CDK2-CCNE1 Protein, Human (Active, sf9, GST, His, Flag)

CDK2 is an important serine/threonine protein kinase that plays a critical regulatory role in the cell cycle, especially during meiosis. It phosphorylates various substrates (CTNNB1, USP37, p53/TP53, NPM1, CDK7, RB1, BRCA2, MYC, NPAT, EZH2) and coordinates centrosome duplication and initiation of DNA synthesis at the G1-S transition. CDK2-CCNE1 Protein, Human (sf9, GST, His, Flag) is the recombinant human-derived CDK2-CCNE1, expressed by Sf9 insect cells, with N-His, N-GST, N-Flag labeled tag.

CDK2, a serine/threonine-protein kinase, intricately regulates the cell cycle, playing an indispensable role in meiosis while being dispensable for mitosis. Operating at the nexus of cellular processes, CDK2 phosphorylates a diverse array of substrates, including CTNNB1, USP37, p53/TP53, NPM1, CDK7, RB1, BRCA2, MYC, NPAT, and EZH2. It orchestrates critical events such as centrosome duplication and DNA synthesis initiation at the G1-S transition, modulating G2 progression and finely tuning the balance between cellular proliferation, death, and DNA repair in human embryonic stem cells. Activated sequentially by cyclin E and cyclin A2, CDK2 regulates the transition from S phase to mitosis. Notably, its phosphorylation of EZH2 is implicated in maintaining H3K27me3 and epigenetic gene silencing. CDK2's involvement extends to DNA damage responses, where it regulates the G1-S phase checkpoint, phosphorylates BRCA2, and modulates homologous recombination-dependent repair. Furthermore, CDK2 participates in diverse signaling pathways, including vitamin D-mediated growth inhibition, NO-mediated signaling, and the phosphorylation of substrates such as FOXP3 and ERCC6, highlighting its multifaceted role in cellular physiology.

The activity was measured by off-chip mobility shift assay(MSA). The enzyme was incubated with fluorecence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by MSA device. The Km of CDK2-CCNE1 for its substrate is 100 μM.

Human

Sf9 insect cells

N-His;N-GST;N-Flag

P24941-1 (M1-L298)&P24864-1 (M1-E410)

1017&898

60.5 kDa & 51.1 kDa (~59 KDa/50 KDa)

≥ 90%, as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, pH 7.5, 200 mM NaCl, 5% glycerol.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.