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CD131 Protein, Human (HEK293, His)

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Based on 1 Customer Validation

The CD131 protein is a cell surface receptor that plays a critical role in the immune response and controls the generation and differentiation of hematopoietic progenitor cells. CD131 Protein, Human (HEK293, His) is the recombinant human-derived CD131 protein, expressed by HEK293 , with C-10*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Experimental Validation Results for CD131 Protein, Human (HEK293, His)
  • Experimental Validation Results for CD131 Protein, Human (HEK293, His)
  • Experimental Validation Results for CD131 Protein, Human (HEK293, His)
  • CD131 Protein, Human (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

The CD131 protein is a cell surface receptor that plays a critical role in the immune response and controls the generation and differentiation of hematopoietic progenitor cells. CD131 Protein, Human (HEK293, His) is the recombinant human-derived CD131 protein, expressed by HEK293 , with C-10*His labeled tag.

Background

CD131, a cell surface receptor, plays a pivotal role in immune response by controlling the production and differentiation of hematopoietic progenitor cells into lineage-restricted cells. Through the formation of a heterodimeric receptor with various partners such as IL3RA, IL5RA, or CSF2RA, CD131 engages in multiple signaling pathways, including interleukin-3, interleukin-5, and granulocyte-macrophage colony-stimulating factor/CSF2 pathways. In unstimulated conditions, CD131 constitutively interacts with JAK1, and ligand binding leads to JAK1 stimulation, triggering the activation of the JAK-STAT pathway. CD131 forms a heterodimer composed of an alpha and a beta subunit, with the beta subunit being common to the IL3, IL5, and GM-CSF receptors. The GM-CSF receptor complex, involved in signaling, is a dodecamer consisting of two head-to-head hexamers of two alpha, two beta, and two ligand subunits. CD131 further interacts with TMEM102, FCER1G, LYN, and JAK1, contributing to its intricate role in cellular responses.

Verified Bioactivity

1.Measured by its binding ability in a functional ELISA. Recombinant Human CD131 binds Recombinant Human GM-CSF (5 μg/mL, 100 μL/well) in the presence of Recombinant Human GM-CSF R alpha. The ED50 for this effect is <120 ng/mL.
2. Loaded Trabikibart (HY-P990094) on ProA biosensor, can bind CD131 Protein, Human with an affinity constant of 9.994E-10 M as determined in BLI assay.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-10*His
  • Accession

    P32927-1 (W17-W443)

  • Gene ID
    1439  [NCBI]
  • Molecular Construction
    • N-term
    • CD131 (W17-W443)
      Accession # P32927
    • 10*His
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    CSF2RB; Beta Common Cytokine Receptor; Prev. IL3RB; CDw131; IL5RB; Interleukin 3 Receptor/Granulocyte-Macrophage Colony Stimulating Factor 3 Receptor, Beta (High Affinity); GM-CSF/IL-3/IL-5 Receptor Common Beta Subunit; Colony-Stimulating Factor-2 Recepto

  • AA Sequence

    WERSLAGAEETIPLQTLRCYNDYTSHITCRWADTQDAQRLVNVTLIRRVNEDLLEPVSCDLSDDMPWSACPHPRCVPRRCVIPCQSFVVTDVDYFSFQPDRPLGTRLTVTLTQHVQPPEPRDLQISTDQDHFLLTWSVALGSPQSHWLSPGDLEFEVVYKRLQDSWEDAAILLSNTSQATLGPEHLMPSSTYVARVRTRLAPGSRLSGRPSKWSPEVCWDSQPGDEAQPQNLECFFDGAAVLSCSWEVRKEVASSVSFGLFYKPSPDAGEEECSPVLREGLGSLHTRHHCQIPVPDPATHGQYIVSVQPRRAEKHIKSSVNIQMAPPSLNVTKDGDSYSLRWETMKMRYEHIDHTFEIQYRKDTATWKDSKTETLQNAHSMALPALEPSTRYWARVRVRTSRTGYNGIWSEWSEARSWDTESVLPMW

  • Predicted Molecular Mass

    50 kDa

  • Molecular Weight

    Approximately 50-55 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. Recombinant Human CD131 binds Recombinant Human GM-CSF (5 μg/mL, 100 μL/well) in the presence of Recombinant Human GM-CSF R alpha. The ED50 for this effect is 96.35 ng/mL.

  • 1/16

    MedChemExpress Validation
     Loaded Trabikibart (HY-P990094) on ProA biosensor, can bind CD131 Protein, Human with an affinity constant of 9.994E-10 M as determined in BLI assay.

  • CD131 Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for CD131 Protein, Human (HEK293, His)
  • Experimental Validation Results for CD131 Protein, Human (HEK293, His)
  • Experimental Validation Results for CD131 Protein, Human (HEK293, His)
  • CD131 Protein, Human (HEK293, His) Product Image