Casein Kinase I gamma-2 Protein, Human (sf9, His)

Casein kinase I gamma-2 is a serine/threonine protein kinase that targets acidic proteins and phosphorylates a variety of substrates, such as COL4A3BP/CERT, MTA1 and SMAD3, especially destabilizing SMAD3 and inhibiting TGF- beta reaction. Hyperphosphorylation of COL4A3BP/CERT disrupts ER-to-Golgi transport and affects the synthesis of ceramide and sphingomyelin. Casein Kinase I gamma-2 Protein, Human (sf9, His) is the recombinant human-derived Casein Kinase I gamma-2 protein, expressed by Sf9 insect cells , with N-His labeled tag. The total length of Casein Kinase I gamma-2 Protein, Human (sf9, His) is 398 a.a., with molecular weight of ~48 kDa.

Casein Kinase I gamma-2, a serine/threonine-protein kinase, exhibits a preference for acidic proteins, with caseins being prominent substrates. Demonstrating versatility, it phosphorylates a diverse array of proteins and actively participates in Wnt signaling. Noteworthy targets include COL4A3BP/CERT, MTA1, and SMAD3. Phosphorylation of SMAD3 serves to destabilize the protein, leading to ligand-dependent ubiquitination and subsequent proteasome degradation, thereby inhibiting TGF-beta responses. Hyperphosphorylation of the serine-repeat motif in COL4A3BP/CERT results in its inactivation, disrupting ER-to-Golgi transport and suppressing ceramide and sphingomyelin synthesis. Additionally, Casein Kinase I gamma-2 plays crucial roles in brain development, vesicular trafficking, neurotransmitter release from synaptic vesicles, and the regulation of fast synaptic transmission mediated by glutamate. Moreover, it contributes to the control of reactive oxygen species (ROS) levels, further emphasizing its multifaceted involvement in cellular processes.

Human

Sf9 insect cells

N-His

P78368 (M18-K415)

1455  [NCBI]

MSKAGGGRSSHGIRSSGTSSGVLMVGPNFRVGKKIGCGNFGELRLGKNLYTNEYVAIKLEPIKSRAPQLHLEYRFYKQLSATEGVPQVYYFGPCGKYNAMVLELLGPSLEDLFDLCDRTFTLKTVLMIAIQLITRMEYVHTKSLIYRDVKPENFLVGRPGTKRQHAIHIIDFGLAKEYIDPETKKHIPYREHKSLTGTARYMSINTHLGKEQSRRDDLEALGHMFMYFLRGSLPWQGLKADTLKERYQKIGDTKRATPIEVLCENFPEEMATYLRYVRRLDFFEKPDYDYLRKLFTDLFDRSGFVFDYEYDWAGKPLPTPIGTVHTDLPSQPQLRDKTQPHSKNQALNSTNGELNADDPTAGHSNAPITAPAEVEVADETKCCCFFKRRKRKSLQRHK

Approximately 48 kDa

Greater than 80% as determined by reducing SDS-PAGE

<1 EU/μg, determined by LAL method.