CNKSR3 Protein, Human (sf9, GST)
CNKSR3 protein regulates transepithelial sodium transport by controlling aldosterone-induced ENaC-mediated sodium transport. As a scaffolding protein, CNKSR3 coordinates ENaC regulatory complex (ERC) assembly and directly interacts with ENaC subunits SCNN1A and SCNN1B. CNKSR3 Protein, Human (sf9, GST) is the recombinant human-derived CNKSR3 protein, expressed by Sf9 insect cells , with N-GST labeled tag.
- Species: Human
- Source: Sf9 insect cells
-
Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Technical Parameters
-
Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
CNKSR3 protein regulates transepithelial sodium transport by controlling aldosterone-induced ENaC-mediated sodium transport. As a scaffolding protein, CNKSR3 coordinates ENaC regulatory complex (ERC) assembly and directly interacts with ENaC subunits SCNN1A and SCNN1B. CNKSR3 Protein, Human (sf9, GST) is the recombinant human-derived CNKSR3 protein, expressed by Sf9 insect cells , with N-GST labeled tag.
Background
The CNKSR3 Protein plays a pivotal role in the modulation of transepithelial sodium transport by regulating aldosterone-induced and epithelial sodium channel (ENaC)-mediated sodium transport through precise control of ENaC cell surface expression. Functioning as a scaffold protein, CNKSR3 orchestrates the assembly of an ENaC-regulatory complex (ERC). Its direct interaction with the C-terminal tails of ENaC subunits, SCNN1A α and SCNN1B β, underscores its involvement in coordinating the formation of the regulatory complex. Moreover, CNKSR3 interacts with key players in the ENaC regulatory network, including NEDD4L, RAF1, and SGK1, highlighting its role as a central mediator in the intricate regulation of sodium transport across epithelial cells. The multifaceted interactions of CNKSR3 emphasize its significance in the fine-tuning of sodium homeostasis and underline its position as a crucial player in the complex machinery governing ion transport processes.
Technical Parameters
-
Species Human
-
Source Sf9 insect cells
-
Tag N-GST
-
Accession
Q6P9H4-1 (M1-V352)
-
Molecular Construction
-
N-term
-
GST
-
CNKSR3 (M1-V352)
Accession # Q6P9H4-1 -
C-term
-
-
Protein Length
Partial
-
Synonyms
CNKSR3; FLJ31349; Prev. MAGI1; Membrane Associated Guanylate Kinase, WW And PDZ Domain Containing 1; Connector Enhancer Of Kinase Suppressor Of Ras 3; Membrane Associated Guanylate Kinase Interacting Protein-Like 1; Connector Enhancer Of KSR 3; Membrane-A
-
AA Sequence
MEPVTKWSPKQVVDWTRGLDDCLQQYVHKFEREKINGEQLLQISHQDLEELGVTRIGHQELVLEAVDLLCALNYGLETDNMKNLVLKLRASSHNLQNYISSRRKSPAYDGNTSRKAPNEFLTSVVELIGAAKALLAWLDRAPFTGITDFSVTKNKIIQLCLDLTTTVQKDCFVAEMEDKVLTVVKVLNGICDKTIRSTTDPVMSQCACLEEVHLPNIKPGEGLGMYIKSTYDGLHVITGTTENSPADRSQKIHAGDEVIQVNQQTVVGWQLKNLVKKLRENPTGVVLLLKKRPTGSFNFTPAPLKNLRWKPPLVQTSPPPATTQSPESTMDTSLKKEKSAILDLYIPPPPAV
-
Predicted Molecular Mass
66.7 kDa
-
Molecular Weight
Approximately 53-65 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
-
Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz
