2. Apoptosis Protein Tyrosine Kinase/RTK
  3. ROS Kinase Apoptosis
  4. PRDX1-IN-1

PRDX1-IN-1 is a selective inhibtor of PRDX1 with an IC50 value of 0.164 μM. PRDX1-IN-1 can be used in researches related to cancer.PRDX1-IN-1 promots intracellular ROS accumulation, and inhibits the proliferation, invasion and migration of cancer cells besides inducing apoptosis. PRDX1-IN-1 could be used in cancer research.

For research use only. We do not sell to patients.

PRDX1-IN-1 Chemical Structure

PRDX1-IN-1 Chemical Structure

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10 mg USD 1400 In-stock
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Based on 1 publication(s) in Google Scholar

PRDX1-IN-1 Related Antibodies

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Description

PRDX1-IN-1 is a selective inhibtor of PRDX1 with an IC50 value of 0.164 μM. PRDX1-IN-1 can be used in researches related to cancer.PRDX1-IN-1 promots intracellular ROS accumulation, and inhibits the proliferation, invasion and migration of cancer cells besides inducing apoptosis. PRDX1-IN-1 could be used in cancer research[1].

IC50 & Target

IC50:0.164μM(potent peroxiredoxin 1,PRDX1)[1]
In Vitro

PRDX1-IN-1 inhibites the proliferation activities of the human lung cancer cells A549, lung cancer cell lines (LTEP-a-2 and H1975), human breast cancer cell line (MDA-MB-231), human hepatoma cell line (SK-Hep-1) with the IC50 values of 1.92 μM, 2.93 μM, 1.99 μM, 2.67 μM, 2.42μM, respectively[1].
PRDX1-IN-1 (compound 7e)(2 μM or 4 μM, 24 h) promotes intracellular ROS accumulation, and inhibits the invasion and migration of human lung cancer cells A549[1].
PRDX1-IN-1 (2 μM or 4 μM, 24 h) induces the apoptosis of A549 cells[1].
PRDX1-IN-1 (2 μM or 4 μM, 6 h) suppresses the key signaling pathways (AKT and ERK) and promotes the expression of apoptosis-related proteins (cleaved caspase-3/8 and cleaved PARP) in A549 cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

PRDX1-IN-1 Related Antibodies

Proliferation assay[1]

Cell Line: lung cancer cell lines (LTEP-a-2 and H1975), human breast cancer cell line (MDA-MB-231), human hepatoma cell line (SK-Hep-1)
Concentration: 0.5–10 μM
Incubation Time: 48 h
Result: Inhibited the proliferation activities of cancer cells A549, LTEP-a-2, H1975, MDA-MB-231, SK-Hep-1.

Apoptosis assay [1]

Cell Line: human lung cancer cells A549
Concentration: 2 μM or 4 μM
Incubation Time: 24 h
Result: Increased the ratio of the total number of early (annexin-V+/PI− ) and late (annexin-V+/PI+) apoptotic cells significantly.

WB assay[1]

Cell Line: A549 cell
Concentration: 2 μM or 4 μM
Incubation Time: 6 h
Result: Decreased the phosphorylation levels of PI3K, AKT, C-RAF and ERK.

Matrigel invasion assay[1]

Cell Line: A549 cell
Concentration: 2 μM or 4 μM
Incubation Time: 24 - 48 h
Result: Inhibted the cell matrigel and invasion.
In Vivo

PRDX1-IN-1 (0.5 or 1 mg/kg, intraperitoneal(i.p.), 19 days, every day) inhibited tumor growth in a mouse model of lung cancer[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57BL/6J male mice injected with Lewis cell (lung cancer)[1]
Dosage: 0.5 or 1 mg/kg
Administration: intraperitoneal injection (i.p.), every day for 19 days.
Result: Inhibited tumor growth, with the tumor growth inhibition (TGI) values of 77.47% and 69.89% in the groups of 1 mg/kg and 0.5 mg/kg, respectively.
Induced the changes in morphological characteristics of tumor cells, including cell agglutination, contraction, and nuclear chromatin marginalization.
Molecular Weight

713.95

Formula

C46H55N3O4
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(OC1=C(C)C2=CC=C([C@](CC[C@]3(C)[C@@]4([H])C[C@@](C(NCC5=C(C)N=C(C)C(C)=N5)=O)(C)CC3)(C)[C@@]4(C)CC6)[C@]6(C)C2=CC1=O)/C=C/C7=CC=CC=C7
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (140.07 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.4007 mL 7.0033 mL 14.0066 mL
5 mM 0.2801 mL 1.4007 mL 2.8013 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

=
Concentration (final)

C2

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Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: 2.5 mg/mL (3.50 mM); Clear solution; Need ultrasonic

    This protocol yields a clear solution of 2.5 mg/mL.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: 2.5 mg/mL (3.50 mM); Clear solution; Need ultrasonic

    This protocol yields a clear solution of 2.5 mg/mL. If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.4007 mL 7.0033 mL 14.0066 mL 35.0165 mL
5 mM 0.2801 mL 1.4007 mL 2.8013 mL 7.0033 mL
10 mM 0.1401 mL 0.7003 mL 1.4007 mL 3.5016 mL
15 mM 0.0934 mL 0.4669 mL 0.9338 mL 2.3344 mL
20 mM 0.0700 mL 0.3502 mL 0.7003 mL 1.7508 mL
25 mM 0.0560 mL 0.2801 mL 0.5603 mL 1.4007 mL
30 mM 0.0467 mL 0.2334 mL 0.4669 mL 1.1672 mL
40 mM 0.0350 mL 0.1751 mL 0.3502 mL 0.8754 mL
50 mM 0.0280 mL 0.1401 mL 0.2801 mL 0.7003 mL
60 mM 0.0233 mL 0.1167 mL 0.2334 mL 0.5836 mL
80 mM 0.0175 mL 0.0875 mL 0.1751 mL 0.4377 mL
100 mM 0.0140 mL 0.0700 mL 0.1401 mL 0.3502 mL
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PRDX1-IN-1 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

PRDX1-IN-1ROS KinaseApoptosisPRDX1 InhibitorROScell matrigel and invasionlung cancerInhibitorinhibitorinhibit

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