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Enolase 1/ENO1 Protein, Human (His, solution)

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Based on 1 Customer Validation

The ENO1 protein converts 2-phosphoglycerate to phosphoenolpyruvate and is involved in growth control, hypoxia tolerance, and allergic responses. It acts as a receptor and activator of plasminogen, stimulating immunoglobulin production. ENO1 binds to the myc promoter as a transcriptional repressor and may function as a tumor suppressor. Enolase 1/ENO1 Protein, Human (His, solution) is the recombinant human-derived ENO1, expressed by E. coli , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Experimental Validation Results for Enolase 1/ENO1 Protein, Human (His, solution)
  • Enolase 1/ENO1 Protein, Human (His, solution) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • References
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

The ENO1 protein converts 2-phosphoglycerate to phosphoenolpyruvate and is involved in growth control, hypoxia tolerance, and allergic responses. It acts as a receptor and activator of plasminogen, stimulating immunoglobulin production. ENO1 binds to the myc promoter as a transcriptional repressor and may function as a tumor suppressor. Enolase 1/ENO1 Protein, Human (His, solution) is the recombinant human-derived ENO1, expressed by E. coli , with C-His labeled tag.

Background

α-enolase is expressed on the surface of several cell types, where it acts as a plasminogen receptor, concentrating proteolytic plasmin activity on the cell surface. Differential expression of α-enolase has been related to several pathologies, such as cancer, Alzheimer's disease, and rheumatoid arthritis, among others. α-Enolase can be considered as a marker of pathological stress in a high number of diseases, performing several of its multiple functions, mainly as plasminogen receptor[1].

Verified Bioactivity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag C-6*His
  • Accession

    P06733-1 (M1-K434)

  • Gene ID
    2023  [NCBI]
  • Molecular Construction
    • N-term
    • ENO1 (M1-K434)
      Accession # P06733-1
    • 6*His
    • C-term
  • Protein Length

    Full Length of Isoform-1

  • Synonyms

    ENO1; Enolase 1, (Alpha); Prev. ENO1L1; NNE; Prev. MPB1; Epididymis Secretory Protein Li 17; Prev. ENO1-IT1; C-Myc Promoter-Binding Protein-1; 2-Phospho-D-Glycerate Hydro-Lyase; MYC Promoter-Binding Protein 1; Phosphopyruvate Hydratase; C-Myc Promoter-Bin

  • AA Sequence

    MSILKIHAREIFDSRGNPTVEVDLFTSKGLFRAAVPSGASTGIYEALELRDNDKTRYMGKGVSKAVEHINKTIAPALVSKKLNVTEQEKIDKLMIEMDGTENKSKFGANAILGVSLAVCKAGAVEKGVPLYRHIADLAGNSEVILPVPAFNVINGGSHAGNKLAMQEFMILPVGAANFREAMRIGAEVYHNLKNVIKEKYGKDATNVGDEGGFAPNILENKEGLELLKTAIGKAGYTDKVVIGMDVAASEFFRSGKYDLDFKSPDDPSRYISPDQLADLYKSFIKDYPVVSIEDPFDQDDWGAWQKFTASAGIQVVGDDLTVTNPKRIAKAVNEKSCNCLLLKVNQIGSVTESLQACKLAQANGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLLRIEEELGSKAKFAGRNFRNPLAK

  • Predicted Molecular Mass

    48 kDa

  • Molecular Weight

    Approximately 45-55 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of 20 mM Tris-HCl, 250 mM NaCl, 1 mM MgSO4, 20% Glycerol, 5% trehalose, 5% Mannitol, 0.02% Tween80, pH 7.5.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

References

[1]. Angels Díaz-Ramos, et al. α-Enolase, a multifunctional protein: its role on pathophysiological situations. J Biomed Biotechnol. 2012;2012:156795. [Content Brief]

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • Enolase 1/ENO1 Protein, Human (His, solution) Product Image
    1/16
  • Experimental Validation Results for Enolase 1/ENO1 Protein, Human (His, solution)
  • Enolase 1/ENO1 Protein, Human (His, solution) Product Image