CBFB Protein, Human (His)

CBFB, forming the heterodimeric complex core-binding factor (CBF) with RUNX family proteins, enhances the DNA-binding capacity of RUNX members like RUNX1, RUNX2, and RUNX3. The heterodimers bind to specific sites in enhancers and promoters, modulating transcription of target genes. CBFB complexes repress ZBTB7B during CD8+ T cell development, acting as a transcriptional silencer and facilitating cytotoxic T cell differentiation. Interactions occur with COPRS and PRMT5 in complexes with RUNX1. CBFB Protein, Human (His) is the recombinant human-derived CBFB protein, expressed by E. coli , with N-6*His labeled tag. The total length of CBFB Protein, Human (His) is 181 a.a., with molecular weight of ~25 kDa.

CBFB, a core-binding factor, forms the heterodimeric complex, CBF, with RUNX family proteins, including RUNX1, RUNX2, and RUNX3. RUNX members exert transcriptional modulation on their target genes by recognizing the core consensus binding sequence 5'-TGTGGT-3', or exceptionally, 5'-TGCGGT-3', within regulatory regions through their runt domain. CBFB serves as a non-DNA-binding regulatory subunit that enhances the sequence-specific DNA-binding capacity of RUNX allosterically. These heterodimers bind to the core sites of various enhancers and promoters, such as murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3, and GM-CSF promoters. CBF complexes play a role in the repression of the ZBTB7B transcription factor during cytotoxic (CD8+) T cell development, acting as a transcriptional silencer by binding to the RUNX-binding sequence within the ZBTB7B locus, thereby facilitating cytotoxic T cell differentiation. The interaction involves heterodimerization with RUNX1, RUNX2, and RUNX3, and CBFB also interacts with COPRS and forms a complex with PRMT5 and RUNX1.

Data is not available.

Human

E. coli

N-6*His

Q13951-1 (P2-P182)

865  [NCBI]

PRVVPDQRSKFENEEFFRKLSRECEIKYTGFRDRPHEERQARFQNACRDGRSEIAFVATGTNLSLQFFPASWQGEQRQTPSREYVDLEREAGKVYLKAPMILNGVCVIWKGWIDLQRLDGMGCLEFDEERAQQEDALAQQAFEEARRRTREFEDRDRSHREEMEVRVSQLLAVTGKKTTRP

Approximately 25 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of sterile 50 mM Tris, 300 mM NaCl, 5% trehalose, 5% mannitol and 0.01% Tween 80, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.