassium (20 ng/mL; 24 hours; WM793, WM278, WM239A, WM9 and 1205Lu cells) inhibits cell growth[1].
Polyinosinic-polycytidylic acid potassium (200 ng/mL; 24 hours; 1205Lu cells) induces apoptosis in 1205Lu cells[1].
Polyinosinic-polycytidylic acid potassium (3 ng/mL; 24 hours; 1205Lu cells) induces IFN-β expression in melanoma cells. Silencing of RIG-I and MDA-5 confirmed that induction of IFN-β by Polyinosinic-polycytidylic acid required RIG-I and MDA-5, respectively, and that required IPS-1[1].
Polyinosinic-polycytidylic acid potassium is prepared for injection by resuspending in sterile saline, heating to 50 °C at a concentration of 2 mg/ml to ensure complete solubilisation and then allowing to cool naturally to room temperature to ensure proper annealing of double-stranded RNA. Poly I:C is stored at -20 °C until use[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:WM793, WM278, WM239A, WM9 and 1205Lu cells
-
Concentration:20 ng/mL
-
Incubation Time:24 hours
-
Result:Strongly reduced viability from 100% in controls to 20%–50% within 24 hours.
-
Cell Line:1205Lu cells
-
Concentration:200 ng/mL
-
Incubation Time:24 hours
-
Result:Induced apoptosis in 1205Lu cells.
-
Cell Line:WM793, WM278, WM239A, WM9 and 1205Lu cells
-
Concentration:3 ng/mL
-
Incubation Time:24 hours
-
Result:Induced IFN-β expression in melanoma cells.
-
Cell Line:1205Lu cells
-
Concentration:5 ng/mL
-
Incubation Time:24 hours
-
Result:Revealed active subunits of caspase-9 and caspase-8 in melanoma cells.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Animal Model:NOD/SCID immunodeficient mice (1205Lu cells)[1]
-
Dosage:50 µg
-
Administration:I.v.; on days 3, 6, and 9 after tumor inoculation
-
Result:Reduced the size of metastases and the total amount of tumor tissue, and the level of human DNA was 50% lower in mice.
Chemical Information
-
CAS No. 31852-29-6
-
Formula (C10H13N4O8P)x.(C9H14N3O8P)x.xK
-
SMILES
O[C@@H]1[C@H](O)[C@@H](COP(O)(O)=O)O[C@H]1N2C3=C(C(N=CN3)=O)N=C2.O[C@@H]4[C@H](O)[C@@H](COP(O)(O)=O)O[C@H]4N5C(N=C(C=C5)N)=O.[x].[x]
-
Synonyms
Poly(I:C) potassium
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications (63)
-
Journal Impact Factor
-
Most Recent
-
Signal Transduct Target Ther
2026 Feb 13;11(1):54. PMID: 41680122 -
Signal Transduct Target Ther
Oncolytic adenovirus delivery of neoantigens sensitizes low-mutation tumors to anti-PD-1 therapy and prevents metastasis. [Abstract]2025 Dec 23;10(1):410. PMID: 41429778 -
Nature
2025 Jul;643(8072):810-819. PMID: 40369065 -
-
Cell Metab
Psoriasis modulates inflammatory bowel disease risk and intestinal epithelium lipid metabolism via IL-1β-producing macrophages. [Abstract]2026 May 5;38(5):963-980.e12. PMID: 41856104 -
Immunity
Spatiotemporal dynamics of CXCL10 encode contextual immune information revealed by the genetically encoded fluorescent sensor. [Abstract]2025 Sep 9;58(9):2320-2335.e9. PMID: 40818452 -
Nat Cell Biol
Selective translational control by PABPC1 phase separation regulates blast crisis and therapy resistance in chronic myeloid leukaemia. [Abstract]2025 Apr;27(4):683-695. PMID: 40102686 -
J Extracell Vesicles
Cancer-Specific RNA Modifications in Tumour-Derived Extracellular Vesicles Promote Tumour Growth. [Abstract]2025 May;14(5):e70083. PMID: 40326665 -
Autophagy
2024 Oct;20(10):2133-2145. PMID: 38762760
Polyinosinic-polycytidylic acid potassium purchased from MedChemExpress. Usage Cited in: Autophagy. 2024 Oct;20(10):2133-2145. [Abstract]
HEK293T cells were transfected with HA-8A or HA-NSs at a dose of 2 μg for 24 h, and then transfected with poly(I:C) for 24 h. Co-IP with HA antibodies and evaluation of LC3, TBK1 and TRIM25 with western blot using IgG as control.
Polyinosinic-polycytidylic acid potassium purchased from MedChemExpress. Usage Cited in: Autophagy. 2024 Oct;20(10):2133-2145. [Abstract]
HEK293T cells were transfected with pCAGGS, HA-NSs and HA-8A, HA-PxxP, HA-DWP, or HA-WxxL at a dose of 2 μg for 24 h, and then transfected with poly(I:C) for 24 h. IFNB and IFIT1 mRNA levels were analyzed with RT-qPCR.
Polyinosinic-polycytidylic acid potassium purchased from MedChemExpress. Usage Cited in: Autophagy. 2024 Oct;20(10):2133-2145. [Abstract]
HeLa cells were transfected with pCAGGS, HA-8A or HA-NSs at a dose of 2 μg for 24 h, and then transfected with poly(I:C) for 24 h. IFNB, IFIT1 and CXCL10 mRNA levels were analyzed with RT-qPCR.
Polyinosinic-polycytidylic acid potassium purchased from MedChemExpress. Usage Cited in: Autophagy. 2024 Oct;20(10):2133-2145. [Abstract]
BECN1 knockout HeLa cells were transfected with pCAGGS, HA-8A or HA-NSs for 24 h, and then transfected with poly(I:C) for 24 h. IFNB, IFIT1 and CXCL10 mRNA levels were analyzed with RT-qPCR.
-
Nat Commun
De novo design of protein condensation inhibitors by targeting an allosteric site of cGAS. [Abstract]2025 Jun 3;16(1):5140. PMID: 40461475 -
Nat Commun
Identification of splenic IRF7 as a nanotherapy target for tele-conditioning myocardial reperfusion injury. [Abstract]2025 Feb 24;16(1):1909. PMID: 39994192 -
Hepatology
GPC3-based circular RNA vaccine suppresses hepatocellular carcinoma progression by activating adaptive immune responses. [Abstract]2025 Nov 7. PMID: 41201153 -
ACS Nano
2025 Feb 4;19(4):4195-4212. PMID: 39846120 -
Acta Pharm Sin B
Spatiotemporally delivery of Cas9 ribonucleoprotein/DNAzyme logic systems using near-infrared upconversion nanomachine for precise immunotherapy. [Abstract]2025 Oct;15(10):5431-5443. PMID: 41132842 -
J Exp Clin Cancer Res
VSIG4+ tumor-associated macrophages mediate neutrophil infiltration and impair antigen-specific immunity in aggressive cancers through epigenetic regulation of SPP1. [Abstract]2025 Feb 7;44(1):45. PMID: 39920772 -
Adv Sci (Weinh)
NMI Regulates Adipose Adaptive Thermogenesis Through TLR4/IRF3 Signaling to Promote Obesity. [Abstract]2026 Jun 2:e75921. PMID: 42228052 -
Adv Sci (Weinh)
RNF213 Is an Interferon-Stimulated Gene That Targets Influenza A Virus NP and Activates MDA5 to Restrict Infection. [Abstract]2026 Mar 18:e11012. PMID: 41849692 -
Chem Eng J
Inhalable nanovaccine with biomimetic coronavirus structure to trigger mucosal immunity of respiratory tract against COVID-19. [Abstract]2021 Aug 15:418:129392. PMID: 33762883
Polyinosinic-polycytidylic acid potassium purchased from MedChemExpress. Usage Cited in: Chem Eng J. 2021 Aug 15:418:129392. [Abstract]
Immunofluorescence staining photographs of lung sections. Scale bars, 100 μm. (I: PBS, II: Poly(I:C), III: PL-Poly(I:C), IV: NL-Poly(I:C), V: PL-Poly(I:C)/RBD, VI: NL-Poly(I:C)/RBD).
-
Biosens Bioelectron
In situ spatiotemporal tracking of endogenous protein dynamics with allosteric genetically encoded biosensor in living cells. [Abstract]2026 Oct 15:310:118829. PMID: 42184477 -
J Exp Med
Defects in intron recycling suppress the antiviral response via a mechanism of intronic endogenous dsRNA. [Abstract]2026 Apr 6;223(4):e20250344. PMID: 41817448 -
Cell Commun Signal
Grass carp reovirus VP35 hijacks DHX15 into phase-separated inclusion bodies to evade host antiviral immunity. [Abstract]2026 Feb 9;24(1):168. PMID: 41664180 -
Mol Ther
Targeting O-GlcNAcylated METTL3 impedes MDS/AML progression via diminishing SRSF1 m6A modification. [Abstract]2025 Dec 3;33(12):6146-6159. PMID: 40914805 -
Cell Death Discov
RIPK1 S213E mutant suppresses RIPK1-dependent cell death by preventing interactions with RIPK3 and CASP8. [Abstract]2025 Jul 25;11(1):345. PMID: 40715038 -
J Transl Med
Truncated APC impairs innate immune response by targeting MAVS on mitochondria in colorectal cancer. [Abstract]2025 Nov 10;23(1):1252. PMID: 41214683 -
Cancer Immunol Res
The E3 ubiquitin ligase FBXO38 maintains the antitumor function of natural killer cells by sustaining IL-15R signaling. [Abstract]2024 Oct 1;12(10):1438-1451. PMID: 38990095 -
Clin Transl Med
IBR1, a novel endogenous IFIH1-binding dsRNA, governs IFIH1 activation and M1 macrophage polarisation in ARDS. [Abstract]2024 Sep;14(9):e70027. PMID: 39313944 -
Cell Rep
2026 Apr 3;45(4):117222. PMID: 41934636 -
Cell Rep
4-octyl itaconate inhibits cytokine-mediated inflammation via alkylation of TYK2 and JAK1. [Abstract]2026 Mar 30;45(4):117179. PMID: 41915469 -
-
Nutrients
Aqueous Extract of Fructus Choerospondiatis Peel Suppresses Vascular Inflammation and Alleviates Atherosclerosis via AKT/c-FOS/IL-6 Axis. [Abstract]2025 Dec 19;18(1):21. PMID: 41515139 -
Int Immunopharmacol
Minimolide F alleviates inflammatory diseases by specifically targeting STING and blocking IRF3 recruitment. [Abstract]2026 May 1:176:116468. PMID: 41819671 -
Int Immunopharmacol
A VZV-gE subunit vaccine decorated with mPLA elicits protective cellular immmune responses against varicella-zoster virus. [Abstract]2025 Feb 6:147:114033. PMID: 39799738 -
mBio
Lipid droplets and small extracellular vesicles interplay in Japanese encephalitis virus non-lytic release. [Abstract]2026 Jun 10;17(6):e0042326. PMID: 42159429 -
Cell Signal
2025 Aug:132:111807. PMID: 40239727 -
Virol Sin
RIPK3 promotes hantaviral replication by restricting JAK-STAT signaling without triggering necroptosis. [Abstract]2023 Oct;38(5):741-754. PMID: 37633447 -
Virol Sin
Antibiotic-induced gut bacteria depletion has no effect on HBV replication in HBV immune tolerance mouse model. [Abstract]2023 Jun;38(3):335-343. PMID: 37141990 -
J Innate Immun
Impact of ATP Synthase Subunit β on TLR Signaling Pathway in Promoting Airway Remodeling and Heterogeneity of Small Airway Epithelial Cells in Chronic Obstructive Pulmonary Disease. [Abstract]2025 Jul 28:1-27. PMID: 40720940 -
Poult Sci
Duck plague virus US2 promotes p62-mediated autophagic degradation of RIG-I to suppress antiviral signaling. [Abstract]2026 May 20;105(8):107155. PMID: 42190475 -
FASEB J
2024 Mar 31;38(6):e23551. PMID: 38489235 -
Fish Shellfish Immunol
Cloning, functional characterization of PfLectin_C, a novel C-type lectin gene in pearl oyster Pinctada fucata, and its roles in immune defense and transplantation immunity. [Abstract]2026 Feb:169:111053. PMID: 41344455 -
J Virol
PEDV Nsp14 induces mitophagy-mediated degradation of MAVS to antagonize host innate immunity and facilitate viral proliferation. [Abstract]2025 Aug 19;99(8):e0049825. PMID: 40631894 -
Curr Issues Mol Biol
2025 May 14;47(5):361. PMID: 40699760 -
-
Vet Res
TRIM7 suppresses transmissible gastroenteritis virus replication by targeting the degradation of N protein and activating RIG-I-mediated type I IFN antiviral response. [Abstract]2025 Nov 5;56(1):210. PMID: 41194212 -
Vet Res
2024 Sep 27;55(1):115. PMID: 39334325 -
Cancer Med
Inhibition of FGFR3 upregulates MHC-I and PD-L1 via TLR3/NF-kB pathway in muscle-invasive bladder cancer. [Abstract]2023 Jul;12(14):15676-15690. PMID: 37283287 -
Chem Biol Drug Des
Investigation Into the Inhibition of Ferroptosis by Praeruptorin-A in Sepsis Based on Network Pharmacology. [Abstract]2025 Nov;106(5):e70196. PMID: 41195620 -
Chem Biol Drug Des
Praeruptorin A inhibits the activation of NF-κB pathway and the expressions of inflammatory factors in poly (I:C)-induced RAW264.7 cells. [Abstract]2023 Nov;102(5):1110-1120. PMID: 37500542 -
Vet Microbiol
miR-17195 promotes infectious bronchitis virus proliferation and macrophage-mediated inflammation via the PLCβ2-TAK1 axis. [Abstract]2026 Feb:313:110834. PMID: 41447967 -
Curr Microbiol
Poly I: C Alleviated Duck Intestinal Injury Infected with Duck Viral Enteritis by Inhibiting Apoptosis. [Abstract]2024 Aug 6;81(9):296. PMID: 39105989 -
Virology
2026 Jun:619:110878. PMID: 41880901 -
Transbound Emerg Dis
Porcine Rotavirus NSP4 Inhibits Type I Interferon Production via NRBF2-Mediated Autophagic Degradation of MDA-5. [Abstract]2026;2026(1):e5789277. PMID: 42338383 -
-
Clin Cosmet Investig Dermatol
Effect of Chloroquine on Type 2 Inflammatory Response in MC903-Induced Atopic Dermatitis Mice. [Abstract]2024 May 14:17:1093-1105. PMID: 38765196 -
Biochem Biophys Res Commun
Sequential administration of delta-tocotrienol ameliorates radiation-induced myelosuppression in mice and non-human primates through inducing G-CSF production. [Abstract]2024 Apr 16:704:149661. PMID: 38417343 -
Dev Comp Immunol
Genome-wide analysis unveils the differentiated "external" and "internal" lectin system in the tropical sea cucumber Holothuria leucospilota. [Abstract]2025 Dec:173:105527. PMID: 41274536 -
Dev Comp Immunol
Porcine IFI44L inhibits transmissible gastroenteritis virus (TGEV) replication by activating IFN-Ⅰ signaling pathway. [Abstract]2025 Sep 29:172:105483. PMID: 41033382 -
Transpl Immunol
TLR3 stimulation rejuvenates aged mesenchymal stem cells and restores immunosuppressive function in graft-versus-host disease. [Abstract]2026 Feb:94:102346. PMID: 41520686 -
-
-
bioRxiv
2024 Dec 8:2024.12.07.627371. PMID: 39677789 -
-
Purity & Documentation
References
[1]. Besch R, et al. Proapoptotic signaling induced by RIG-I and MDA-5 results in type I interferon-independent apoptosis in human melanoma cells. J Clin Invest. 2009 Aug;119(8):2399-411. [Content Brief]
[2]. Cheng YS, et al. Anticancer function of polyinosinic-polycytidylic acid. Cancer Biol Ther. 2010 Dec 15;10(12):1219-23. [Content Brief]
[3]. Robert Field, et al. Systemic challenge with the TLR3 agonist poly I:C induces amplified IFNalpha/beta and IL-1beta responses in the diseased brain and exacerbates chronic neurodegeneration. Brain Behav Immun. 2010 Aug;24(6):996-1007. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Polyinosinic-polycytidylic acid potassium Related Classifications
HY-134958 Related Classifications
Powered by Bioz