Pyridoxal hydrochloride
Based on 1 publication(s) in Google Scholar
Pyridoxal hydrochloride, the hydrochloride form of Pyridoxal (HY-107469), is a newly developed neuroprotective agent. Pyridoxal hydrochloride is one of the major forms of vitamin B6. Pyridoxal hydrochloride is solvolyzed in methanol to form pyridoxal monomethylacetal. Pyridoxal is phosphorylated by pyridoxal kinase to Pyridoxal phosphate (HY-B1744). Pyridoxal is oxidized by the liver to 4-Pyridoxic acid (HY-113493) which is excreted in the urine. Pyridoxal hydrochloride is proming for research of carpal tunnel syndrome (CTS).
For research use only. We do not sell to patients.
- Purity: 99.90%
- CAS No.: 65-22-5
- Formula: C8H10ClNO3
- Molecular Weight:203.62
-
Storage:
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Pyridoxal hydrochloride
MoreAll Endogenous Metabolite Isoforms
More
Biological Activity
Pyridoxal hydrochloride (5 μM) has an increasing effect on the activity of tyrosine decarboxylase[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 65-22-5
-
Appearance Solid
-
Molecular Weight 203.62
-
Formula C8H10ClNO3
-
Color White to light yellow
-
SMILES
O=CC1=C(C=NC(C)=C1O)CO.Cl
-
Structure Classification
-
Initial Source
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
Publications (1)
-
Journal Impact Factor
-
Most Recent
-
Dev Cell
2025 Nov 27:S1534-5807(25)00691-4. PMID: 41314217
Solvent & Solubility
DMSO : 50 mg/mL (245.55 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 33.33 mg/mL (163.68 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (24.55 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (24.55 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (491.10 mM); Clear solution; Need ultrasonic
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
-
Data Sheet (276 KB)
-
SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
-
Handling Instructions (2659 KB)
References
[1]. Vrolijk MF, et al. The vitamin B6 paradox: Supplementation with high concentrations of pyridoxine leads to decreased vitamin B6 function[J]. Toxicol In Vitro. 2017 Oct;44:206-212. [Content Brief]
[2]. Aufiero E, et al. Pyridoxine hydrochloride treatment of carpal tunnel syndrome: a review[J]. Nutr Rev. 2004 Mar;62(3):96-104. [Content Brief]
[4]. Vrolijk MF, et al. The vitamin B6 paradox: Supplementation with high concentrations of pyridoxine leads to decreased vitamin B6 function. Toxicol In Vitro. 2017 Oct;44:206-212. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 4.9110 mL | 24.5549 mL | 49.1099 mL | 122.7747 mL |
| 5 mM | 0.9822 mL | 4.9110 mL | 9.8220 mL | 24.5549 mL | |
| 10 mM | 0.4911 mL | 2.4555 mL | 4.9110 mL | 12.2775 mL | |
| 15 mM | 0.3274 mL | 1.6370 mL | 3.2740 mL | 8.1850 mL | |
| 20 mM | 0.2455 mL | 1.2277 mL | 2.4555 mL | 6.1387 mL | |
| 25 mM | 0.1964 mL | 0.9822 mL | 1.9644 mL | 4.9110 mL | |
| 30 mM | 0.1637 mL | 0.8185 mL | 1.6370 mL | 4.0925 mL | |
| 40 mM | 0.1228 mL | 0.6139 mL | 1.2277 mL | 3.0694 mL | |
| 50 mM | 0.0982 mL | 0.4911 mL | 0.9822 mL | 2.4555 mL | |
| 60 mM | 0.0818 mL | 0.4092 mL | 0.8185 mL | 2.0462 mL | |
| 80 mM | 0.0614 mL | 0.3069 mL | 0.6139 mL | 1.5347 mL | |
| 100 mM | 0.0491 mL | 0.2455 mL | 0.4911 mL | 1.2277 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Pyridoxal hydrochloride Related Classifications
HY-W027446 Related Classifications
Powered by Bioz