1. Immunology/Inflammation Apoptosis
  2. Toll-like Receptor (TLR) Apoptosis
  3. Polyinosinic-polycytidylic acid potassium

Polyinosinic-polycytidylic acid potassium  (Synonyms: Poly(I:C) potassium)

Cat. No.: HY-134958
Handling Instructions

Polyinosinic-polycytidylic acid potassium (Poly(I:C) potassium) is a synthetic analog of double-stranded RNA and an agonist of toll-like receptor 3 (TLR3) and retinoic acid inducible gene I (RIG-I)-like receptors (RIG-I and MDA5). Polyinosinic-polycytidylic acid sodium can be used as a vaccine adjuvant to enhance innate and adaptive immune responses, and to alter the tumor microenvironment. Polyinosinic-polycytidylic acid potassium can directly trigger cancer cells to undergoApoptosis.

For research use only. We do not sell to patients.

Polyinosinic-polycytidylic acid potassium Chemical Structure

Polyinosinic-polycytidylic acid potassium Chemical Structure

CAS No. : 31852-29-6

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Description

Polyinosinic-polycytidylic acid potassium (Poly(I:C) potassium) is a synthetic analog of double-stranded RNA and an agonist of toll-like receptor 3 (TLR3) and retinoic acid inducible gene I (RIG-I)-like receptors (RIG-I and MDA5). Polyinosinic-polycytidylic acid sodium can be used as a vaccine adjuvant to enhance innate and adaptive immune responses, and to alter the tumor microenvironment. Polyinosinic-polycytidylic acid potassium can directly trigger cancer cells to undergoApoptosis[1][2][3].

IC50 & Target[1]

TLR3

 

In Vitro

Polyinosinic-polycytidylic acid potassium (20 ng/mL; 24 hours; WM793, WM278, WM239A, WM9 and 1205Lu cells) inhibits cell growth[1].
Polyinosinic-polycytidylic acid potassium (200 ng/mL; 24 hours; 1205Lu cells) induces apoptosis in 1205Lu cells[1].
Polyinosinic-polycytidylic acid potassium (3 ng/mL; 24 hours; 1205Lu cells) induces IFN-β expression in melanoma cells. Silencing of RIG-I and MDA-5 confirmed that induction of IFN-β by Polyinosinic-polycytidylic acid required RIG-I and MDA-5, respectively, and that required IPS-1[1].
Polyinosinic-polycytidylic acid potassium is prepared for injection by resuspending in sterile saline, heating to 50 °C at a concentration of 2 mg/ml to ensure complete solubilisation and then allowing to cool naturally to room temperature to ensure proper annealing of double-stranded RNA. Poly I:C is stored at -20 °C until use[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: WM793, WM278, WM239A, WM9 and 1205Lu cells
Concentration: 20 ng/mL
Incubation Time: 24 hours
Result: Strongly reduced viability from 100% in controls to 20%–50% within 24 hours.

Apoptosis Analysis[1]

Cell Line: 1205Lu cells
Concentration: 200 ng/mL
Incubation Time: 24 hours
Result: Induced apoptosis in 1205Lu cells.

RT-PCR[1]

Cell Line: WM793, WM278, WM239A, WM9 and 1205Lu cells
Concentration: 3 ng/mL
Incubation Time: 24 hours
Result: Induced IFN-β expression in melanoma cells.

Western Blot Analysis[1]

Cell Line: 1205Lu cells
Concentration: 5 ng/mL
Incubation Time: 24 hours
Result: Revealed active subunits of caspase-9 and caspase-8 in melanoma cells.
In Vivo

Polyinosinic-polycytidylic acid potassium (50 µg; i.v.; on days 3, 6, and 9 after tumor inoculation) shows antitumor activity in NOD/SCID immunodeficient mice[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: NOD/SCID immunodeficient mice (1205Lu cells)[1]
Dosage: 50 µg
Administration: I.v.; on days 3, 6, and 9 after tumor inoculation
Result: Reduced the size of metastases and the total amount of tumor tissue, and the level of human DNA was 50% lower in mice.
Formula

(C10H13N4O8P)x.(C9H14N3O8P)x.xK

CAS No.
SMILES

O[C@@H]1[C@H](O)[C@@H](COP(O)(O)=O)O[C@H]1N2C3=C(C(N=CN3)=O)N=C2.O[C@@H]4[C@H](O)[C@@H](COP(O)(O)=O)O[C@H]4N5C(N=C(C=C5)N)=O.[x].[x]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation
References
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Polyinosinic-polycytidylic acid potassium Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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