QEQLERALNSS TFA
QEQLERALNSS TFA is a helix B surface peptide (HBSP) derived from erythropoietin with tissue protective activities. QEQLERALNSS TFA protects cardiomyocytes from apoptosis.
For research use only. We do not sell to patients.
- Formula: C53H88F3N17O23
- Molecular Weight:1388.36
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
QEQLERALNSS (2.5 and 25 ng/mL; 12 h) clearly decreases TNF-α-induced apoptosis of cardiomyocytes[1].
QEQLERALNSS (2.5 ng/mL; 5 min) activates critical signaling pathways of cell survival, including Akt, ERK1/2, and STAT3 in cardiomyocytes[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Cardiomyocytes
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Concentration:2.5 and 25 ng/mL
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Incubation Time:12 h
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Result:Decreased the number of apoptotic cells (≈80% pretreatment, ≈60% without pretreatment) induced by TNF-α (50 pg/mL).
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Cell Line:Cardiomyocytes
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Concentration:2.5 ng/mL
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Incubation Time:5 min
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Result:Rapidly activated Akt, ERK1/2, and STAT3 with a peak at 1 min after incubation.
QEQLERALNSS (30 μg/kg; s.c.; three times a week for 6 month) results in a significant decrease in the number of apoptotic cardiomyocytes in the cardiac sections compared with the control in DCM hamsters[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Male Sprague-Dawley rats, renal IR injury model[2]
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Dosage:8 nmol/kg
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Administration:Intraperitoneal injection, once a day for 2 weeks
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Result:Decreased the level of blood urea nitrogen and tubulointerstitial damage induced by Cyclosporin A (HY-B0579). Reduced apoptotic cells, myeloperoxidase (MPO)+ cells and active caspase-3+ cells.
Chemical Information
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Molecular Weight 1388.36
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Formula C53H88F3N17O23
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Sequence Shortening
QEQLERALNSS
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent & Solubility
H2O
Peptide Solubility and Storage Guidelines:
1. Calculate the length of the peptide.
2. Calculate the overall charge of the entire peptide according to the following table:
| Contents | Assign value | |
|---|---|---|
| Acidic amino acid | Asp (D), Glu (E), and the C-terminal -COOH. | -1 |
| Basic amino acid | Arg (R), Lys (K), His (H), and the N-terminal -NH2 | +1 |
| Neutral amino acid | Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) | 0 |
3. Recommended solution:
| Overall charge of peptide | Details |
|---|---|
| Negative (<0) |
1. Try to dissolve the peptide in water first. 2. If water fails, add NH4OH (<50 μL). 3. If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide. |
| Positive (>0) |
1. Try to dissolve the peptide in water first. 2. If water fails, try dissolving the peptide in a 10%-30% acetic acid solution. 3. If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO. |
| Zero (=0) |
1. Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first. 2. For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration. |
Purity & Documentation
References
[1]. Ueba H, et al. Cardioprotection by a nonerythropoietic, tissue-protective peptide mimicking the 3D structure of erythropoietin. Proc Natl Acad Sci U S A. 2010 Aug 10;107(32):14357-62. [Content Brief]
[2]. Wu Y, et al. Protective effects of HBSP on ischemia reperfusion and cyclosporine a induced renal injury. Clin Dev Immunol. 2013;2013:758159. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
QEQLERALNSS TFA Related Classifications
HY-P3868A Related Classifications
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