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EPHA5 Protein, Cynomolgus (HEK293, His)

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EPHA5 is a member of the erythropoietin-producing human liver cancer receptor (Eph receptor) that regulates embryogenesis by fine-tuning cell adhesion, localization, and migration, particularly in the nervous system. EPHA5 enhances the invasion and migration of esophageal squamous cell carcinoma through epithelial-mesenchymal transformation by activating the Wnt/β-catenin pathway. EPHA5 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived EPHA5 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Cynomolgus
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • EPHA5 Protein, Cynomolgus (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

EPHA5 is a member of the erythropoietin-producing human liver cancer receptor (Eph receptor) that regulates embryogenesis by fine-tuning cell adhesion, localization, and migration, particularly in the nervous system. EPHA5 enhances the invasion and migration of esophageal squamous cell carcinoma through epithelial-mesenchymal transformation by activating the Wnt/β-catenin pathway. EPHA5 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived EPHA5 protein, expressed by HEK293 , with C-His labeled tag.

Background

Erythropoietin-producing human liver cancer receptors (Eph receptors) are the largest subgroup of receptor tyrosine kinases. Eph receptors were originally found to regulate embryogenesis by fine-tuning cell adhesion, localization, and migration, particularly in the nervous system. EPHA5 is a member of the Eph receptor and is involved in a variety of biological activities, including tumorigenesis and the progression of different cancers. EPHA5 enhances the invasion and migration of esophageal squamous cell carcinoma through epithelial-mesenchymal transformation by activating the Wnt/β-catenin pathway[1][2][3].

Verified Bioactivity

This product does not contain protein kinase domain.

Technical Parameters

  • Species Cynomolgus
  • Source HEK293
  • Tag C-8*His
  • Accession

    A0A2K5W6J6 (P25-Q572)

  • Gene ID
    102138762  [NCBI]
  • Molecular Construction
    • N-term
    • EPHA5 (P25-Q572)
      Accession # A0A2K5W6J6
    • 8*His
    • C-term
  • Protein Length

    Partial

  • Synonyms

    EPHA5; EHK-1; EPH Receptor A5; EK7; TYRO4; Epididymis Secretory Sperm Binding Protein; EHK1; Tyrosine-Protein Kinase Receptor EHK-1; Hek7; Receptor Protein-Tyrosine Kinase HEK7; CEK7; Receptor Protein-Tyrosine Kinase; Ephrin Type-A Receptor 5; EphA5; EPH

  • AA Sequence

    PASLAGCYSAPRRAPLWTCLLLCAALRTLLASPSNEVNLLDSRTVMGDLGWIAFPKNGWEEIGEVDENYAPIHTYQVCKVMEQNQNNWLLTSWISNEGASRIFIELKFTLRDCNSLPGGLGTCKETFNMYYFESDDQNGRNIKENQYIKIDTIAADESFTELDLGDRVMKLNTEVRDVGPLSKKGFYLAFQDVGACIALVSVRVYYKKCPSVVRHLAVFPDTITGADSSQLLEVSGSCVNHSVTDEPPKMHCSAEGEWLVPIGKCMCKAGYEEKNGTCQVCRPGFFKASPHIQSCGKCPPHSYTHEEASTSCVCEKDYFRRESDPPTMACTRPPSAPRNAISNINETSVFLEWIPPADTGGRKDVSYYIACKKCNSHAGVCEECGGHVRYLPRQSGLKNTSVMMVDLLAHTNYTFEIEAVNGVSDLSPGARQYVSVNVTTNQAAPSPVTNVKKGKIAKNSISLSWQEPDRPNGIILEYEIKYFEKDQETSYTIIKSKETTITAEGLKPASVYVFQIRARTAAGYGVFSRRFEFETTPVSVAASSDQSQ

  • Predicted Molecular Mass

    61.7 kDa

  • Molecular Weight

    Approximately 70-75kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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