Casein Kinase I gamma-2/CSNK1G2 Protein, Human (Active, sf9, His)

Casein kinase I gamma-2 is a serine/threonine protein kinase that targets acidic proteins and phosphorylates a variety of substrates, such as COL4A3BP/CERT, MTA1 and SMAD3, especially destabilizing SMAD3 and inhibiting TGF- beta reaction. Hyperphosphorylation of COL4A3BP/CERT disrupts ER-to-Golgi transport and affects the synthesis of ceramide and sphingomyelin. Casein Kinase I gamma-2/CSNK1G2 Protein, Human (sf9, His) is the recombinant human-derived Casein Kinase I gamma-2 protein, expressed by Sf9 insect cells , with N-His labeled tag.

Casein Kinase I gamma-2, a serine/threonine-protein kinase, exhibits a preference for acidic proteins, with caseins being prominent substrates. Demonstrating versatility, it phosphorylates a diverse array of proteins and actively participates in Wnt signaling. Noteworthy targets include COL4A3BP/CERT, MTA1, and SMAD3. Phosphorylation of SMAD3 serves to destabilize the protein, leading to ligand-dependent ubiquitination and subsequent proteasome degradation, thereby inhibiting TGF-beta responses. Hyperphosphorylation of the serine-repeat motif in COL4A3BP/CERT results in its inactivation, disrupting ER-to-Golgi transport and suppressing ceramide and sphingomyelin synthesis. Additionally, Casein Kinase I gamma-2 plays crucial roles in brain development, vesicular trafficking, neurotransmitter release from synaptic vesicles, and the regulation of fast synaptic transmission mediated by glutamate. Moreover, it contributes to the control of reactive oxygen species (ROS) levels, further emphasizing its multifaceted involvement in cellular processes.

The specific activity was determined to be 43 nmol/min/mg using casein as substrate.

Human

Sf9 insect cells

N-His

P78368 (M18-K415)

1455  [NCBI]

Partial

MSKAGGGRSSHGIRSSGTSSGVLMVGPNFRVGKKIGCGNFGELRLGKNLYTNEYVAIKLEPIKSRAPQLHLEYRFYKQLSATEGVPQVYYFGPCGKYNAMVLELLGPSLEDLFDLCDRTFTLKTVLMIAIQLITRMEYVHTKSLIYRDVKPENFLVGRPGTKRQHAIHIIDFGLAKEYIDPETKKHIPYREHKSLTGTARYMSINTHLGKEQSRRDDLEALGHMFMYFLRGSLPWQGLKADTLKERYQKIGDTKRATPIEVLCENFPEEMATYLRYVRRLDFFEKPDYDYLRKLFTDLFDRSGFVFDYEYDWAGKPLPTPIGTVHTDLPSQPQLRDKTQPHSKNQALNSTNGELNADDPTAGHSNAPITAPAEVEVADETKCCCFFKRRKRKSLQRHK

Approximately 48 kDa,based on SDS-PAGE under reducing conditions.

≥ 90%, as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, 10% gly, 1 mM DTT, pH 8.0
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.