CD24 Protein, Rat (HEK293, Fc)

The CD24 protein plays a critical role in cellular differentiation in various cell types, possibly triggered by binding of lectin-like ligands to the CD24 carbohydrate. This signal is transduced through second messengers released from GPI anchors. CD24 Protein, Rat (HEK293, Fc) is the recombinant rat-derived CD24 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of CD24 Protein, Rat (HEK293, Fc) is 29 a.a., with molecular weight of 45-57 kDa.

The CD24 protein appears to play a pivotal role in the cell differentiation of various cell types, with signaling potentially initiated by the binding of a lectin-like ligand to CD24 carbohydrates. This signal is transduced through the release of second messengers derived from the GPI-anchor. Additionally, CD24 is involved in modulating B-cell activation responses and, in association with SIGLEC10, may contribute to the selective suppression of the immune response to danger-associated molecular patterns (DAMPs) like HMGB1, HSP70, and HSP90. These findings suggest a multifaceted role for CD24, encompassing its influence on cell differentiation, immune response regulation, and potential contributions to the control of autoimmunity. Further exploration into the specific mechanisms underlying these functions could provide valuable insights into the diverse roles played by CD24 in cellular processes.

When Recombinant Rat CD24 Protein is immobilized at 2 µg/mL (100 µL/well) can bind Anti-CD24 antibody, The ED₅₀ for this effect is 2.235 µg/mL.

Rat

HEK293

C-hFc

Q07490 (N27-C55)

25145  [NCBI]

NQTSVAPFSGNQSISAAPNPTNATTRSGC

Approximately 45-57 kDa due to the glycosylation.

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.