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ART1 Protein, Cynomolgus (HEK293, His)

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Based on 1 Customer Validation

ART1 Protein is an arginine-specific ADP-ribosyltransferase. ART1 is involved in the regulation of a diverse array of pathophysiological processes, including proliferation, invasion, apoptosis, autophagy and angiogenesis of colorectal cancer (CRC) cells. ART1 plays a crucial role in the elevation of glucose consumption in CT26 cells and may regulate GLUT1-dependent glycolysis in CRC via the PI3K/AKT/HIF1α pathway. ART1 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived ART1 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Cynomolgus
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for ART1 Protein, Cynomolgus (HEK293, His)
  • Experimental Validation Results for ART1 Protein, Cynomolgus (HEK293, His)
  • ART1 Protein, Cynomolgus (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

ART1 Protein is an arginine-specific ADP-ribosyltransferase. ART1 is involved in the regulation of a diverse array of pathophysiological processes, including proliferation, invasion, apoptosis, autophagy and angiogenesis of colorectal cancer (CRC) cells. ART1 plays a crucial role in the elevation of glucose consumption in CT26 cells and may regulate GLUT1-dependent glycolysis in CRC via the PI3K/AKT/HIF1α pathway. ART1 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived ART1 protein, expressed by HEK293 , with C-His labeled tag.

Background

NAD(P)(+)--arginine ADP-ribosyltransferase (ART1), an arginine-specific ADP-ribosyltransferase, catalyzes the transfer of ADP-ribose moiety to arginine residues on an acceptor protein using nicotinamide adenine dinucleotide (NAD+) as a substrate, thus eliciting changes in the activities and functions of the acceptor proteins. ART1 is involved in the regulation of a diverse array of pathophysiological processes, including proliferation, invasion, apoptosis, autophagy and angiogenesis of colorectal cancer (CRC) cells. ART1 plays a crucial role in the elevation of glucose consumption in CT26 cells and may regulate GLUT1-dependent glycolysis in CRC via the PI3K/AKT/HIF1α pathway. In addition, ART1 plays a role in glycolysis and energy metabolism under high glucose conditions and promotes angiogenesis in colorectal cancer cells through the PI3K/AKT pathway[1].

Verified Bioactivity

Measured in a cell proliferation assay using A549 cells. The ED50 for this effect is 17.43 ng/mL. Corresponding to a specific activity is 5.737×10^4 Unit/mg.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - Cell-Based Assay

    Bioactivity - Cell-Based Assay

    Measured in a cell proliferation assay using A549 cells.The ED50 for this effect is 17.43 ng/mL. Corresponding to a specific activity is 5.737×104 Unit/mg.

Technical Parameters

  • Species Cynomolgus
  • Source HEK293
  • Tag C-6*His
  • Accession

    A0A2K5WCR3/XP_005579037 (Q23-N294)

  • Gene ID
    102142531  [NCBI]
  • Molecular Construction
    • N-term
    • ART1 (Q23-N294)
      Accession # A0A2K5WCR3/XP_005579037
    • His
    • C-term
  • Protein Length

    Partial

  • Synonyms

    ART1; ADP-Ribosyltransferase C2 And C3 Toxin-Like 1; ADP-Ribosyltransferase 1; Mono(ADP-Ribosyl)Transferase 1; ARTC1; ADP-Ribosyltransferase 2; CD296; CD296 Antigen; ART2; RT6; GPI-Linked NAD(P)(+)--Arginine ADP-Ribosyltransferase 1

  • AA Sequence

    QSHPITRRDLFSQEMPLDMALASFDDQYAGCAAAMTAALPDLNHTEFQANKVYADGWTLASSQWQERQAWGPEWSLSPTRPPPPPLGFRDEHGVALLAYTANSPLHKEFNAAVREAGRSRAHYLHHFSFKTLHFLLTEALQLLGRGQRPPRCHQVFRGVHGLHFRPAGPGATVRLGGFASASLKNVAAQQFGEDTFFGIWTCLGAPIKGYSFFPGEEEVLIPPFETFQVINASRPAQGPARIYLRALGKRSTYNCEYIKDKKCKSGPCHLDN

  • Molecular Weight

    Approximately 33-34 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured in a cell proliferation assay using A549 cells.The ED50 for this effect is 17.43 ng/mL. Corresponding to a specific activity is 5.737×104 Unit/mg.

  • ART1 Protein, Cynomolgus (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for ART1 Protein, Cynomolgus (HEK293, His)
  • Experimental Validation Results for ART1 Protein, Cynomolgus (HEK293, His)
  • ART1 Protein, Cynomolgus (HEK293, His) Product Image