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Contactin-3/CNTN3 Protein, Human (N708D, HEK293, His)

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Based on 1 Customer Validation

Contactin-3/CNTN3 protein, a member of the contactin family, facilitates cell surface interactions crucial for nervous system development. It exhibits neurite outgrowth-promoting activity, contributing to the intricate processes involved in the formation and maturation of neural circuits. Additionally, Contactin-3/CNTN3 interacts with PTPRG, indicating its involvement in signaling pathways that govern neural cell communication and connectivity. Contactin-3/CNTN3 Protein, Human (N708D, HEK293, His) is the recombinant human-derived Contactin-3/CNTN3 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Contactin-3/CNTN3 Protein, Human (N708D, HEK293, His)
  • Experimental Validation Results for Contactin-3/CNTN3 Protein, Human (N708D, HEK293, His)
  • Contactin-3/CNTN3 Protein, Human (N708D, HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

Contactin-3/CNTN3 protein, a member of the contactin family, facilitates cell surface interactions crucial for nervous system development. It exhibits neurite outgrowth-promoting activity, contributing to the intricate processes involved in the formation and maturation of neural circuits. Additionally, Contactin-3/CNTN3 interacts with PTPRG, indicating its involvement in signaling pathways that govern neural cell communication and connectivity. Contactin-3/CNTN3 Protein, Human (N708D, HEK293, His) is the recombinant human-derived Contactin-3/CNTN3 protein, expressed by HEK293 , with C-His labeled tag.

Background

Contactin-3 (CNTN3) is a protein that plays a crucial role in mediating cell surface interactions during nervous system development. Functionally, it exhibits neurite outgrowth-promoting activity, suggesting its involvement in promoting the extension of neuronal processes (By similarity). Additionally, CNTN3 interacts with PTPRG, indicating a potential role in modulating signaling pathways or cellular adhesion during neural development. The complex interplay between Contactin-3 and its interacting partners underscores its significance in the intricate processes of nervous system development, where cell surface interactions and neurite outgrowth are essential for the establishment of functional neural circuits.

Verified Bioactivity

Measured by its binding ability in a functional ELISA. When Recombinant Huamn CNTN3 is present at 2 μg/mL, can bind Recombinant Human Amyloid Precursor. The ED50 for this effect is 0.093 μg/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Measured by its binding ability in a functional ELISA. When Recombinant Huamn CNTN3 is present at 2 μg/mL, can bind Recombinant Human Amyloid Precursor. The ED50 for this effect is 0.093 μg/mL.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-10*His
  • Accession

    Q9P232/NP_065923.1 (E20-S1002, N708D)

  • Gene ID
    5067  [NCBI]
  • Molecular Construction
    • N-term
    • CNTN3 (E20-S1002, N708D)
      Accession # Q9P232/NP_065923.1
    • His
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    CNTN3; Plasmacytoma-Associated Neuronal Glycoprotein; Prev. PANG; Contactin-3; BIG-1; KIAA1496; Brain-Derived Immunoglobulin Superfamily Protein 1; PCS; Contactin 3; Contactin 3 (Plasmacytoma Associated)

  • AA Sequence

    ELLLQGPVFIKEPSNSIFPVGSEDKKITLHCEARGNPSPHYRWQLNGSDIDMSMEHRYKLNGGNLVVINPNRNWDTGTYQCFATNSLGTIVSREAKLQFAYLENFKTKMRSTVSVREGQGVVLLCGPPPHSGELSYAWIFNEYPSFVEEDSRRFVSQETGHLYISKVEPSDVGNYTCVVTSMVTNARVLGSPTPLVLRSDGVMGEYEPKIEVQFPETLPAAKGSTVKLECFALGNPIPQINWRRSDGLPFSSKIKLRKFSGVLEIPNFQQEDAGSYECIAENSRGKNVARGRLTYYAKPHWVQLIKDVEIAVEDSLYWECRASGKPKPSYRWLKNGAALVLEERTQIENGALTISNLSVTDSGMFQCIAENKHGLVYSSAELKVVASAPDFSKNPMKKLVQVQVGSLVSLDCKPRASPRALSSWKKGDVSVQEHERISLLNDGGLKIANVTKADAGTYTCMAENQFGKANGTTHLVVTEPTRITLAPSNMDVSVGESVILPCQVQHDPLLDIIFTWYFNGALADFKKDGSHFEKVGGSSSGDLMIRNIQLKHSGKYVCMVQTGVDSVSSAADLIVRGSPGPPENVKVDEITDTTAQLSWKEGKDNHSPVISYSIQARTPFSVGWQTVTTVPEVIDGKTHTATVVELNPWVEYEFRVVASNKIGGGEPSLPSEKVRTEEAVPEVPPSEVDGGGGSRSELVITWDPVPEELQNGEGFGYVVAFRPLGVTTWIQTVVTSPDTPRYVFRNESIVPYSPYEVKVGVYNNKGEGPFSPVTTVFSAEEEPTVAPSQVSANSLSSSEIEVSWNTIPWKLSNGHLLGYEVRYWNGGGKEESSSKMKVAGNETSARLRGLKSNLAYYTAVRAYNSAGAGPFSATVNVTTKKTPPSQPPGNVVWNATDTKVLLNWEQVKAMENESEVTGYKVFYRTSSQNNVQVLNTNKTSAELVLPIKEDYIIEVKATTDGGDGTSSEQIRIPRITSMDARGS

  • Molecular Weight

    Approximately 120-130 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. When Recombinant Huamn CNTN3 is present at 2 μg/mL, can bind Recombinant Human Amyloid Precursor. The ED50 for this effect is 0.093 μg/mL.

  • Contactin-3/CNTN3 Protein, Human (N708D, HEK293, His) Product Image
    1/16
  • Experimental Validation Results for Contactin-3/CNTN3 Protein, Human (N708D, HEK293, His)
  • Experimental Validation Results for Contactin-3/CNTN3 Protein, Human (N708D, HEK293, His)
  • Contactin-3/CNTN3 Protein, Human (N708D, HEK293, His) Product Image