Higenamine 4'-O-β-d-glucoside in the lotus plumule induces glucose uptake of L6 cells through β2-adrenergic receptor

  • Bioorg Med Chem. 2015 Jul 1;23(13):3317-21. doi: 10.1016/j.bmc.2015.04.054.
Eisuke Kato  1 ,  Yosuke Inagaki  2 ,  Jun Kawabata  3
Affiliations
  • 1. Laboratory of Food Biochemistry, Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Kita-ku, Sapporo, Hokkaido 060-8589, Japan. Electronic address: [email protected].
  • 2. Nippon Supplement, Inc., Applause Tower 18F, 19-19, Chayamachi, Kita-ku, Osaka 530-0013, Japan.
  • 3. Laboratory of Food Biochemistry, Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Kita-ku, Sapporo, Hokkaido 060-8589, Japan.
PMID: 25943853 DOI: 10.1016/j.bmc.2015.04.054
Abstract

Hypoglycemic effect is an efficient means to modulate elevated blood glucose levels in patients with diabetes. We found that the extract of lotus plumule (the germ of Nelumbo nucifera Gaertn. seed) showed potent glucose uptake enhancement activity against L6 myotubes, which results in a hypoglycemic effect. This activity was further investigated, and an active constituent was identified as a single bioactive compound, higenamine 4'-O-β-d-glucoside. Mechanistic studies employing phosphatidylinositol 3-kinase (PI3K) inhibitor, AMP-activated protein kinase (AMPK) inhibitor, or Adrenergic Receptor antagonist showed that the compound induced its activity through β2-adrenergic receptor. Patients with type II diabetes mellitus frequently develop Insulin resistance. Owing to the differences between the mechanism of action of Insulin and of the isolated compound, the compound or lotus plumule itself may have the possibility of modulating blood glucose levels in insulin-resistant patients effectively.

Keywords
Diabetes mellitus; Glucose uptake; Hypoglycemic effect; Nelumbo nucifera Gaertn.; β2-Adrenergic receptor agonist.
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