BRSK1 Protein, Human (Active, sf9, His-GST)
The BRSK1 protein is a critical serine/threonine protein kinase that regulates cortical neuronal polarization and centrosome duplication. BRSK1 is phosphorylated and activated by STK11/LKB1, affecting neuronal polarization through MAPT/TAU phosphorylation (“Thr-529” and “Ser-579”) and WEE1 phosphorylation (“Ser-642”). BRSK1 Protein, Human (sf9, His-GST) is the recombinant human-derived BRSK1 protein, expressed by sf9 insect cells , with N-6*His and N-GST labeled tag.
- Species: Human
- Source: Sf9 insect cells
-
Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Technical Parameters
-
Product Properties
- Help & FAQs
Biological Activity
Description
The BRSK1 protein is a critical serine/threonine protein kinase that regulates cortical neuronal polarization and centrosome duplication. BRSK1 is phosphorylated and activated by STK11/LKB1, affecting neuronal polarization through MAPT/TAU phosphorylation (“Thr-529” and “Ser-579”) and WEE1 phosphorylation (“Ser-642”). BRSK1 Protein, Human (sf9, His-GST) is the recombinant human-derived BRSK1 protein, expressed by sf9 insect cells , with N-6*His and N-GST labeled tag.
Background
BRSK1 Protein functions as a pivotal serine/threonine-protein kinase crucial for the polarization of neurons and centrosome duplication. Upon phosphorylation and activation by STK11/LKB1, BRSK1 assumes a central role in regulating the polarization of cortical neurons, likely through the phosphorylation of microtubule-associated proteins such as MAPT/TAU at 'Thr-529' and 'Ser-579.' Additionally, it participates in neuron polarization by mediating the phosphorylation of WEE1 at 'Ser-642' in postmitotic neurons, leading to the down-regulation of WEE1 activity in polarized neurons. In neurons, BRSK1 localizes to synaptic vesicles and contributes to neurotransmitter release, possibly through the phosphorylation of RIMS1. Furthermore, it acts as a positive regulator of centrosome duplication by phosphorylating gamma-tubulin (TUBG1 and TUBG2) at 'Ser-131,' facilitating the translocation of gamma-tubulin and its associated proteins to the centrosome. BRSK1 also participates in the UV-induced DNA damage checkpoint response, potentially inhibiting CDK1 activity through the phosphorylation and activation of WEE1, along with the inhibition of CDC25B and CDC25C.
Technical Parameters
-
Species Human
-
Source Sf9 insect cells
-
Tag N-6*His;N-GST
-
Accession
Q8TDC3-1 (M1-P778)
-
Gene ID84446
-
Molecular Construction
-
N-term
-
6*His-GST
-
BRSK1 (M1-P778)
Accession # Q8TDC3 -
C-term
-
-
Protein Length
Full Length of Isoform-1
-
Synonyms
BRSK1; Brain-Selective Kinase 1; BR Serine/Threonine Kinase 1; SAD1 Homolog; KIAA1811; HSAD1; SAD-B; Non-Specific Serine/Threonine Protein Kinase; Brain-Specific Serine/Threonine-Protein Kinase 1; Brain Selective Kinase 1; Synapses Of Amphids Defective Ho
-
AA Sequence
MSSGAKEGGGGSPAYHLPHPHPHPPQHAQYVGPYRLEKTLGKGQTGLVKLGVHCITGQKVAIKIVNREKLSESVLMKVEREIAILKLIEHPHVLKLHDVYENKKYLYLVLEHVSGGELFDYLVKKGRLTPKEARKFFRQIVSALDFCHSYSICHRDLKPENLLLDEKNNIRIADFGMASLQVGDSLLETSCGSPHYACPEVIKGEKYDGRRADMWSCGVILFALLVGALPFDDDNLRQLLEKVKRGVFHMPHFIPPDCQSLLRGMIEVEPEKRLSLEQIQKHPWYLGGKHEPDPCLEPAPGRRVAMRSLPSNGELDPDVLESMASLGCFRDRERLHRELRSEEENQEKMIYYLLLDRKERYPSCEDQDLPPRNDVDPPRKRVDSPMLSRHGKRRPERKSMEVLSITDAGGGGSPVPTRRALEMAQHSQRSRSVSGASTGLSSSPLSSPRSPVFSFSPEPGAGDEARGGGSPTSKTQTLPSRGPRGGGAGEQPPPPSARSTPLPGPPGSPRSSGGTPLHSPLHTPRASPTGTPGTTPPPSPGGGVGGAAWRSRLNSIRNSFLGSPRFHRRKMQVPTAEEMSSLTPESSPELAKRSWFGNFISLDKEEQIFLVLKDKPLSSIKADIVHAFLSIPSLSHSVLSQTSFRAEYKASGGPSVFQKPVRFQVDISSSEGPEPSPRRDGSGGGGIYSVTFTLISGPSRRFKRVVETIQAQLLSTHDQPSVQALADEKNGAQTRPAGAPPRSLQPPPGRPDPELSSSPRRGPPKDKKLLATNGTPLP
-
Predicted Molecular Mass
114.2 kDa
-
Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz
