Metabolite Profiling and Quantitation of Cucurbitacins in Cucurbitaceae Plants by Liquid Chromatography coupled to Tandem Mass Spectrometry

  • Sci Rep. 2019 Nov 5;9(1):15992. doi: 10.1038/s41598-019-52404-1.
Faraz Ul Haq  1 ,  Arslan Ali  2 ,  Muhammad Noman Khan  1 ,  Syed Muhammad Zaki Shah  1 ,  Ram Chandra Kandel  3 ,  Nudrat Aziz  1 ,  Achyut Adhikari  3 ,  M Iqbal Choudhary  1  2  4 ,  Atta- Ur-Rahman  1  2 ,  Hesham R El-Seedi  5  6 ,  Syed Ghulam Musharraf  7  8
Affiliations
  • 1. H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi, 75270, Pakistan.
  • 2. Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, Karachi, 75270, Pakistan.
  • 3. Central Department of Chemistry, Tribhuvan University, Kirtipur, Kathmandu, Nepal.
  • 4. Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah, 21452, Saudi Arabia.
  • 5. Pharmacognosoy Group, Department of Medicinal Chemistry, Uppsala University, Biomedical Centre, Box 574, SE-75 123, Uppsala, Sweden. [email protected].
  • 6. Alrayan Medical College, Medina, 42541, Kingdom of Saudi Arabia. [email protected].
  • 7. H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi, 75270, Pakistan. [email protected].
  • 8. Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, Karachi, 75270, Pakistan. [email protected].
PMID: 31690753 DOI: 10.1038/s41598-019-52404-1
Abstract

Cucurbitaceae is an important plant family because many of its species are consumed as food, and used in herbal medicines, cosmetics, etc. It comprises annual vines and is rich in various bioactive principles which include the cucurbitacins. These steroidal natural products, derived from the triterpene cucurbitane, are mainly the bitter principles of the family Cucurbitaceae. Their biological activities include anti-inflammatory, hepatoprotective, and anti-cancer activities. A total of 10 species belonging to 6 genera of the Cucurbitaceae family along with Cissampelos pareira (Menispermaceae) were included in this study. A comprehensive profiling of certain natural products was developed using HPLC-QTOF-MS/MS analysis and a distribution profile of several major natural products in this family was obtained. A total of 51 natural products were detected in both positive and negative ionization modes, based on accurate masses and fragmentation patterns. Along with this, quantitation of four bioactive cucurbitacins, found in various important Plants of the Cucurbitaceae family, was carried out using multiple reaction monitoring (MRM) approach on an ion trap mass spectrometer. Cucurbitacin Q was found to be the most abundant in C. pareira, while Citrullus colocynthis contained all four cucurbitacins in abundant quantities. The developed quantitation method is simple, rapid, and reproducible.

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