Contactin-3/CNTN3 Protein, Human (N708D, HEK293, His)

Contactin-3/CNTN3 protein, a member of the contactin family, facilitates cell surface interactions crucial for nervous system development. It exhibits neurite outgrowth-promoting activity, contributing to the intricate processes involved in the formation and maturation of neural circuits. Additionally, Contactin-3/CNTN3 interacts with PTPRG, indicating its involvement in signaling pathways that govern neural cell communication and connectivity. Contactin-3/CNTN3 Protein, Human (N708D, HEK293, His) is the recombinant human-derived Contactin-3/CNTN3 protein, expressed by HEK293 , with C-His labeled tag. The total length of Contactin-3/CNTN3 Protein, Human (N708D, HEK293, His) is 1002 a.a., with molecular weight of 125-135 kDa.

Contactin-3 (CNTN3) is a protein that plays a crucial role in mediating cell surface interactions during nervous system development. Functionally, it exhibits neurite outgrowth-promoting activity, suggesting its involvement in promoting the extension of neuronal processes (By similarity). Additionally, CNTN3 interacts with PTPRG, indicating a potential role in modulating signaling pathways or cellular adhesion during neural development. The complex interplay between Contactin-3 and its interacting partners underscores its significance in the intricate processes of nervous system development, where cell surface interactions and neurite outgrowth are essential for the establishment of functional neural circuits.

Measured by its binding ability in a functional ELISA. When Recombinant Huamn CNTN3 is present at 2 μg/mL, can bind Recombinant Human Amyloid Precursor. The ED₅₀ for this effect is 0.093 μg/mL.

Human

HEK293

C-10*His

Q9P232 (E20-S1002, N708D)

5067  [NCBI]

ELLLQGPVFIKEPSNSIFPVGSEDKKITLHCEARGNPSPHYRWQLNGSDIDMSMEHRYKLNGGNLVVINPNRNWDTGTYQCFATNSLGTIVSREAKLQFAYLENFKTKMRSTVSVREGQGVVLLCGPPPHSGELSYAWIFNEYPSFVEEDSRRFVSQETGHLYISKVEPSDVGNYTCVVTSMVTNARVLGSPTPLVLRSDGVMGEYEPKIEVQFPETLPAAKGSTVKLECFALGNPIPQINWRRSDGLPFSSKIKLRKFSGVLEIPNFQQEDAGSYECIAENSRGKNVARGRLTYYAKPHWVQLIKDVEIAVEDSLYWECRASGKPKPSYRWLKNGAALVLEERTQIENGALTISNLSVTDSGMFQCIAENKHGLVYSSAELKVVASAPDFSKNPMKKLVQVQVGSLVSLDCKPRASPRALSSWKKGDVSVQEHERISLLNDGGLKIANVTKADAGTYTCMAENQFGKANGTTHLVVTEPTRITLAPSNMDVSVGESVILPCQVQHDPLLDIIFTWYFNGALADFKKDGSHFEKVGGSSSGDLMIRNIQLKHSGKYVCMVQTGVDSVSSAADLIVRGSPGPPENVKVDEITDTTAQLSWKEGKDNHSPVISYSIQARTPFSVGWQTVTTVPEVIDGKTHTATVVELNPWVEYEFRVVASNKIGGGEPSLPSEKVRTEEAVPEVPPSEVDGGGGSRSELVITWDPVPEELQNGEGFGYVVAFRPLGVTTWIQTVVTSPDTPRYVFRNESIVPYSPYEVKVGVYNNKGEGPFSPVTTVFSAEEEPTVAPSQVSANSLSSSEIEVSWNTIPWKLSNGHLLGYEVRYWNGGGKEESSSKMKVAGNETSARLRGLKSNLAYYTAVRAYNSAGAGPFSATVNVTTKKTPPSQPPGNVVWNATDTKVLLNWEQVKAMENESEVTGYKVFYRTSSQNNVQVLNTNKTSAELVLPIKEDYIIEVKATTDGGDGTSSEQIRIPRITSMDARGS

Approximately 125-135 kDa due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.