FGL1 Protein, Cynomolgus (HEK293, His)

Fibrinogen like 1 (FGL1) is a proliferation- and metabolism-related protein secreted by the liver. FGL1 is also upregulated in tumor tissues. FGL1 is a checkpoint ligand of lymphocyte activation gene 3 (LAG3). When binding to LAG3, FGL1 can inhibit T cell activation and proliferation. FGL1 can be used for research of immune checkpoint therapy. FGL1 plays a role in proliferation, metabolism, apoptosis, epithelial-to-mesenchymal transition, and immune infiltration^[1][2][3][4]. FGL1 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived FGL1 protein, expressed by HEK293 , with C-6*His labeled tag.

Fibrinogen like 1 (FGL1), also known as HFREP-1 or hepassocin (HPS), is a proliferation- and metabolism-related protein secreted by the liver. FGL1 is a newly emerging checkpoint ligand of lymphocyte activation gene 3 (LAG3). When binding to LAG3, it can inhibit T cell activation and proliferation. Therefore, FGL1 can potentiate anti-tumor T cell responses and can be used for research of immune checkpoint therapy. Apart from the high expression in the liver, FGL1 is also upregulated in tumor tissues (such as lung, prostate, melanoma, colorectal, breast and brain tumors) and mediates EMT process. FGL1 is an approxiamte 68-KD protein comprised of a disulfide bond-linked homodimer^[1][2][3].
Besides, as a liver protective factor, FGL1 accelerates the growth of liver cells by promoting mitochondrial mitosis in the event of liver injury. FGL1 plays a role in proliferation, metabolism, apoptosis, epithelial-to-mesenchymal transition, and immune infiltration^[3][4].

Cynomolgus

HEK293

C-6*His

A0A2K5X990 (L23-I312)

102118020

Full Length of Mature Protein

LEDCAQEQVRLRAQVRLLETRVKQQQVKIKQLLQENEVQFLDKGEENSVIDLGSKRQYADCSEIFNDGYKLSGFYKIKPLQSPAEFAVYCDMSDGGGWTVIQRRSDGSENFNRGWNDYENGFGNFVQKHGEYWLGNKNLHFLTTQEDYTLKIDLADFEKNSRYAQYKNFKVGDEKNFYELNIGEYSGTAGDSLAGSFHPEVQWWATHQRMKFSTWDRDHDNYDGNCAEEDQSGWWFNRCHSANLNGLYYTGPYTAKTDNGIVWYTWHGWWYSLKSVVMKIRPNDFIPNVI

Approximately 35 kDa,based on SDS-PAGE under reducing conditions.

≥ 95%, as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of 20 mM Tris, 300 mM NaCl, 10% Glycerol, 2 mM EDTA, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.