1. Recombinant Proteins
  2. Enzymes & Regulators
  3. Carboxypeptidase
  4. Carboxypeptidase B
  5. Carboxypeptidase B1
  6. Carboxypeptidase B1/CPB1 Protein, Human (HEK293, His)

Carboxypeptidase B1/CPB1 Protein, Human (HEK293, His)

Cat. No.: HY-P7734
COA Handling Instructions

Carboxypeptidase B1/CPB1 Protein, Human (HEK293, His) is an approximately 47.0 kDa carboxypeptidase B1 protein with a C-terminal His-flag.

For research use only. We do not sell to patients.

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50 μg $380 In-stock
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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

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  • Documentation

  • References

Description

Carboxypeptidase B1/CPB1 Protein, Human (HEK293, His) is an approximately 47.0 kDa carboxypeptidase B1 protein with a C-terminal His-flag.

Background

Carboxypeptidase B1 is encoded by the CPB1 gene. The deduced amino acid sequence of human CPB1 consists of a signal peptide (residues 1 to 15), a pro region (residue 16 to 110), and a mature chain (residues 111 to 417)[1].
Carboxypeptidase B (CPB) is a protease that specifically hydrolyzes C-terminal alkaline amino acid of a peptide[1]. As a result, it plays an important role in biological analysis. Carboxypeptidase B has a critical role in downregulating inflammatory responses in rheumatoid arthritis[2].

Biological Activity

Measured by its ability to cleave a colorimetric peptide substrate, Hippuryl-Arg. The specific activity is 12990.93 pmol/min/µg.

Species

Human

Source

HEK293

Tag

C-6*His

Accession

P15086 (H16-Y417)

Gene ID
Molecular Construction
N-term
CPB1 (H16-Y417)
Accession # P15086
6*His
C-term
Synonyms
rHuCarboxypeptidase B1, His; Carboxypeptidase B; Pancreas-Specific Protein; PASP; CPB1; CPB; PCPB
AA Sequence

HHGGEHFEGEKVFRVNVEDENHINIIRELASTTQIDFWKPDSVTQIKPHSTVDFRVKAEDTVTVENVLKQNELQYKVLISNLRNVVEAQFDSRVRATGHSYEKYNKWETIEAWTQQVATENPALISRSVIGTTFEGRAIYLLKVGKAGQNKPAIFMDCGFHAREWISPAFCQWFVREAVRTYGREIQVTELLDKLDFYVLPVLNIDGYIYTWTKSRFWRKTRSTHTGSSCIGTDPNRNFDAGWCEIGASRNPCDETYCGPAAESEKETKALADFIRNKLSSIKAYLTIHSYSQMMIYPYSYAYKLGENNAELNALAKATVKELASLHGTKYTYGPGATTIYPAAGGSDDWAYDQGIRYSFTFELRDTGRYGFLLPESQIRATCEETFLAIKYVASYVLEHLYHHHHHH

Molecular Weight

Approximately 47.0 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE. 

Appearance

Solution.

Formulation

Supplied as a 0.2 μm filter solution of 20 mM MES, 150 mM NaCl, pH 5.5.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation
References

Carboxypeptidase B1/CPB1 Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Carboxypeptidase B1/CPB1 Protein, Human (HEK293, His)
Cat. No.:
HY-P7734
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