Copy Link Print Information

Contactin-1/CNTN1 Protein, Mouse (HEK293, His)

Customer Review

Based on 1 Customer Validation

Contactin-1/CNTN1 protein mediates surface interactions during nervous system development. It forms paranodal axo-glial junctions in peripheral nerves and facilitates axon-glia signaling through CNTNAP1. It acts as a ligand for NOTCH1, promoting NOTCH1 activation. Contactin-1/CNTN1 also interacts with TNR, inhibiting neurite outgrowth. It binds to CNTNAP1 and PTPRZ1, and forms a complex with NRCAM, PTPRB, and TASOR. Contactin-1/CNTN1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Contactin-1/CNTN1 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Contactin-1/CNTN1 Protein, Mouse (HEK293, His) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download

To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

Contactin-1/CNTN1 protein mediates surface interactions during nervous system development. It forms paranodal axo-glial junctions in peripheral nerves and facilitates axon-glia signaling through CNTNAP1. It acts as a ligand for NOTCH1, promoting NOTCH1 activation. Contactin-1/CNTN1 also interacts with TNR, inhibiting neurite outgrowth. It binds to CNTNAP1 and PTPRZ1, and forms a complex with NRCAM, PTPRB, and TASOR. Contactin-1/CNTN1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Contactin-1/CNTN1 protein, expressed by HEK293 , with C-His labeled tag.

Background

Contactin-1/CNTN1 protein plays a crucial role in mediating cell surface interactions during nervous system development. It is involved in the formation of paranodal axo-glial junctions in myelinated peripheral nerves and facilitates signaling between axons and myelinating glial cells through its association with CNTNAP1. This protein also participates in the generation of oligodendrocytes by acting as a ligand for NOTCH1. The interaction between Contactin-1/CNTN1 and NOTCH1 promotes the activation of NOTCH1 through the release of the notch intracellular domain (NICD) and subsequent translocation to the nucleus. Additionally, Contactin-1/CNTN1 interacts with TNR, leading to neuronal repulsion and inhibition of neurite outgrowth. It exists as a monomer and interacts with CNTNAP1 in a cis form, while binding to the carbonic-anhydrase like domain of PTPRZ1. Furthermore, Contactin-1/CNTN1 is detected in a complex with NRCAM and PTPRB and interacts with TASOR.

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag C-His
  • Accession

    P12960 (D21-L1000)

  • Gene ID
    12805  [NCBI]
  • Molecular Construction
    • N-term
    • CNTN1 (D21-L1000)
      Accession # P12960
    • His
    • C-term
  • Protein Length

    Full Length of Contactin-1 Chain

  • Synonyms

    CNTN1; Contactin-1; Contactin 1; Glycoprotein Gp135; GP135; CMYO12; F3; CMYP12; Neural Cell Surface Protein F3; MYPCN; Glycoprotein GP135

  • AA Sequence

    DFTWHRRYGHGVSEEDKGFGPIFEEQPINTIYPEESLEGKVSLNCRARASPFPVYKWRMNNGDVDLTNDRYSMVGGNLVINNPDKQKDAGVYYCLASNNYGMVRSTEATLSFGYLDPFPPEERPEVKVKEGKGMVLLCDPPYHFPDDLSYRWLLNEFPVFITMDKRRFVSQTNGNLYIANVESSDRGNYSCFVSSPSITKSVFSKFIPLIPIPERTTKPYPADIVVQFKDIYTMMGQNVTLECFALGNPVPDIRWRKVLEPMPSTAEISTSGAVLKIFNIQLEDEGLYECEAENIRGKDKHQARIYVQAFPEWVEHINDTEVDIGSDLYWPCIATGKPIPTIRWLKNGYSYHKGELRLYDVTFENAGMYQCIAENAYGSIYANAELKILALAPTFEMNPMKKKILAAKGGRVIIECKPKAAPKPKFSWSKGTEWLVNSSRILIWEDGSLEINNITRNDGGIYTCFAENNRGKANSTGTLVITNPTRIILAPINADITVGENATMQCAASFDPALDLTFVWSFNGYVIDFNKEITHIHYQRNFMLDANGELLIRNAQLKHAGRYTCTAQTIVDNSSASADLVVRGPPGPPGGLRIEDIRATSVALTWSRGSDNHSPISKYTIQTKTILSDDWKDAKTDPPIIEGNMESAKAVDLIPWMEYEFRVVATNTLGTGEPSIPSNRIKTDGAAPNVAPSDVGGGGGTNRELTITWAPLSREYHYGNNFGYIVAFKPFDGEEWKKVTVTNPDTGRYVHKDETMTPSTAFQVKVKAFNNKGDGPYSLVAVINSAQDAPSEAPTEVGVKVLSSSEISVHWKHVLEKIVESYQIRYWAGHDKEAAAHRVQVTSQEYSARLENLLPDTQYFIEVGACNSAGCGPSSDVIETFTRKAPPSQPPRIISSVRSGSRYIITWDHVVALSNESTVTGYKILYRPDGQHDGKLFSTHKHSIEVPIPRDGEYVVEVRAHSDGGDGVVSQVKISGVSTL

  • Predicted Molecular Mass

    110.5 kDa

  • Molecular Weight

    Approximately 110-120 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) Volume (to add to vial)
=
Mass (in vial) Mass (in vial)
÷
Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

Concentration (start) Concentration (start)
×
Volume (start) Volume (start)
=
Concentration (final) Concentration (final)
×
Volume (final) Volume (final)
The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • Contactin-1/CNTN1 Protein, Mouse (HEK293, His) Product Image
    1/16
  • Contactin-1/CNTN1 Protein, Mouse (HEK293, His) Product Image