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  6. FGFR-1 alpha (IIIc) Protein, Human (Biotinylated, HEK293, His-Avi)

FGFR-1 alpha (IIIc) Protein, Human (Biotinylated, HEK293, His-Avi)

Cat. No.: HY-P77660
COA Handling Instructions

FGFR-1 alpha is a tyrosine-protein kinase receptor for fibroblast growth factor that is critical in embryonic development, cell proliferation, differentiation, and migration. GnRH is essential for mesodermal patterning, axial organization, skeletogenesis, and nervous system development. FGFR-1 alpha (IIIc) Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived FGFR-1 alpha, expressed by HEK293 , with C-Avi, C-His labeled tag. The total length of FGFR-1 alpha (IIIc) Protein, Human (Biotinylated, HEK293, His-Avi) is 353 a.a., with molecular weight of 68-80 kDa.

For research use only. We do not sell to patients.

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Description

FGFR-1 alpha is a tyrosine-protein kinase receptor for fibroblast growth factor that is critical in embryonic development, cell proliferation, differentiation, and migration. GnRH is essential for mesodermal patterning, axial organization, skeletogenesis, and nervous system development. FGFR-1 alpha (IIIc) Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived FGFR-1 alpha, expressed by HEK293 , with C-Avi, C-His labeled tag. The total length of FGFR-1 alpha (IIIc) Protein, Human (Biotinylated, HEK293, His-Avi) is 353 a.a., with molecular weight of 68-80 kDa.

Background

FGFR-1 alpha, a tyrosine-protein kinase, functions as a cell-surface receptor for fibroblast growth factors and plays a pivotal role in regulating embryonic development, cell proliferation, differentiation, and migration. It is essential for normal mesoderm patterning, proper axial organization during embryonic development, skeletogenesis, and the development of the gonadotropin-releasing hormone (GnRH) neuronal system. Upon ligand binding, FGFR-1 alpha activates multiple signaling cascades, phosphorylating key proteins such as PLCG1, FRS2, GAB1, and SHB. This activation leads to the production of signaling molecules like diacylglycerol and inositol 1,4,5-trisphosphate through PLCG1. Moreover, phosphorylation of FRS2 triggers the recruitment of GRB2, GAB1, PIK3R1, and SOS1, mediating the activation of RAS, MAPK1/ERK2, MAPK3/ERK1, the MAP kinase signaling pathway, and the AKT1 signaling pathway. FGFR-1 alpha also promotes the phosphorylation of SHC1, STAT1, and PTPN11/SHP2. Within the nucleus, it enhances the activity of RPS6KA1 and CREB1, contributing to the regulation of transcription. The down-regulation of FGFR-1 alpha signaling occurs through IL17RD/SEF and FGFR-1 alpha ubiquitination, internalization, and degradation.

Biological Activity

Immobilized Biotinylated Human FGFR1 alpha (IIIc) at 1 μg/mL (100 μl/well) on the plate. Dose response curve for Anti-FGFRI Anitibody, hFc Tag with the EC50 <11 ng/mL determined by ELISA.

Species

Human

Source

HEK293

Tag

C-Avi;C-His

Accession

P11362-7 (R22-E374)

Gene ID
Molecular Construction
N-term
FGFR-1 alpha (IIIc) (R22-E374)
Accession # P11362-7
His-Avi
C-term
Synonyms
FGF R1a; FGFR1 alpha
Molecular Weight

65-90 kDa

Purity

Greater than 95% as determined by Tris-Bis PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
FGFR-1 alpha (IIIc) Protein, Human (Biotinylated, HEK293, His-Avi)
Cat. No.:
HY-P77660
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