Anti-inflammatory effect of a novel synthetic compound 1-((4-fluorophenyl)thio)isoquinoline in RAW264.7 macrophages and a zebrafish model

  • Fish Shellfish Immunol. 2019 Apr;87:395-400. doi: 10.1016/j.fsi.2019.01.030.
Geum Ran Kim  1 ,  Jung Yoon Yang  2 ,  Kyu-Seok Hwang  2 ,  Seong Soon Kim  2 ,  Jin Sil Chae  2 ,  Hyemin Kan  2 ,  Jin Hee Ahn  3 ,  Won Mi Lee  3 ,  Se Hwan Ahn  3 ,  Yong-Moon Lee  4 ,  Myung Ae Bae  5 ,  Dae-Seop Shin  6
Affiliations
  • 1. Bio Platform Technology Research Center, Korea Research Institute of Chemical Technology, Daejeon, 34114, South Korea; College of Pharmacy, Chungbuk National University, Cheongju, 28160, South Korea.
  • 2. Bio Platform Technology Research Center, Korea Research Institute of Chemical Technology, Daejeon, 34114, South Korea.
  • 3. Department of Chemistry, Gwangju Institute of Science and Technology, Gwangju, 61005, South Korea.
  • 4. College of Pharmacy, Chungbuk National University, Cheongju, 28160, South Korea.
  • 5. Bio Platform Technology Research Center, Korea Research Institute of Chemical Technology, Daejeon, 34114, South Korea; Department of Medicinal Chemistry and Pharmacology, University of Science & Technology, Daejeon, 34114, South Korea. Electronic address: [email protected].
  • 6. Bio Platform Technology Research Center, Korea Research Institute of Chemical Technology, Daejeon, 34114, South Korea. Electronic address: [email protected].
PMID: 30685466 DOI: 10.1016/j.fsi.2019.01.030
Abstract

The compound, 1-((4-fluorophenyl)thio)isoquinoline (FPTQ), is a synthetic isoquinoline derivative. To test the anti-inflammatory effect of FPTQ, we used neutrophil-specific transgenic zebrafish Tg(mpx::EGFP)i114 line and lipopolysaccharide (LPS)-stimulated RAW264.7 cells. We also used two different methods, involving tail transection and LPS stimulation in the zebrafish model. Neutrophils translocation in the zebrafish tail-transected model was inhibited by FPTQ. Neutrophil aggregation was also inhibited by FPTQ in the LPS-stimulated zebrafish model. Decreased mRNA expression of the pro-inflammatory cytokine genes, interleukin-1β (il-1β) and interleukin-6 (IL-6), was found in zebrafish larvae injected with FPTQ. Additionally, production of nitric oxide was inhibited by FPTQ in RAW264.7 macrophage cells treated with LPS. Moreover, the mRNA expression of Il-1β and IL-6 suppressed by FPTQ treatment in RAW264.7 macrophage cells, and an enzyme Immunoassay showed that FPTQ suppressed the secretion of IL-1β and IL-6 in RAW264.7 cells. These results demonstrate that FPTQ reduced inflammatory responses and, therefore, suggest that it may be effective as an anti-inflammatory agent.

Keywords
Anti-Inflammation; Neutrophil; RAW264.7; Zebrafish.
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