2. Immunology/Inflammation
  3. Toll-like Receptor (TLR)
  4. CL075

CL075 (3M002) is a selective TLR8 agonist with immunomodulating properties. CL075 triggers a MyD88-dependent signaling pathway to elicit production of inflammatory cytokines and type I interferons (IFNs) via activation of NF-κB and IRF7, respectively.

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CL075 Chemical Structure

CL075 Chemical Structure

CAS No. : 256922-53-9

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Customer Review

Based on 3 publication(s) in Google Scholar

CL075 Related Antibodies

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Description

CL075 (3M002) is a selective TLR8 agonist with immunomodulating properties. CL075 triggers a MyD88-dependent signaling pathway to elicit production of inflammatory cytokines and type I interferons (IFNs) via activation of NF-κB and IRF7, respectively[1][2][3].

IC50 & Target

TLR8

 

Cellular Effect
Cell Line Type Value Description References
HEK293 EC50
1.32 μM
Compound: 40, CL075
Agonist activity at human TLR8 expressed in HEK293 cells assessed as induction of NF-kappaB activity by measuring extracellular secreted alkaline phosphatase by HEK-blue reporter gene assay
Agonist activity at human TLR8 expressed in HEK293 cells assessed as induction of NF-kappaB activity by measuring extracellular secreted alkaline phosphatase by HEK-blue reporter gene assay
[PMID: 22924757]
In Vitro

CL075 (2 μM; 4 h) induces the production of IL-6, IL-8, and IFN-γ in rabbit splenocytes[1].
? CL075 (2 μM; 48 h) induces the total IgM production and cell proliferation in rabbit splenocytes[1].
? CL075 (2 μM; 7 h) activates the rabTLR8 in 293 cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

CL075 Related Antibodies
In Vivo

CL075 (5 μM (0.5 mL); s.c.) activates a mild antigen-specific antibody response in rabbits[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

243.33

Formula

C13H13N3S
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

NC1=NC2=CC=CC=C2C(S3)=C1N=C3CCC
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 22 mg/mL (90.41 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.1096 mL 20.5482 mL 41.0965 mL
5 mM 0.8219 mL 4.1096 mL 8.2193 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.25%

SDS (393 KB)

COA (254 KB) HNMR (160 KB) RP-HPLC (194 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.1096 mL 20.5482 mL 41.0965 mL 102.7411 mL
5 mM 0.8219 mL 4.1096 mL 8.2193 mL 20.5482 mL
10 mM 0.4110 mL 2.0548 mL 4.1096 mL 10.2741 mL
15 mM 0.2740 mL 1.3699 mL 2.7398 mL 6.8494 mL
20 mM 0.2055 mL 1.0274 mL 2.0548 mL 5.1371 mL
25 mM 0.1644 mL 0.8219 mL 1.6439 mL 4.1096 mL
30 mM 0.1370 mL 0.6849 mL 1.3699 mL 3.4247 mL
40 mM 0.1027 mL 0.5137 mL 1.0274 mL 2.5685 mL
50 mM 0.0822 mL 0.4110 mL 0.8219 mL 2.0548 mL
60 mM 0.0685 mL 0.3425 mL 0.6849 mL 1.7124 mL
80 mM 0.0514 mL 0.2569 mL 0.5137 mL 1.2843 mL
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CL075 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

CL075256922-53-93M002CL 075CL-075Toll-like Receptor (TLR)TLR8immunomodulatinginflammatorycytokinesIFNsNF-κBIRF7Inhibitorinhibitorinhibit

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