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CDK1-CCNE1 Heterodimer Protein, Human (Inactive, sf9, His-GST)

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Based on 1 Customer Validation

The FGF-9 protein undergoes autoproteolysis and cholesterol attachment in the endoplasmic reticulum. It acts as a morphogen during development, inducing ventral cell fate, participating in limb bud patterning, and aiding in axon guidance. FGF-9 binds to the PTCH1 receptor and activates target gene transcription when in association with SMO, while PTCH1 represses SMO signaling in the absence of FGF-9. CDK1-CCNE1 Heterodimer Protein, Human (sf9, His-GST) is a recombinant protein dimer complex containing human-derived CDK1-CCNE1 Heterodimer protein, expressed by Sf9 insect cells , with N-10*His, N-GST labeled tag. CDK1-CCNE1 Heterodimer Protein, Human (sf9, His-GST), has molecular weight of ~109.7 (60.4+49.3) kDa.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: Sf9 insect cells
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Experimental Validation Results for CDK1-CCNE1 Heterodimer Protein, Human (Inactive, sf9, His-GST)
  • CDK1-CCNE1 Heterodimer Protein, Human (Inactive, sf9, His-GST) Product Image
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Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

The FGF-9 protein undergoes autoproteolysis and cholesterol attachment in the endoplasmic reticulum. It acts as a morphogen during development, inducing ventral cell fate, participating in limb bud patterning, and aiding in axon guidance. FGF-9 binds to the PTCH1 receptor and activates target gene transcription when in association with SMO, while PTCH1 represses SMO signaling in the absence of FGF-9. CDK1-CCNE1 Heterodimer Protein, Human (sf9, His-GST) is a recombinant protein dimer complex containing human-derived CDK1-CCNE1 Heterodimer protein, expressed by Sf9 insect cells , with N-10*His, N-GST labeled tag. CDK1-CCNE1 Heterodimer Protein, Human (sf9, His-GST), has molecular weight of ~109.7 (60.4+49.3) kDa.

Background

The CDK1 Protein, a member of the Ser/Thr protein kinase family, serves as the catalytic subunit in the highly conserved M-phase promoting factor (MPF) complex crucial for G2/M phase transitions in the eukaryotic cell cycle. Mitotic cyclins form stable associations with this protein, acting as regulatory subunits. The kinase activity is finely regulated through the accumulation and degradation of cyclins across the cell cycle. Phosphorylation and dephosphorylation events further contribute to the intricate regulatory mechanisms governing cell cycle control. Notably, the CDK1 Protein demonstrates broad expression, particularly prevalent in the lymph nodes (RPKM 12.8), bone marrow (RPKM 12.8), and 18 other tissues, suggesting its involvement in diverse physiological processes across multiple organ systems. Various alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene.

Verified Bioactivity

1.The product demonstrated no detectable kinase activity in an ATP/ADP-based kinase assay.
2.Measured by its binding ability in a functional ELISA.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 90%, as determined by reducing SDS-PAGE.

Technical Parameters

  • Species Human
  • Source Sf9 insect cells
  • Tag N-10*His;N-GST
  • Accession

    NP_001777.1 (M1-M297)&NP_001229.1 (M1-A410)

  • Gene ID
    983  [NCBI]&898  [NCBI]
  • Synonyms

    CDK1; Cell Division Protein Kinase 1; Prev. CDC2; P34 Protein Kinase; CDC28A; P34CDC2; Cell Division Cycle 2, G1 To S And G2 To M; Cell Cycle Controller CDC2; Cell Division Control Protein 2 Homolog; CDKN1; Cyclin Dependent Kinase 1; Cyclin-Dependent Kinase 1; CCNE1; Cyclin E Variant Ex7del; Prev. CCNE; Cyclin Et; G1/S-Specific Cyclin-E1; PCCNE1; Cyclin E Variant Ex5del; Cyclin E1; Cyclin Es

  • AA Sequence

    MEDYTKIEKIGEGTYGVVYKGRHKTTGQVVAMKKIRLESEEEGVPSTAIREISLLKELRHPNIVSLQDVLMQDSRLYLIFEFLSMDLKKYLDSIPPGQYMDSSLVKSYLYQILQGIVFCHSRRVLHRDLKPQNLLIDDKGTIKLADFGLARAFGIPIRVYTHEVVTLWYRSPEVLLGSARYSTPVDIWSIGTIFAELATKKPLFHGDSEIDQLFRIFRALGTPNNEVWPEVESLQDYKNTFPKWKPGSLASHVKNLDENGLDLLSKMLIYDPAKRISGKMALNHPYFNDLDNQIKKM&MPRERRERDAKERDTMKEDGGAEFSARSRKRKANVTVFLQDPDEEMAKIDRTARDQCGSQPWDNNAVCADPCSLIPTPDKEDDDRVYPNSTCKPRIIAPSRGSPLPVLSWANREEVWKIMLNKEKTYLRDQHFLEQHPLLQPKMRAILLDWLMEVCEVYKLHRETFYLAQDFFDRYMATQENVVKTLLQLIGISSLFIAAKLEEIYPPKLHQFAYVTDGACSGDEILTMELMIMKALKWRLSPLTIVSWLNVYMQVAYLNDLHEVLLPQYPQQIFIQIAELLDLCVLDVDCLEFPYGILAASALYHFSSSELMQKVSGYQWCDIENCVKWMVPFAMVIRETGSSKLKHFRGVADEDAHNIQTHRDSLDLLDKARAKKAMLSEQNRASPLPSGLLTPPQSGKKQSSGPEMA

  • Predicted Molecular Mass

    60.4 kDa (CCNE1) & 49.3 kDa (CDK1)

  • Molecular Weight

    Approximately 53 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution.

Formulation

Supplied as a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, 2 mM GSH, 10% Glycerol, pH 8.0, 5% trehalose, 5% mannitol and 0.01% Tween 80.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

Please use rapid thawing with running water to thaw the protein.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 90%, as determined by reducing SDS-PAGE.

  • CDK1-CCNE1 Heterodimer Protein, Human (Inactive, sf9, His-GST) Product Image
    1/16
  • Experimental Validation Results for CDK1-CCNE1 Heterodimer Protein, Human (Inactive, sf9, His-GST)
  • CDK1-CCNE1 Heterodimer Protein, Human (Inactive, sf9, His-GST) Product Image