Carboxypeptidase B1/CPB1 Protein, Human (HEK293, His)
Based on 1 Customer Validation
Carboxypeptidase B1/CPB1 Protein, Human (HEK293, His) is an approximately 47.0 kDa carboxypeptidase B1 protein with a C-terminal His-flag.
- Species: Human
- Source: HEK293
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- References
- Help & FAQs
Biological Activity
Description
Carboxypeptidase B1/CPB1 Protein, Human (HEK293, His) is an approximately 47.0 kDa carboxypeptidase B1 protein with a C-terminal His-flag.
Background
Carboxypeptidase B1 is encoded by the CPB1 gene. The deduced amino acid sequence of human CPB1 consists of a signal peptide (residues 1 to 15), a pro region (residue 16 to 110), and a mature chain (residues 111 to 417)[1].Carboxypeptidase B (CPB) is a protease that specifically hydrolyzes C-terminal alkaline amino acid of a peptide[1]. As a result, it plays an important role in biological analysis. Carboxypeptidase B has a critical role in downregulating inflammatory responses in rheumatoid arthritis[2].
Verified Bioactivity
Measured by its ability to cleave a colorimetric peptide substrate, Hippuryl-Arg. The specific activity is >10000 pmol/min/µg. (Activation description: The proenzyme needs to be activated by Trypsin for an activated form.)
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
Technical Parameters
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Species Human
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Source HEK293
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Tag C-6*His
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Accession
P15086/NP_001862.2 (H16-Y417)
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Molecular Construction
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N-term
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CPB1 (H16-Y417)
Accession # P15086/NP_001862.2 -
6*His
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C-term
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Protein Length
Full Length of Mature Protein
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Synonyms
CPB1; Pancreas-Specific Protein; Carboxypeptidase B1; Protaminase; Carboxypeptidase B; PASP; Pancreatic Carboxypeptidase B; PCPB; Carboxypeptidase B1 (Tissue); CPB; Tissue Carboxypeptidase B; Procarboxypeptidase B
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AA Sequence
HHGGEHFEGEKVFRVNVEDENHINIIRELASTTQIDFWKPDSVTQIKPHSTVDFRVKAEDTVTVENVLKQNELQYKVLISNLRNVVEAQFDSRVRATGHSYEKYNKWETIEAWTQQVATENPALISRSVIGTTFEGRAIYLLKVGKAGQNKPAIFMDCGFHAREWISPAFCQWFVREAVRTYGREIQVTELLDKLDFYVLPVLNIDGYIYTWTKSRFWRKTRSTHTGSSCIGTDPNRNFDAGWCEIGASRNPCDETYCGPAAESEKETKALADFIRNKLSSIKAYLTIHSYSQMMIYPYSYAYKLGENNAELNALAKATVKELASLHGTKYTYGPGATTIYPAAGGSDDWAYDQGIRYSFTFELRDTGRYGFLLPESQIRATCEETFLAIKYVASYVLEHLY
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Molecular Weight
Approximately 45-48 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Supplied as a 0.2 μm filter solution of 20 mM MES, 150 mM NaCl, pH 5.5.
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (264 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. K K Yamamoto, et al. Isolation of a cDNA encoding a human serum marker for acute pancreatitis. Identification of pancreas-specific protein as pancreatic procarboxypeptidase B. J Biol Chem. 1992 Feb 5;267(4):2575-81. [Content Brief]
[2]. J W Brodrick, et al. Human carboxypeptidase B. II. Purification of the enzyme from pancreatic tissue and comparison with the enzymes present in pancreatic secretion. Biochim Biophys Acta. 1976 Dec 8;452(2):468-81. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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