CLEC2B Protein, Human (HEK293, Fc)
Based on 1 Customer Validation
CLEC2B Protein, Human (HEK293, Fc) is the recombinant human-derived CLEC2B protein, expressed by HEK293, with N-hFc labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
CLEC2B Protein, Human (HEK293, Fc) is the recombinant human-derived CLEC2B protein, expressed by HEK293, with N-hFc labeled tag.
Background
CLEC2B is a membrane-bound protein expressed on myeloid cells that acts as a ligand to stimulate the activating receptor NKp80/KLRF1, expressed on the surface of natural killer (NK) cells. This interaction stimulates NK-cell cytotoxicity and cytokine production, leading to the cytolysis of malignant CLEC2B-expressing myeloid cells.
Verified Bioactivity
Measured by its binding ability in a functional ELISA. When Recombinant Human AICL/CLEC-2B is coated at 2 µg/mL can bind Biotinylated Human Galectin-1 (HY-P70273). The ED50 for this effect is 3.87 µg/mL.
MCE Validation Data
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Bioactivity - ELISA
Bioactivity - ELISA
Technical Parameters
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Species Human
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Source HEK293
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Tag N-hFc
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Accession
Q92478 (K26-H149)
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Gene ID9976 [NCBI]
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Molecular Construction
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N-term
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hFc
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CLEC2B (K26-H149)
Accession # Q92478 -
C-term
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Protein Length
Extracellular Domain
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Synonyms
CLEC2B; IFN-Alpha-2b-Inducing-Related Protein 1; Prev. CLECSF2; Activation-Induced C-Type Lectin; AICL; IFNRG1; HP10085; C-Type Lectin Domain Family 2, Member B; C-Type (Calcium Dependent, Carbohydrate-Recognition Domain) Lectin, Superfamily Member 2 (Act
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AA Sequence
KLTRDSQSLCPYDWIGFQNKCYYFSKEEGDWNSSKYNCSTQHADLTIIDNIEEMNFLRRYKCSSDHWIGLKMAKNRTGQWVDGATFTKSFGMRGSEGCAYLSDDGAATARCYTERKWICRKRIH
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Predicted Molecular Mass
40 kDa
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Molecular Weight
Approximately 50-65 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 90%, as determined by reducing SDS-PAGE or Bis-Tris PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (235 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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