HCoV-229E Spike/S Protein (APT69883, sf9, His)
Coronaviruses (CoVs) are enveloped, positive-sense, single-stranded RNA viruses with the largest genomes known among RNA viruses. The CoVs S-protein mediates receptor binding and fusion of the viral and host cell membranes. HCoV-229E is an alpha-coronaviruse that uses different host proteins as receptors.In HCoV-229E, its receptor binding domain (RBD) shows extensive sequence variation. HCoV-229E exploits trypsin, cathepsin L and TMPRSS2 to complete the fusion activation mediated by the S protein. HCoV-229E Spike/S Protein (APT69883, sf9, His) is the recombinant Virus-derived HCoV-229E Spike/S protein, expressed by Sf9 insect cells , with C-His labeled tag.
- Species: Virus
- Source: Sf9 insect cells
-
Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Technical Parameters
-
Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
Coronaviruses (CoVs) are enveloped, positive-sense, single-stranded RNA viruses with the largest genomes known among RNA viruses. The CoVs S-protein mediates receptor binding and fusion of the viral and host cell membranes. HCoV-229E is an alpha-coronaviruse that uses different host proteins as receptors.In HCoV-229E, its receptor binding domain (RBD) shows extensive sequence variation. HCoV-229E exploits trypsin, cathepsin L and TMPRSS2 to complete the fusion activation mediated by the S protein[1][2]. HCoV-229E Spike/S Protein (APT69883, sf9, His) is the recombinant Virus-derived HCoV-229E Spike/S protein, expressed by Sf9 insect cells , with C-His labeled tag.
Background
Coronaviruses (CoVs) are enveloped, positive-sense, single-stranded RNA viruses with the largest genomes known among RNA viruses (~26–32 kb) belonging to the order Nidovirales1. Coronaviruses spike (S) glycoproteins mediate viral entry into host cells by binding to host receptors. To date, there are seven coronaviruses, namely the alphacoronaviruses HCoV-229E and HCoV-NL63, the betacoronaviruses HCoV-OC43, HCoV-HKU1, SARS-CoV, MERS-CoV and the emerging coronavirus (SARS-CoV-2). Moreover, fusion activation of HCoV-229E may be highly reliant on cleavage of the S2´ trigger loop (GSR685↓V686AG)[1][2].
Technical Parameters
-
Species Virus
-
Source Sf9 insect cells
-
Tag C-His
-
Accession
APT69883 (C16-W1115)
-
Gene ID/
-
Molecular Construction
-
N-term
-
HCoV-229E S (C16-W1115)
Accession # APT69883 -
His
-
C-term
-
-
Protein Length
Partial
-
Synonyms
Human coronAvirus (HCoV-229E) Spike Protein (S1+S2 ECD, His)
-
AA Sequence
CQTTNGTNTSHSVCNGCVGHSENVFAVESGGYIPSNFSFNNWFLLTNTSSVVDGVVRSFQPLLLNCLWSVSGSQFTTGFVYFNGTGRGACKGFYSNASSDVIRYNINFEENLRRGTILFKTSYGAVVFYCTNNTLVSGDAHIPSGTVLGNFYCFVNTTIGNETTSAFVGALPKTVREFVISRTGHFYINGYRYFSLGDVEAVNFNVTNAATIVCTVALASYADVLVNVSQTAIANIIYCNSVINRLRCDQLSFDVPDGFYSTSPIQPVELPMSIVSLPVYHKHTFIVLHVKFEHQRGPGKCYNCRPSVINITLANFNETKGPLCVDTSHFTTQFVDNVKLARWSASINTGNCPFSFGKVNNFVKFGSVCFSLKDIPGGCAMPIMANLVNHKSHNIGSLYVSWSDGDVITGVPKPVEGVSSFMNVTLNKCTKYNIYDVSGVGVIRISNDTFLNGITYTSTSGNLLGFKDVTNGTIYSITPCNPPDQLVVYQQAVVGAMLSENFTSYGFSNVVEMPKFFYASNGTYNCTDAVLTYSSFGVCADGSIIAVQPRNVSYDSVSAIVTANLSIPSNWTTSVQVEYLQITSKPIVVDCSTYVCNGNVRCVELLKQYTSACKTIEDALRNSAMLESADVSEMLTFDKKAFTLANVSSFGDYNLSSVIPSLPRSGSRVAGRSAIEDILFSKLVTSGLGTVDADYKKCTKGLSIADLACAQYYNGIMVLPGVADAERMAMYTGSLIGGIALGGLTSAASIPFSLAIQSRLNYVALQTDVLQENQKILAASFNKAMTNIVDAFTGVNDAITQTSQALQTVATALNKIQDVVNQQGNSLNHLTSQLRQNFQAISSSIQAIYDRLDIIQADQQVDRLITGRLAALNVFVSHTLTKYTEVRASRQLAQQKVNECVKSQSKRYGFCGNGTHIFSLVNAAPEGLVFLHTVLLPTQYKDVEAWSGLCVDGINGYVLRQPNLALYKEGNYYRITSRIMFEPRIPTIADFVQIENCNVTFVNISRSELQTIVPEYIDVNKTLQELSYKLPNYTVPDLVVEQYNQTILNLTSEISTLENKSAELNYTVQKLQTLIDNINSTLVDLKWLNRVETYIKWPWW
-
Predicted Molecular Mass
122.1 kDa
-
Purity
≥ 85%, as determined by reducing SDS-PAGE.
Product Properties
Solution
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz
