CD146/MCAM Protein, Human (Biotinylated, HEK293, His)
The CD146/MCAM protein is critical for maintaining endothelial monolayer integrity and cell adhesion in vascular tissue. Its expression promotes the interaction of melanoma cells with the vasculature and may contribute to tumor spread through the bloodstream. CD146/MCAM Protein, Human (Biotinylated, HEK293, His) is the recombinant human-derived CD146/MCAM protein, expressed by HEK293 , with C-His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The CD146/MCAM protein is critical for maintaining endothelial monolayer integrity and cell adhesion in vascular tissue. Its expression promotes the interaction of melanoma cells with the vasculature and may contribute to tumor spread through the bloodstream. CD146/MCAM Protein, Human (Biotinylated, HEK293, His) is the recombinant human-derived CD146/MCAM protein, expressed by HEK293 , with C-His labeled tag.
Background
The CD146/MCAM protein plays a crucial role in cell adhesion and maintaining the integrity of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression enables melanoma cells to interact with the cellular components of the vascular system, potentially promoting the spread of tumors through the bloodstream. During embryonic development, CD146/MCAM may function as an adhesion molecule in neural crest cells. It acts as a surface receptor, initiating the tyrosine phosphorylation of FYN and PTK2/FAK1, and leading to a transient rise in intracellular calcium levels.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-His
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Accession
P43121-1/NP_006491.2 (V24-G559)
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Molecular Construction
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N-term
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MCAM (V24-G559)
Accession # P43121-1/NP_006491.2 -
His
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C-term
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Protein Length
Extracellular Domain
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Conjugation
Biotin
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Synonyms
MCAM; Melanoma-Associated Antigen MUC18; Melanoma Cell Adhesion Molecule; Cell Surface Glycoprotein MUC18; MUC18; Cell Surface Glycoprotein P1H12; MelCAM; Melanoma-Associated Antigen A32; METCAM; Melanoma Adhesion Molecule; HEMCAM; Gicerin; CD146; CD146 A
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AA Sequence
VPGEAEQPAPELVEVEVGSTALLKCGLSQSQGNLSHVDWFSVHKEKRTLIFRVRQGQGQSEPGEYEQRLSLQDRGATLALTQVTPQDERIFLCQGKRPRSQEYRIQLRVYKAPEEPNIQVNPLGIPVNSKEPEEVATCVGRNGYPIPQVIWYKNGRPLKEEKNRVHIQSSQTVESSGLYTLQSILKAQLVKEDKDAQFYCELNYRLPSGNHMKESREVTVPVFYPTEKVWLEVEPVGMLKEGDRVEIRCLADGNPPPHFSISKQNPSTREAEEETTNDNGVLVLEPARKEHSGRYECQGLDLDTMISLLSEPQELLVNYVSDVRVSPAAPERQEGSSLTLTCEAESSQDLEFQWLREETGQVLERGPVLQLHDLKREAGGGYRCVASVPSIPGLNRTQLVNVAIFGPPWMAFKERKVWVKENMVLNLSCEASGHPRPTISWNVNGTASEQDQDPQRVLSTLNVLVTPELLETGVECTASNDLGKNTSILFLELVNLTTLTPDSNTTTGLSTSTASPHTRANSTSTERKLPEPESRG
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Predicted Molecular Mass
61.3 kDa
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Molecular Weight
Approximately 89 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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