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Casein Kinase I gamma-2/CSNK1G2 Protein, Human (Active, sf9, His)

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Casein kinase I gamma-2 is a serine/threonine protein kinase that targets acidic proteins and phosphorylates a variety of substrates, such as COL4A3BP/CERT, MTA1 and SMAD3, especially destabilizing SMAD3 and inhibiting TGF- beta reaction. Hyperphosphorylation of COL4A3BP/CERT disrupts ER-to-Golgi transport and affects the synthesis of ceramide and sphingomyelin. Casein Kinase I gamma-2/CSNK1G2 Protein, Human (sf9, His) is the recombinant human-derived Casein Kinase I gamma-2 protein, expressed by Sf9 insect cells , with N-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: Sf9 insect cells
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Casein Kinase I gamma-2/CSNK1G2 Protein, Human (Active, sf9, His) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

Casein kinase I gamma-2 is a serine/threonine protein kinase that targets acidic proteins and phosphorylates a variety of substrates, such as COL4A3BP/CERT, MTA1 and SMAD3, especially destabilizing SMAD3 and inhibiting TGF- beta reaction. Hyperphosphorylation of COL4A3BP/CERT disrupts ER-to-Golgi transport and affects the synthesis of ceramide and sphingomyelin. Casein Kinase I gamma-2/CSNK1G2 Protein, Human (sf9, His) is the recombinant human-derived Casein Kinase I gamma-2 protein, expressed by Sf9 insect cells , with N-His labeled tag.

Background

Casein Kinase I gamma-2, a serine/threonine-protein kinase, exhibits a preference for acidic proteins, with caseins being prominent substrates. Demonstrating versatility, it phosphorylates a diverse array of proteins and actively participates in Wnt signaling. Noteworthy targets include COL4A3BP/CERT, MTA1, and SMAD3. Phosphorylation of SMAD3 serves to destabilize the protein, leading to ligand-dependent ubiquitination and subsequent proteasome degradation, thereby inhibiting TGF-beta responses. Hyperphosphorylation of the serine-repeat motif in COL4A3BP/CERT results in its inactivation, disrupting ER-to-Golgi transport and suppressing ceramide and sphingomyelin synthesis. Additionally, Casein Kinase I gamma-2 plays crucial roles in brain development, vesicular trafficking, neurotransmitter release from synaptic vesicles, and the regulation of fast synaptic transmission mediated by glutamate. Moreover, it contributes to the control of reactive oxygen species (ROS) levels, further emphasizing its multifaceted involvement in cellular processes.

Verified Bioactivity

The specific activity was determined to be 43 nmol/min/mg using casein as substrate.

Technical Parameters

  • Species Human
  • Source Sf9 insect cells
  • Tag N-His
  • Accession

    P78368 (M18-K415)

  • Gene ID
    1455  [NCBI]
  • Molecular Construction
    • N-term
    • His
    • CSNK1G2 (M18-K415)
      Accession # P78368-1
    • C-term
  • Protein Length

    Partial

  • Synonyms

    CSNK1G2; Non-Specific Serine/Threonine Protein Kinase; Casein Kinase 1 Gamma 2; Casein Kinase 1, Gamma 2; CK1g2; Casein Kinase I; Casein Kinase I Isoform Gamma-2; CK1G2; CKI-Gamma 2

  • AA Sequence

    MSKAGGGRSSHGIRSSGTSSGVLMVGPNFRVGKKIGCGNFGELRLGKNLYTNEYVAIKLEPIKSRAPQLHLEYRFYKQLSATEGVPQVYYFGPCGKYNAMVLELLGPSLEDLFDLCDRTFTLKTVLMIAIQLITRMEYVHTKSLIYRDVKPENFLVGRPGTKRQHAIHIIDFGLAKEYIDPETKKHIPYREHKSLTGTARYMSINTHLGKEQSRRDDLEALGHMFMYFLRGSLPWQGLKADTLKERYQKIGDTKRATPIEVLCENFPEEMATYLRYVRRLDFFEKPDYDYLRKLFTDLFDRSGFVFDYEYDWAGKPLPTPIGTVHTDLPSQPQLRDKTQPHSKNQALNSTNGELNADDPTAGHSNAPITAPAEVEVADETKCCCFFKRRKRKSLQRHK

  • Predicted Molecular Mass

    47.8 kDa

  • Molecular Weight

    Approximately 48 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution.

Formulation

Supplied as a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, 10% gly, 1 mM DTT, pH 8.0
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

Please use rapid thawing with running water to thaw the protein.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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  • Casein Kinase I gamma-2/CSNK1G2 Protein, Human (Active, sf9, His) Product Image