12-OAHSA is a component of olive oil and mitigates obesity-induced inflammation

  • J Nutr Biochem. 2022 Dec:110:109127. doi: 10.1016/j.jnutbio.2022.109127.
Knowledge Mudhibadhi Moyo  1 ,  Jieun Choi  2 ,  Jiyeon Chang  3 ,  Shindy Soedono  3 ,  Dan Vo Hoang Nguyet  3 ,  Yu-Ri Song  1 ,  Soo Jin Park  2 ,  Gwang-Woong Go  4 ,  Do Yup Lee  5 ,  Kae Won Cho  6
Affiliations
  • 1. Soonchunhyang Institute of Medi-bio Science (SIMS), Soonchunhyang University, Cheonan, Republic of Korea.
  • 2. Department of Agricultural Biotechnology, Center for Food and Bioconvergence, and Research Institute for Agriculture and Life Sciences, CALS, Seoul National University, Seoul, Republic of Korea.
  • 3. Department of Integrated Biomedical Science, Soonchunhyang University, Cheonan, Republic of Korea.
  • 4. Department of Food and Nutrition, Hanyang University, Seoul, Republic of Korea.
  • 5. Department of Agricultural Biotechnology, Center for Food and Bioconvergence, and Research Institute for Agriculture and Life Sciences, CALS, Seoul National University, Seoul, Republic of Korea. Electronic address: [email protected].
  • 6. Soonchunhyang Institute of Medi-bio Science (SIMS), Soonchunhyang University, Cheonan, Republic of Korea; Department of Integrated Biomedical Science, Soonchunhyang University, Cheonan, Republic of Korea. Electronic address: [email protected].
PMID: 35977667 DOI: 10.1016/j.jnutbio.2022.109127
Abstract

Fatty acid esters of hydroxyl fatty acids (FAHFAs) are a new family of endogenous lipids that exert anti-inflammatory action. Among the various FAHFA isomers, the dietary source of oleic acid-hydroxy stearic acid (OAHSA) and its anti-inflammatory functions are poorly understood. This study investigated the composition of OAHSA isomers in dietary oils and the impact of 12-OAHSA on obesity-induced inflammation. Liquid chromatography with tandem mass spectrometry analysis revealed that various dietary oils, including fish oil, corn oil, palm oil, soybean oil, and olive oil, present a wide variation in OAHSA profiles and amounts. The highest amounts of total OAHSAs are present in olive oil including 12-OAHSA. Compared to vehicle-treated obese mice, administration of 12-OAHSA significantly improved glucose homeostasis, independent of body weight. 12-OAHSA-treated mice displayed significantly reduced accumulation of CD11c+ adipose tissue macrophages, and CD4+/CD8+ adipose tissue T lymphocytes. Concomitantly, the expression of pro-inflammatory cytokine genes and the nuclear factor kappa-light-chain-enhancer of activated B cells signaling pathway were significantly decreased in the 12-OAHSA-treated adipose tissue, while the expression of the anti-inflammatory gene Il10 was markedly increased. Moreover, in vitro Cell Culture experiments showed that 12-OAHSA significantly inhibited the lipopolysaccharides-induced inflammatory response in macrophages by suppressing the nuclear factor kappa-light-chain-enhancer of activated B cells signaling pathway. Collectively, these results indicated that 12-OAHSA, as a component of olive oil, mitigates obesity-induced Insulin resistance by regulating AT inflammation. Therefore, 12-OAHSA could be used as a novel nutritional intervention against obesity-associated metabolic dysregulation.

Keywords
12-OAHSA; Adipose tissue inflammation; FAHFA; LC-MS/MS; Olive oil.
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