Anti-inflammatory isocoumarins from the bark of Fraxinus chinensis subsp. rhynchophylla

  • Nat Prod Res. 2021 Nov;35(22):4380-4387. doi: 10.1080/14786419.2020.1715401.
Beom Zoo Lee  1  2 ,  Chan-Sik Kim  3 ,  Soon-Kyu Jeong  2 ,  Sulhae Lee  2 ,  Ik Soo Lee  3 ,  KwangWon Hong  1
Affiliations
  • 1. Department of Food Science and Biotechnology, College of Life Science and Biotechnology, Dongguk University, Goyang, Republic of Korea.
  • 2. Chemland Co., Ltd., Gunpo, Republic of Korea.
  • 3. Herbal Medicine Research Division, Korea Institute of Oriental Medicine, Daejeon, Republic of Korea.
PMID: 31960706 DOI: 10.1080/14786419.2020.1715401
Abstract

A new isocoumarin (1) named fraxicoumarin was isolated from the bark of Fraxinus chinensis subsp. rhynchophylla along with three known compounds (2-4). The structure of the new compound was established by extensive spectroscopic studies and chemical evidence. The anti-inflammatory effects of the isolated compounds (1-4) on lipopolysaccharide (LPS)-induced RAW 264.7 macrophage cells were evaluated in vitro. Of the compounds tested, compounds 1 and 3 inhibited LPS-induced nitric oxide (NO) production in RAW 264.7 cells. Consistent with these findings, they also suppressed LPS-induced expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) at the protein level in RAW 264.7 cells.

Keywords
Fraxinus chinensis subsp. rhynchophylla; anti-inflammation; cyclooxygenase-2; inducible nitric oxide synthase; isocoumarins; nitric oxide.
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