Copy Link Print Information

Cadherin-9/CDH9 Protein, Human (HEK293, His)

Customer Review

Based on 1 Customer Validation

Cadherin-9/CDH9 is a calcium-dependent cell adhesion protein that plays a key role in mediating cell adhesion through preferential homologous interactions, promoting self-association between adjacent cells. This emphasizes its importance in cell adhesion and implies a significant contribution to the sorting of heterogeneous cell types. Cadherin-9/CDH9 Protein, Human (HEK293, His) is the recombinant human-derived Cadherin-9/CDH9 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Cadherin-9/CDH9 Protein, Human (HEK293, His)
  • Experimental Validation Results for Cadherin-9/CDH9 Protein, Human (HEK293, His)
  • Cadherin-9/CDH9 Protein, Human (HEK293, His) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download

To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

Cadherin-9/CDH9 is a calcium-dependent cell adhesion protein that plays a key role in mediating cell adhesion through preferential homologous interactions, promoting self-association between adjacent cells. This emphasizes its importance in cell adhesion and implies a significant contribution to the sorting of heterogeneous cell types. Cadherin-9/CDH9 Protein, Human (HEK293, His) is the recombinant human-derived Cadherin-9/CDH9 protein, expressed by HEK293 , with C-His labeled tag.

Background

Cadherin-9/CDH9 protein, part of the calcium-dependent cell adhesion protein family, plays a pivotal role in mediating cellular adhesion. Operating through preferential homophilic interactions, Cadherin-9/CDH9 facilitates self-binding between adjacent cells. This homophilic cadherin engagement underscores its significance in cell adhesion and implies a substantial contribution to the intricate process of sorting heterogeneous cell types. Through its calcium-dependent adhesion properties, Cadherin-9/CDH9 actively participates in establishing selective connections between cells, thereby influencing the dynamic regulation of cellular interactions and contributing to the organization of tissues.

Verified Bioactivity

Measured by its binding ability in a functional ELISA. When Recombinant Human Cadherin-9 is immobilized at 1 μg/mL can bind Biotinylated Human Cadherin-6. The ED50 for this effect is <6 μg/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 90%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Measured by its binding ability in a functional ELISA. When Recombinant Human Cadherin-9 is immobilized at 1 μg/mL can bind Biotinylated Human Cadherin-6. The ED50 for this effect is 5.36 μg/mL.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-His
  • Accession

    Q9ULB4-1 (G54-A615)

  • Gene ID
    1007  [NCBI]
  • Molecular Construction
    • N-term
    • CDH9 (G54-A615)
      Accession # Q9ULB4-1
    • His
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    CDH9; Cadherin 9, Type 2 (T1-Cadherin); Cadherin 9; CDH9 Protein; Cadherin-9; T1-Cadherin

  • AA Sequence

    GWMWNQFFLLEEYTGTDTQYVGKLHTDQDKGDGNLKYILTGDGAGSLFVIDENTGDIHAAKKLDREEKSLYILRAKAIDRKTGRQVEPESEFIIKIHDINDNEPKFTKDLYTASVPEMSGVGTSVIQVTATDADDANYGNSAKVVYSILQGQPYFSVDPESGIIKTALPDMSRENREQYQVVIQAKDMGGQMGGLSGTTTVNITLTDVNNNPPRFPQSTYQFNSPESVPLGTHLGRIKANDPDVGENAEMEYSIAEGDGADMFDVITDKDTQEGIITVKQNLDFENQMLYTLRVDASNTHPDPRFLHLGPFKDTAVVKISVEDIDEPPVFTKVSYLIEVDEDVKEGSIIGQVTAYDPDARNNLIKYSVDRHTDMDRIFGIHSENGSIFTLKALDRESSPWHNITVTATEINNPKQSSHIPVFIRILDINDHAPEFAMYYETFVCENAKPGQLIQTVSVMDKDDPPRGHKFFFEPVPEFTLNPNFTIVDNKDNTAGIMTRKDGYSRNKMSTYLLPILIFDNDYPIQSSTGTLTIRVCACDNQGNMQSCTAEALILSAGLSTGA

  • Molecular Weight

    Approximately 70-90 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, 6% trehalose, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) Volume (to add to vial)
=
Mass (in vial) Mass (in vial)
÷
Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

Concentration (start) Concentration (start)
×
Volume (start) Volume (start)
=
Concentration (final) Concentration (final)
×
Volume (final) Volume (final)
The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 90%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. When Recombinant Human Cadherin-9 is immobilized at 1 μg/mL can bind Biotinylated Human Cadherin-6. The ED50 for this effect is 5.36 μg/mL.

  • Cadherin-9/CDH9 Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for Cadherin-9/CDH9 Protein, Human (HEK293, His)
  • Experimental Validation Results for Cadherin-9/CDH9 Protein, Human (HEK293, His)
  • Cadherin-9/CDH9 Protein, Human (HEK293, His) Product Image