(Rac)-EGC-M5
(Rac)-EGC-M5 is an orally active metabolite of green tea Catechin (EGC) (HY-N0898). (Rac)-EGC-M5 enhances the activity of CD4+ T cells. (Rac)-EGC-M5 boosts the cytotoxic activity of NK cells in vivo. (Rac)-EGC-M5 can be used in cancer-related research.
For research use only. We do not sell to patients.
- CAS No.: 1299471-83-2
- Formula: C11H12O4
- Molecular Weight:208.21
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
(Rac)-EGC-M5 (10 μM; 72 h) significantly increases relative ATP levels in isolated mouse splenic CD4+ T cells after 72 h incubation, enhancing CD4+ T cell activation[1].
(Rac)-EGC-M5 (5-50 μM; 72 h) significantly upregulates PHA-induced IFN-γ production in isolated mouse splenocytes, while having no significant effect on PHA-induced IL-2 levels[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:Mouse spleen cells
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Concentration:10, 25 and 50 μM
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Incubation Time:72 h
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Result:Significantly upregulated the secretion of IFN-γ induced by PHA.
Was no significant change in the level of IL-2.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:BALB/c mice (male, 8-10 weeks old)[1]
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Dosage:10 mg/kg
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Administration:p.o.; daily; 14 days
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Result:Increased splenic NK cell cytotoxicity against YAC-1 target cells to ~330% of control at an effector:target ratio of 20:1 and ~350% of control at an effector:target ratio of 40:1.
Increased the percentage of granzyme B+ NK cells to ~0.42%.
Showed no significant effect on populations of perforin+ NK cells, IFN-γ+ NK cells, IFN-γ+ CD8+ cells, helper T cells, cytotoxic T cells, B cells, dendritic cells, macrophages, total NK cells, Th1 cells, Th2 cells, Th17 cells, or Treg cells.
Chemical Information
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CAS No. 1299471-83-2
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Molecular Weight 208.21
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Formula C11H12O4
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SMILES
O=C1OC(CC1)CC2=CC(O)=CC(O)=C2
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
(Rac)-EGC-M5 Related Classifications
HY-186205 Related Classifications
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