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  2. Viral Proteins
  3. HCoV-229E Spike/S Protein (APT69883, sf9, His)

HCoV-229E Spike/S Protein (APT69883, sf9, His)

Cat. No.: HY-P76384
Handling Instructions

Coronaviruses (CoVs) are enveloped, positive-sense, single-stranded RNA viruses with the largest genomes known among RNA viruses. The CoVs S-protein mediates receptor binding and fusion of the viral and host cell membranes. HCoV-229E is an alpha-coronaviruse that uses different host proteins as receptors.In HCoV-229E, its receptor binding domain (RBD) shows extensive sequence variation. HCoV-229E exploits trypsin, cathepsin L and TMPRSS2 to complete the fusion activation mediated by the S protein. HCoV-229E Spike/S Protein (APT69883, sf9, His) is the recombinant Virus-derived HCoV-229E Spike/S protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of HCoV-229E Spike/S Protein (APT69883, sf9, His) is 1100 a.a., with molecular weight of ~122.1 KDa.

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Description

Coronaviruses (CoVs) are enveloped, positive-sense, single-stranded RNA viruses with the largest genomes known among RNA viruses. The CoVs S-protein mediates receptor binding and fusion of the viral and host cell membranes. HCoV-229E is an alpha-coronaviruse that uses different host proteins as receptors.In HCoV-229E, its receptor binding domain (RBD) shows extensive sequence variation. HCoV-229E exploits trypsin, cathepsin L and TMPRSS2 to complete the fusion activation mediated by the S protein[1][2]. HCoV-229E Spike/S Protein (APT69883, sf9, His) is the recombinant Virus-derived HCoV-229E Spike/S protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of HCoV-229E Spike/S Protein (APT69883, sf9, His) is 1100 a.a., with molecular weight of ~122.1 KDa.

Background

Coronaviruses (CoVs) are enveloped, positive-sense, single-stranded RNA viruses with the largest genomes known among RNA viruses (~26–32 kb) belonging to the order Nidovirales1. Coronaviruses spike (S) glycoproteins mediate viral entry into host cells by binding to host receptors. To date, there are seven coronaviruses, namely the alphacoronaviruses HCoV-229E and HCoV-NL63, the betacoronaviruses HCoV-OC43, HCoV-HKU1, SARS-CoV, MERS-CoV and the emerging coronavirus (SARS-CoV-2). Moreover, fusion activation of HCoV-229E may be highly reliant on cleavage of the S2´ trigger loop (GSR685↓V686AG)[1][2].

Species

Virus

Source

Sf9 insect cells

Tag

C-His

Accession

APT69883 (C16-W1115)

Gene ID

/

Molecular Construction
N-term
HCoV-229E S (C16-W1115)
Accession # APT69883
His
C-term
Synonyms
Human coronAvirus (HCoV-229E) Spike Protein (S1+S2 ECD, His)
Molecular Weight

Approximately 122.1 kDa.

Purity

Greater than 85% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

HCoV-229E Spike/S Protein (APT69883, sf9, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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HCoV-229E Spike/S Protein (APT69883, sf9, His)
Cat. No.:
HY-P76384
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