GPRC5D Protein-Nanodisc, Cynomolgus (HEK293, His, Flag)

GPRC5D is a G protein-coupled orphan receptor that is homologous to the fifth member of the C family of mammalian G protein-coupled receptors (GPCRs) (related to metabotropic glutamate receptors). GPRC5D is a target for CAR-T cell immunotherapy research in multiple myeloma. GPRC5D Protein-Nanodisc, Cynomolgus (HEK293, His, Flag) is a recombinant GPRC5D protein expressed in HEK293 cells, with C-terminal His and N-terminal Flag tags.

G protein-coupled receptor class C 5 member D (GPRC5D) is a G protein-coupled orphan receptor that shares homology with mammalian G protein-coupled receptors (GPCRs) superfamily C family group 5 member (related to metabotropic glutamate receptor-like) and belongs to the RAIG1 family along with two highly homologous orphan G protein-coupled receptors (oGPCRs), GPRC5B and GPRC5C (RAIG1 was originally isolated as an orphan GPCR by differential display, and its expression can be induced by all-trans retinoic acid (ATRA) in human head and neck squamous cell carcinoma (HNSCC)). As a member of the G protein-coupled receptor that only exists in eukaryotes (including yeast and flagella), GPRC5D is mainly expressed on malignant plasma cells, but limitedly expressed in normal tissues. It is a potential therapeutic target for relapsed/refractory multiple myeloma (R/R MM). Its targeted CAR-T, CAR-NK cell therapy and bispecific T cell engagers have significant anti-tumor activity, and it is also a target for CAR-T cell immunotherapy of multiple myeloma. In addition, G protein-coupled receptors can be bound and activated by a variety of ligands (ranging in size from small molecules to large proteins), such as photosensitive compounds, odors, and hormones, and are associated with a variety of diseases. Its signal transduction pathways include the cAMP signaling pathway and the phosphatidylinositol signaling pathway.

Cynomolgus

HEK293

C-His;N-Flag

A0A2K5W6I7 (M1-F192)&(E191-L262)

102122120

Approximately 37 kDa, based on SDS-PAGE under reducing conditions.

≥ 80%, as determined by reducing SDS-PAGE.

Solution.

Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, pH7.5 with glycerol as protectant.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. Smith EL, et al. GPRC5D is a target for the immunotherapy of multiple myeloma with rationally designed CAR T cells. Sci Transl Med. 2019 Mar 27;11(485):eaau7746.  [Content Brief]

  [2]. King N, et al. Evolution of key cell signaling and adhesion protein families predates animal origins. Science. 2003 Jul 18;301(5631):361-3.  [Content Brief]

  [3]. Gilman AG. G proteins: transducers of receptor-generated signals. Annu Rev Biochem. 1987;56:615-49.  [Content Brief]