DAP10 Protein, Human (HEK293, mFc)

DAP10 is a transmembrane adapter protein that forms the activating receptor KLRK1-HCST, which is critical for cytotoxicity in target cells expressing stress-inducing ligands. It is activated by ULBP, triggering calcium mobilization and activating PI3K, MAP2K/ERK and JAK2/STAT5 signaling pathways. DAP10 Protein, Human (HEK293, mFc) is the recombinant human-derived DAP10 protein, expressed by HEK293 , with C-mFc labeled tag.

DAP10, a transmembrane adapter protein, forms an activation receptor, KLRK1-HCST, in lymphoid and myeloid cells, playing a pivotal role in triggering cytotoxicity against target cells expressing stress-induced ligands such as MHC class I chain-related MICA and MICB, as well as UL16-binding proteins (ULBPs). This receptor, activated by the interaction of ULBPs with KLRK1-HCST, initiates calcium mobilization and activates the PI3-kinase PIK3R1, MAP2K/ERK, and JAK2/STAT5 signaling pathways. The presence of both PIK3R1 and GRB2 is essential for full KLRK1-HCST-mediated activation, leading to the ultimate killing of target cells. In NK cells, KLRK1-HCST signaling induces cytotoxicity and enhances cytokine production, while in T-cells, it primarily provides costimulation for TCR-induced signals. The KLRK1-HCST receptor plays a crucial role in immune surveillance against tumors, and its absence results in the escape of melanoma cells from NK cell-mediated immune surveillance. DAP10 interacts with CLEC5A, forming a CLEC5A/TYROBP/HCST trimolecular complex, and it homodimerizes, with disulfide linkages. The heterohexamer comprises four subunits of HCST/DAP10 and two subunits of KLRK1, and it interacts with PIK3R1, GRB2, KLRK1, and CD300H.

Human

HEK293

C-mFc

Q9UBK5 (Q19-P48)

10870  [NCBI]

Extracellular Domain

QTTPGERSSLPAFYPGTSGSCSGCGSLSLP

Approximately 33-45 kDa

• 
  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.