Propamocarb hydrochloride
Based on 1 Customer Validation
Propamocarb hydrochloride is an orally active phenylalanine ammonia-lyase (PAL) inducer and oomycete membrane biosynthesis inhibitor that can be present in synthetic formulations. Propamocarb hydrochloride is used in research on blight, gut microbiota dysbiosis and metabolic disorders, cardiovascular diseases, and potato late blight.
For research use only. We do not sell to patients.
- Purity: 99.26%
- CAS No.: 25606-41-1
- Formula: C9H21ClN2O2
- Molecular Weight:224.73
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Storage:Pure form -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
Propamocarb hydrochloride significantly induces the expression of PAL, POD, CYP, AMP, ANP, and UDP genes in cucumber fruit peel, with the highest induction for POD reaching a 70.83-fold increase at 6 h post-treatment[1].
Propamocarb hydrochloride significantly increases the activities of PAL (to 101.8 nmol·h-1·mg-1 protein) and POD (to 106.1 U·g-1 FW) in cucumber fruit peel[1].
Propamocarb hydrochloride significantly increases acetylbromide-extractable Lignin (HY-111830) content in cucumber fruit peel, reaching 80.76 mg/g dry weight, which is a 41.1% increase compared to the control[1].
Propamocarb (1.8-10 mg/mL; 5-7 days) hydrochloride provided minimal inhibition of mycelial growth for the 71 P. nicotianae isolates tested, with the most sensitive isolate showing 34.8% relative growth at 10 mg/mL[2].
Propamocarb (1-100 mg/mL; 5-7 days) hydrochloride is a poor inhibitor of P. nicotianae mycelial growth, with EC50 values ranging from 2.2 to 90.1 mg/mL across the nine isolates and a mean of 20.6 mg/mL[2].
Propamocarb (5-50,000 µg/mL; 10 h) hydrochloride potently suppressed sporangium production in P. nicotianae isolates, with EC50 values ranging from 133.8 to 481.3 µg/mL and complete inhibition at 50,000 µg/mL[2].
Propamocarb (5-50,000 µg/mL; 10 min-1 h) hydrochloride inhibited zoospore motility in P. nicotianae isolates, with EC50 values ranging from 88.1 to 249.8 µg/mL and complete cessation of motility within 10 min at 5,000 µg/mL or above[2].
Propamocarb (5-50,000 µg/mL; 2 days) hydrochloride inhibited zoospore germination in P. nicotianae isolates, with EC50 values ranging from 1.9 to 184.6 µg/mL across the nine isolates[2].
Propamocarb (0.1-50 µg/mL; 7 days) hydrochloride slightly affected cell viability and moderately reduced mitochondrial function, glucose consumption, and intracellular ATP in primary rat cortical neurons, with these effects being strongly mitigated or completely prevented by pyruvate and acetylcysteine supplementation; the compound showed no direct effect on GSH levels within 1 h[5].
Propamocarb hydrochloride controls late blight in detached potato leaves incited by P. infestans with EC90 values ranging from 72 to 122 ppm, with no significant difference between Metalaxyl (HY-B0843)-sensitive and metalaxyl-resistant isolates[6].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
Chronic exposure of C57BL/6J mice to Propamocarb (1-10 mg/L; p.o.; in drinking water; continuously for 10 weeks) hydrochloride induces bile acid metabolic disorder, with significant increases in hepatic and serous BAs at the 10 mg/L dose, and increases the levels of the cardiovascular disease risk factor trimethylamine[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:ICR mice (Male, 5 weeks old)[3]
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Dosage:3, 30, and 300 mg/L (0.5, 5, and 50 mg/kg bw/day)
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Administration:p.o.; ad libitum; 28 days
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Result:Decreased hepatic TG levels to 75.8% in the 30 mg/L group and 69.7% in the 300 mg/L group.
Significantly decreased mRNA levels of Fatp2 in all treatment groups, Fabp2 in the 30 mg/L group, Acox in all groups, and Scd1 in all groups.
Significantly up-regulated colonic LPL transcription in the 30 and 300 mg/L groups and increased colonic LPL protein in the 3 and 300 mg/L groups.
Decreased colonic GPR41 transcription in all treated groups.
Altered 20 fecal metabolites in the 300 mg/L group, including increased propionate, isobutyrate, total bile acids, taurine, choline, ethanolamine, and trimethylamine, and decreased succinate, trehalose, β-galactose, glycerol, and lactate.
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Animal Model:C57BL/6J mice (Male, 5-week-old)[4]
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Dosage:1 mg/L; 3 mg/L; 10 mg/L
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Administration:p.o.; in drinking water; continuously for 10 weeks
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Result:Increased total hepatic bile acids (BAs) by 29.8% (10.9 mmol/g vs. 8.4 mmol/g) at 10 mg/L.
Down-regulated mRNA levels of Cyp7a1, Cyp8b1, Cyp27a1, and BA transporters Abcb11, Ntcp, Mrp2, and Mrp3 in the liver.
Decreased FXR expression considerably at 10 mg/L.
Decreased Lbabp mRNA level significantly in the ileum at 3 and 10 mg/L.
Increased serum levels of TCA (64.70 vs. 46.30 nmol/L), UDCA (25.81 vs. 12.80 nmol/L), TβMCA (86.4 vs. 42.79 nmol/L), TωMCA (140.60 vs. 92.33 nmol/L), βMCA (298.48 vs. 159.77 nmol/L), and ωMCA (212.62 vs. 148.17 nmol/L) at 10 mg/L.
Decreased expression of genes related to fatty acid translation, β-oxidation, and TG synthesis significantly at 10 mg/L.
Increased hepatic FMO3 protein levels significantly at 1, 3, and 10 mg/L.
Increased cardiac NO levels significantly at 10 mg/L.
Decreased cardiac NOS activities significantly at 3 and 10 mg/L.
Increased transcriptional levels of NF-κB in the heart significantly at 3 and 10 mg/L.
Increased fecal trimethylamine (TMA) significantly at 10 mg/L.
Chemical Information
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CAS No. 25606-41-1
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Appearance Oil
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Molecular Weight 224.73
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Formula C9H21ClN2O2
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Color White to off-white
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SMILES
O=C(NCCCN(C)C)OCCC.Cl
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Pure form -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (444.98 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (282 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[2]. Hu J, et al. Effects of Propamocarb Hydrochloride on Mycelial Growth, Sporulation, and Infection by Phytophthora nicotianae Isolates from Virginia Nurseries. Plant disease. 2007 Apr;91(4):414-420. [Content Brief]
[3]. Wu S, et al. Exposure to the fungicide propamocarb causes gut microbiota dysbiosis and metabolic disorder in mice. Environmental pollution (Barking, Essex : 1987). 2018 Jun;237:775-783. [Content Brief]
[4]. Wu S, et al. Chronic exposure to fungicide propamocarb induces bile acid metabolic disorder and increases trimethylamine in C57BL/6J mice. Sci Total Environ. 2018 Nov 15;642:341-348. [Content Brief]
[5]. Schmuck G, et al. Effects of the carbamates fenoxycarb, propamocarb and propoxur on energy supply, glucose utilization and SH-groups in neurons. Archives of toxicology. 2004 Jun;78(6):330-7. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 4.4498 mL | 22.2489 mL | 44.4978 mL | 111.2446 mL |
| 5 mM | 0.8900 mL | 4.4498 mL | 8.8996 mL | 22.2489 mL | |
| 10 mM | 0.4450 mL | 2.2249 mL | 4.4498 mL | 11.1245 mL | |
| 15 mM | 0.2967 mL | 1.4833 mL | 2.9665 mL | 7.4163 mL | |
| 20 mM | 0.2225 mL | 1.1124 mL | 2.2249 mL | 5.5622 mL | |
| 25 mM | 0.1780 mL | 0.8900 mL | 1.7799 mL | 4.4498 mL | |
| 30 mM | 0.1483 mL | 0.7416 mL | 1.4833 mL | 3.7082 mL | |
| 40 mM | 0.1112 mL | 0.5562 mL | 1.1124 mL | 2.7811 mL | |
| 50 mM | 0.0890 mL | 0.4450 mL | 0.8900 mL | 2.2249 mL | |
| 60 mM | 0.0742 mL | 0.3708 mL | 0.7416 mL | 1.8541 mL | |
| 80 mM | 0.0556 mL | 0.2781 mL | 0.5562 mL | 1.3906 mL | |
| 100 mM | 0.0445 mL | 0.2225 mL | 0.4450 mL | 1.1124 mL |
Propamocarb hydrochloride Related Classifications
HY-W726127 Related Classifications
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Keywords
- Propamocarb
- 25606-41-1
- Fungal
- Biochemical Assay Reagents
- oomycete membrane biosynthesis inhibitor
- gut microbiota dysbiosis
- cardiovascular disease
- cucumber fruit peel
- late blight of potato
- Phytophthora nicotianae
- phenylpropanoid pathway
- phenylalanine ammonia-lyase (PAL) inducer
- lignin synthesis
- Phytophthora infestans
- Inhibitor
- inhibitor
- inhibit