1. Recombinant Proteins
  2. Cytokines and Growth Factors CD Antigens
  3. TNF Superfamily T Cell CD Proteins Macrophage CD Proteins
  4. TNF Superfamily Ligands CD153/CD30L
  5. CD30 Ligand
  6. CD30 Ligand/TNFSF8 Protein, Human (HEK293, N-His)

CD30 Ligand/TNFSF8 Protein, Human (HEK293, N-His)

Cat. No.: HY-P700434
Handling Instructions

CD30 ligand/TNFSF8 protein is a cytokine that specifically binds to TNFRSF8/CD30 and acts as a potent inducer of T cell proliferation. As a homotrimer, this ligand plays a crucial role in regulating T cell activation and growth, contributing to the dynamic coordination of immune responses. CD30 Ligand/TNFSF8 Protein, Human (HEK293, N-His) is the recombinant human-derived CD30 Ligand/TNFSF8 protein, expressed by HEK293 , with N-6*His labeled tag. The total length of CD30 Ligand/TNFSF8 Protein, Human (HEK293, N-His) is 172 a.a., with molecular weight of 21.8 kDa.

For research use only. We do not sell to patients.

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Description

CD30 ligand/TNFSF8 protein is a cytokine that specifically binds to TNFRSF8/CD30 and acts as a potent inducer of T cell proliferation. As a homotrimer, this ligand plays a crucial role in regulating T cell activation and growth, contributing to the dynamic coordination of immune responses. CD30 Ligand/TNFSF8 Protein, Human (HEK293, N-His) is the recombinant human-derived CD30 Ligand/TNFSF8 protein, expressed by HEK293 , with N-6*His labeled tag. The total length of CD30 Ligand/TNFSF8 Protein, Human (HEK293, N-His) is 172 a.a., with molecular weight of 21.8 kDa.

Background

CD30 Ligand/TNFSF8 protein, a cytokine, specifically binds to TNFRSF8/CD30, acting as a potent inducer of T-cell proliferation. Operating as a homotrimer, this ligand plays a crucial role in regulating the activation and growth of T-cells, contributing to the dynamic orchestration of immune responses. The interaction between CD30 Ligand and its receptor, TNFRSF8/CD30, highlights its significance as a molecular trigger for the robust expansion of T-cell populations.

Species

Human

Source

HEK293

Tag

N-6*His

Accession

P32971 (Q63-D234)

Gene ID

944  [NCBI]

Molecular Construction
N-term
6*His
CD30L (Q63-D234)
Accession # P32971
C-term
Synonyms
Tumor necrosis factor ligand superfamily member 8; CD153; CD30L; CD30LG
AA Sequence

QRTDSIPNSPDNVPLKGGNCSEDLLCILKRAPFKKSWAYLQVAKHLNKTKLSWNKDGILHGVRYQDGNLVIQFPGLYFIICQLQFLVQCPNNSVDLKLELLINKHIKKQALVTVCESGMQTKHVYQNLSQFLLDYLQVNTTISVNVDTFQYIDTSTFPLENVLSIFLYSNSD

Molecular Weight

21.8 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
CD30 Ligand/TNFSF8 Protein, Human (HEK293, N-His)
Cat. No.:
HY-P700434
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